Identification of two major QTL for yellow seed color in two crosses of resynthesized Brassica napus line No. 2127-17

2010 ◽  
Vol 28 (3) ◽  
pp. 335-342 ◽  
Author(s):  
Yan Zhang ◽  
Xia Li ◽  
Wei Chen ◽  
Bin Yi ◽  
Jing Wen ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Seed Color ◽  
Yellow Seed ◽  
Resynthesized Brassica Napus ◽  
Napus Line ◽  
Major Qtl

Localization of QTLs for seed color using recombinant inbred lines of Brassica napus in different environments

Genome ◽  
2007 ◽  
Vol 50 (9) ◽  
pp. 840-854 ◽  
Author(s):  
Fu-You Fu ◽  
Lie-Zhao Liu ◽  
You-Rong Chai ◽  
Li Chen ◽  
Tao Yang ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Candidate Gene ◽  
Recombinant Inbred ◽  
Seed Color ◽  
Srap Marker ◽  
Linkage Groups ◽  
Yellow Seed ◽  
Seed Trait ◽  
Major Qtl ◽  
Selection Of

Yellow seed is one of the most important traits of Brassica napus L. Efficient selection of the yellow-seed trait is one of the most important objectives in oilseed rape breeding. Two recombinant inbred line (RIL) populations (RIL-1 and RIL-2) were analyzed for 2 years at 2 locations. Four hundred and twenty SSR, RAPD, and SRAP marker loci covering 1744 cM were mapped in 26 linkage groups of RIL-1, while 265 loci covering 1135 cM were mapped in 20 linkage groups of RIL-2. A total of 19 QTLs were detected in the 2 populations. A major QTL was detected adjacent to the same marker (EM11ME20/200) in both maps in both years. This major QTL could explain 53.71%, 39.34%, 42.42%, 30.18%, 24.86%, and 15.08% of phenotypic variation in 6 combinations (location × year × population). BLASTn analysis of the sequences of the markers flanking the major QTL revealed that the homologous region corresponding to this major QTL was anchored between genes At5g44440 and At5g49640 of Arabidopsis thaliana chromosome 5 (At C5). Based on comparative genomic analysis, the bifunctional gene TT10 is nearest to the homologue of EM11ME20/200 on At C5 and can be considered an important candidate gene for the major QTL identified here. Besides providing an effective strategy for marker-assisted selection of the yellow-seed trait in B. napus, our results also provide important clues for cloning of the candidate gene corresponding to this major QTL.

Pod shatter resistance in the resynthesized Brassica napus line DK142

2003 ◽  
Vol 140 (1) ◽  
pp. 43-52 ◽  
Author(s):  
J. E. SUMMERS ◽  
D. M. BRUCE ◽  
G. VANCANNEYT ◽  
P. REDIG ◽  
C. P. WERNER ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Dry Matter ◽  
Descriptive Statistics ◽  
Sowing Time ◽  
Commercial Cultivar ◽  
Resynthesized Brassica Napus ◽  
Pod Shatter ◽  
Napus Line ◽  
The Uk

Resistance to pod shatter was studied within and between populations of the resynthesized Brassica napus line DK142, grown under glass or in the field, at sites in the UK and Belgium. All populations showed similar ranges of resistance that were greater than that of a commercial cultivar (Apex). The increase in range was at least three-fold greater than the range found in Apex. Only sowing time affected the descriptive statistics of shatter resistance of each line, with spring-sown populations more shatter susceptible than winter-sown populations. The partitioning of dry matter to individual tissues of the pod was different for the two lines, with dry matter biased to the seed in Apex and to the receptacle in the more resistant DK142. In DK142 and Apex, as well as F1 and F2 populations derived from crosses between DK142×Apex, correlations for pod shatter resistance and mature pod characters were high, particularly the weight and length of the valves and septum. Shatter resistance increased in plants when pod numbers were reduced by the removal of whole racemes.

Colocalization of a partially dominant gene for yellow seed colour with a major QTL influencing acid detergent fibre (ADF) content in different crosses of oilseed rape (Brassica napus)

Genome ◽  
2006 ◽  
Vol 49 (12) ◽  
pp. 1499-1509 ◽  
Author(s):  
Ana Gloria Badani ◽  
Rod J. Snowdon ◽  
Benjamin Wittkop ◽  
Florin D. Lipsa ◽  
Roland Baetzel ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Dominant Gene ◽  
Fibre Content ◽  
Clear Correlation ◽  
Potential Candidate ◽  
Seed Colour ◽  
Yellow Seed ◽  
Acid Detergent Fibre ◽  
Major Qtl

Quantitative trait loci (QTLs) contributing to yellow seed colour and acid detergent fibre (ADF) were localized and compared in 3 mapping populations developed from 2 crosses (designated ‘YE1’ and ‘YE2’) between 2 distinct sources of true-breeding yellow-seeded oilseed rape (Brassica napus) and 2 different black-seeded genotypes. A clear correlation was observed between seed colour and ADF content in both crosses. In all 3 populations, a major QTL, with a large effect on both seed colour and ADF in multiple environments, was detected at the same position on chromosome N18. In YE1, a second minor QTL, with a small effect on seed colour but not on ADF content, was localized on chromosome N1. In YE2, no QTL was observed on N1; however, 2 minor seed-colour loci were localized to N15 and N5. A second major QTL for ADF was localized in YE1 on N13; in YE2, no other QTLs for ADF were detected. Combined QTL and segregation data for seed colour and ADF content in the different populations suggest that a partially dominant B. napus gene for seed colour on N18 contributes to a reduction in fibre content in different yellow-seeded B. napus genotypes. The other QTLs that were identified appear to represent different genes in the 2 yellow-seeded rapeseed sources, which, in each case, affect only fibre content or seed colour, respectively. Potential candidate genes and implications for marker-assisted breeding of oilseed rape with reduced seed dietary fibre content are discussed.

scholarly journals A High-Density Genetic Map Identifies a Novel Major QTL for Boron Efficiency in Oilseed Rape (Brassica napus L.)

PLoS ONE ◽  
2014 ◽  
Vol 9 (11) ◽  
pp. e112089 ◽  
Author(s):  
Didi Zhang ◽  
Yingpeng Hua ◽  
Xiaohua Wang ◽  
Hua Zhao ◽  
Lei Shi ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Genetic Map ◽  
Oilseed Rape ◽  
High Density ◽  
Brassica Napus L ◽  
Boron Efficiency ◽  
Major Qtl

scholarly journals Mapping and identification of yellow seed coat color genes in Brassica juncea L.

2020 ◽  
Author(s):  
Zhen Huang ◽  
Yang Wang ◽  
Hong Lu ◽  
Xiang Liu ◽  
Lu Liu ◽  
...  
Keyword(s):  
Candidate Gene ◽  
Brassica Juncea ◽  
Seed Coat ◽  
Coat Color ◽  
Seed Color ◽  
Seed Coat Color ◽  
Flavonoid Pathway ◽  
Yellow Seed ◽  
Color Formation ◽  
Yellow Seed Coat

Abstract BackgroundYellow seed breeding is an effective method to improve the oil content in rapeseed. Yellow seed coat color formation is influenced by various factors, and no clear mechanisms are known. In this study, Bulked segregant RNA-Seq (BSR-Seq) of BC9 population of Wuqi mustard (yellow seed) and Wugong mustard (brown seed) was used to identity the candidate genes controlling the yellow seed color in Brassica juncea L.ResultsYellow seed coat color gene was mapped to chromosome A09, and differentially expressed genes (DEGs) between brown and yellow bulks enriched in the flavonoid pathway. A significant correlation between the expression of BjF3H and BjTT5 and the content of the seed coat color related indexes was identified. Two intron polymorphism (IP) markers linked to the target gene were developed around BjF3H. Therefore, BjF3H was considered as the candidate gene. The BjF3H coding sequences (CDS) of Wuqi mustard and Wugong mustard are 1071-1077bp, encoding protein of 356-358 amino acids. One amino acid change (254, F/V) was identified in the conserved domain. This mutation site was detected in four Brassica rapa (B. rapa) and six Brassica juncea (B. juncea) lines, but not in Brassica napus (B. napus).ConclusionsThe results indicated BjF3H is a candidate gene that related to yellow seed coat color formation in Brassica juncea and provided a comprehensive understanding of the yellow seed coat color mechanism.

Identification of a major QTL for silique length and seed weight in oilseed rape (Brassica napus L.)

2012 ◽  
Vol 125 (2) ◽  
pp. 285-296 ◽  
Author(s):  
Pu Yang ◽  
Chang Shu ◽  
Lin Chen ◽  
Jinsong Xu ◽  
Jiangsheng Wu ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Seed Weight ◽  
Brassica Napus L ◽  
Silique Length ◽  
Major Qtl

scholarly journals Targeted mutagenesis of BnTT8 homologs controls yellow seed coat development for effective oil production in Brassica napus L.

2020 ◽  
Vol 18 (5) ◽  
pp. 1153-1168 ◽  
Author(s):  
Yungu Zhai ◽  
Kaidi Yu ◽  
Shengli Cai ◽  
Limin Hu ◽  
Olalekan Amoo ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Seed Coat ◽  
Oil Production ◽  
Targeted Mutagenesis ◽  
Brassica Napus L ◽  
Yellow Seed ◽  
Yellow Seed Coat

scholarly journals Identification and QTL mapping of resistance to Turnip yellows virus (TuYV) in oilseed rape, Brassica napus

2019 ◽  
Vol 133 (2) ◽  
pp. 383-393 ◽  
Author(s):  
Dieter Hackenberg ◽  
Elvis Asare-Bediako ◽  
Adam Baker ◽  
Peter Walley ◽  
Carol Jenner ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Qtl Mapping ◽  
Oilseed Rape ◽  
Genetic Resource ◽  
Mapping Population ◽  
Resistant Parent ◽  
Potato Aphid ◽  
Turnip Yellows Virus ◽  
Peach Potato Aphid ◽  
Major Qtl

Abstract Key message Partially dominant resistance to Turnip yellows virus associated with one major QTL was identified in the natural allotetraploid oilseed rape cultivar Yudal. Abstract Turnip yellows virus (TuYV) is transmitted by the peach-potato aphid (Myzus persicae) and causes severe yield losses in commercial oilseed rape crops (Brassica napus). There is currently only one genetic resource for resistance to TuYV available in brassica, which was identified in the re-synthesised B. napus line ‘R54’. In our study, 27 mostly homozygous B. napus accessions, either doubled-haploid (DH) or inbred lines, representing a diverse subset of the B. napus genepool, were screened for TuYV resistance/susceptibility. Partial resistance to TuYV was identified in the Korean spring oilseed rape, B. napus variety Yudal, whilst the dwarf French winter oilseed rape line Darmor-bzh was susceptible. QTL mapping using the established Darmor-bzh × Yudal DH mapping population (DYDH) revealed one major QTL explaining 36% and 18% of the phenotypic variation in two independent experiments. A DYDH line was crossed to Yudal, and reciprocal backcross (BC1) populations from the F1 with either the susceptible or resistant parent revealed the dominant inheritance of the TuYV resistance. The QTL on ChrA04 was verified in the segregating BC1 population. A second minor QTL on ChrC05 was identified in one of the two DYDH experiments, and it was not observed in the BC1 population. The TuYV resistance QTL in ‘R54’ is within the QTL interval on Chr A04 of Yudal; however, the markers co-segregating with the ‘R54’ resistance are not conserved in Yudal, suggesting an independent origin of the TuYV resistances. This is the first report of the QTL mapping of TuYV resistance in natural B. napus.

scholarly journals Turnip mosaic virus determinants of virulence for Brassica napus resistance genes

2002 ◽  
Vol 38 (SI 1 - 6th Conf EFPP 2002) ◽  
pp. S155-S157
Author(s):  
C.E. Jenner ◽  
F. Sánchez ◽  
K. Tomimura ◽  
K. Ohshima ◽  
F. Ponz ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Mosaic Virus ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Infectious Clone ◽  
Turnip Mosaic Virus ◽  
Viral Fitness ◽  
Virulence Determinants ◽  
Similar Strategy ◽  
Napus Line

Dominant resistance genes identified in Brassica napus lines are effective against some, but not all, Turnip mosaic virus<br />(TuMV) isolates. An infectious clone of an isolate (UK 1) was used as the basis of chimeric virus constructions using<br />resistance-breaking mutants and other isolates to identify the virulence determinants for three dominant resistance genes.<br />For the resistance gene TuRB01, the presence of either of two mutations affecting the cylindrical inclusion (CI) protein<br />converted the avirulent UK 1 to a virulent isolate. Acquisition of such mutations had a slight cost to viral fitness in<br />plants lacking the resistance gene. A similar strategy is being used to identify the virulence determinants for two more<br />resistance genes present in another B. napus line.

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