Roles of Cch1 and Mid1 in Morphogenesis, Oxidative Stress Response and Virulence in Candida albicans

2012 ◽  
Vol 174 (5-6) ◽  
pp. 359-369 ◽  
Author(s):  
Qilin Yu ◽  
Hui Wang ◽  
Xinxin Cheng ◽  
Ning Xu ◽  
Xiaohui Ding ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Candida Albicans ◽  
Stress Response ◽  
Oxidative Stress Response

scholarly journals Trehalose accumulation induced during the oxidative stress response is independent of TPS1 mRNA levels in Candida albicans

2003 ◽  
Vol 6 (2) ◽  
pp. 121-125 ◽  
Author(s):  
�scar Zaragoza ◽  
Pilar Gonz�lez-P�rraga ◽  
Yolanda Pedre�o ◽  
Francisco J. Alvarez-Peral ◽  
Juan-Carlos Arg�elles
Keyword(s):  
Oxidative Stress ◽  
Candida Albicans ◽  
Stress Response ◽  
Oxidative Stress Response ◽  
Mrna Levels ◽  
Trehalose Accumulation

scholarly journals Deletion of the fungus specific protein phosphatase Z1 exaggerates the oxidative stress response in Candida albicans

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Krisztina Szabó ◽  
Ágnes Jakab ◽  
Szilárd Póliska ◽  
Katalin Petrényi ◽  
Katalin Kovács ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Candida Albicans ◽  
Stress Response ◽  
Protein Phosphatase ◽  
Transcript Level ◽  
Opportunistic Pathogen ◽  
Sublethal Concentration ◽  
Specific Protein ◽  
Oxidative Stress Response ◽  
Short Term Treatment

Abstract Background Candida albicans is an opportunistic pathogen which is responsible for widespread nosocomial infections. It encompasses a fungus specific serine/threonine protein phosphatase gene, CaPPZ1 that is involved in cation transport, cell wall integrity, oxidative stress response, morphological transition, and virulence according to the phenotypes of the cappz1 deletion mutant. Results We demonstrated that a short-term treatment with a sublethal concentration of tert-butyl hydroperoxide suppressed the growth of the fungal cells without affecting their viability, both in the cappz1 mutant and in the genetically matching QMY23 control strains. To reveal the gene expression changes behind the above observations we carried out a global transcriptome analysis. We used a pilot DNA microarray hybridization together with extensive RNA sequencing, and confirmed our results by quantitative RT-PCR. Novel functions of the CaPpz1 enzyme and oxidative stress mechanisms have been unraveled. The numbers of genes affected as well as the amplitudes of the transcript level changes indicated that the deletion of the phosphatase sensitized the response of C. albicans to oxidative stress conditions in important physiological functions like membrane transport, cell surface interactions, oxidation-reduction processes, translation and RNA metabolism. Conclusions We conclude that in the wild type C. albicans CaPPZ1 has a protective role against oxidative damage. We suggest that the specific inhibition of this phosphatase combined with mild oxidative treatment could be a feasible approach to topical antifungal therapy.

scholarly journals The Iron-Dependent Regulation of the Candida albicans Oxidative Stress Response by the CCAAT-Binding Factor

PLoS ONE ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. e0170649 ◽  
Author(s):  
Ananya Chakravarti ◽  
Kyle Camp ◽  
David S. McNabb ◽  
Inés Pinto
Keyword(s):  
Oxidative Stress ◽  
Candida Albicans ◽  
Stress Response ◽  
Oxidative Stress Response ◽  
Binding Factor

scholarly journals Candida albicans-Conditioned Medium Protects Yeast Cells from Oxidative Stress: a Possible Link between Quorum Sensing and Oxidative Stress Resistance

2005 ◽  
Vol 4 (10) ◽  
pp. 1654-1661 ◽  
Author(s):  
Caroline Westwater ◽  
Edward Balish ◽  
David A. Schofield
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Candida Albicans ◽  
Stress Response ◽  
Quorum Sensing ◽  
Conditioned Medium ◽  
Oxidative Stress Response ◽  
Yeast Cells ◽  
Protective Response ◽  
Quorum Sensing Molecule

ABSTRACT Candida albicans, the most frequent fungal pathogen of humans, encounters high levels of oxidants following ingestion by professional phagocytes and through contact with hydrogen peroxide-producing bacteria. In this study, we provide evidence that C. albicans is able to coordinately regulate the oxidative stress response at the global cell population level by releasing protective molecules into the surrounding medium. We demonstrate that conditioned medium, which is defined as a filter-sterilized supernatant from a C. albicans stationary-phase culture, is able to protect yeast cells from both hydrogen peroxide and superoxide anion-generating agents. Exponential-phase yeast cells preexposed to conditioned medium were able to survive levels of oxidative stress that would normally kill actively growing yeast cells. Heat treatment, digestion with proteinase K, pH adjustment, or the addition of the oxidant scavenger alpha-tocopherol did not alter the ability of conditioned medium to induce a protective response. Farnesol, a heat-stable quorum-sensing molecule (QSM) that is insensitive to proteolytic enzymes and is unaffected by pH extremes, is partly responsible for this protective response. In contrast, the QSM tyrosol did not alter the sensitivity of C. albicans cells to oxidants. Relative reverse transcription-PCR analysis indicates that Candida-conditioned growth medium induces the expression of CAT1, SOD1, SOD2, and SOD4, suggesting that protection may be mediated through the transcriptional regulation of antioxidant-encoding genes. Together, these data suggest a link between the quorum-sensing molecule farnesol and the oxidative stress response in C. albicans.

scholarly journals Niche-Specific Activation of the Oxidative Stress Response by the Pathogenic Fungus Candida albicans

2007 ◽  
Vol 75 (5) ◽  
pp. 2143-2151 ◽  
Author(s):  
Brice Enjalbert ◽  
Donna M. MacCallum ◽  
Frank C. Odds ◽  
Alistair J. P. Brown
Keyword(s):  
Oxidative Stress ◽  
Candida Albicans ◽  
Stress Response ◽  
Fluorescent Protein ◽  
Ex Vivo ◽  
Pathogenic Fungus ◽  
Systemic Infection ◽  
Oxidative Stress Response ◽  
Mammalian Host ◽  
Phagocytic Cells

ABSTRACT Candida albicans is a major opportunistic pathogen of humans. The pathogenicity of this fungus depends upon its ability to deal effectively with the host defenses and, in particular, the oxidative burst of phagocytic cells. We have explored the activation of the oxidative stress response in C. albicans in ex vivo infection models and during systemic infection of a mammalian host. We have generated C. albicans strains that contain specific green fluorescent protein (GFP) promoter fusions and hence act as biosensors of environmental oxidative stress at the single-cell level. Having confirmed that CTA1-, TRX1-, and TTR1/GRX2-GFP reporters respond specifically to oxidative stress, and not to heat shock, nitrosative, or osmotic stresses, we used these reporters to show that individual C. albicans cells activate an oxidative stress response following phagocytosis by neutrophils, but not by macrophages. Significantly, only a small proportion of C. albicans cells (about 4%) activated an oxidative stress response during systemic infection of the mouse kidney. The response of these cells was generally equivalent to exposure to 0.4 mM hydrogen peroxide in vitro. We conclude that most C. albicans cells are exposed to an oxidative stress when they come into contact with neutrophils in the bloodstream of the host but that oxidative killing is no longer a significant threat once an infection has been established in the kidney.

scholarly journals The Transcription Factor Sfp1 Regulates the Oxidative Stress Response in Candida albicans

2019 ◽  
Vol 7 (5) ◽  
pp. 131 ◽  
Author(s):  
Shao-Yu Lee ◽  
Hsueh-Fen Chen ◽  
Ying-Chieh Yeh ◽  
Yao-Peng Xue ◽  
Chung-Yu Lan
Keyword(s):  
Oxidative Stress ◽  
Transcription Factor ◽  
Candida Albicans ◽  
Stress Response ◽  
Oxidative Stress Response ◽  
Antifungal Drugs ◽  
Wild Type ◽  
Antioxidant Genes ◽  
Type C ◽  
Macrophage Killing

Candida albicans is a commensal that inhabits the skin and mucous membranes of humans. Because of the increasing immunocompromised population and the limited classes of antifungal drugs available, C. albicans has emerged as an important opportunistic pathogen with high mortality rates. During infection and therapy, C. albicans frequently encounters immune cells and antifungal drugs, many of which exert their antimicrobial activity by inducing the production of reactive oxygen species (ROS). Therefore, antioxidative capacity is important for the survival and pathogenesis of C. albicans. In this study, we characterized the roles of the zinc finger transcription factor Sfp1 in the oxidative stress response against C. albicans. A sfp1-deleted mutant was more resistant to oxidants and macrophage killing than wild-type C. albicans and processed an active oxidative stress response with the phosphorylation of the mitogen-activated protein kinase (MAPK) Hog1 and high CAP1 expression. Moreover, the sfp1-deleted mutant exhibited high expression levels of antioxidant genes in response to oxidative stress, resulting in a higher total antioxidant capacity, glutathione content, and glutathione peroxidase and superoxide dismutase enzyme activity than the wild-type C. albicans. Finally, the sfp1-deleted mutant was resistant to macrophage killing and ROS-generating antifungal drugs. Together, our findings provide a new understanding of the complex regulatory machinery in the C. albicans oxidative stress response.

The Calcium Channel Blocker Verapamil Inhibits Oxidative Stress Response in Candida albicans

2014 ◽  
Vol 177 (3-4) ◽  
pp. 167-177 ◽  
Author(s):  
Qilin Yu ◽  
Chenpeng Xiao ◽  
Kailun Zhang ◽  
Chang Jia ◽  
Xiaohui Ding ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Candida Albicans ◽  
Stress Response ◽  
Calcium Channel ◽  
Calcium Channel Blocker ◽  
Channel Blocker ◽  
Oxidative Stress Response

The Sko1 protein represses the yeast-to-hypha transition and regulates the oxidative stress response in Candida albicans

2010 ◽  
Vol 47 (7) ◽  
pp. 587-601 ◽  
Author(s):  
Rebeca Alonso-Monge ◽  
Elvira Román ◽  
David M. Arana ◽  
Daniel Prieto ◽  
Verónica Urrialde ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Candida Albicans ◽  
Stress Response ◽  
Oxidative Stress Response
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