TiO2 nanoparticles alleviate toxicity by reducing free Zn2+ ion in human primary epidermal keratinocytes exposed to ZnO nanoparticles

<p>Present paper relates to nanomaterials based decontamination formulation made up of TiO2, MgO, and ZnO nanoparticles for the use against chemical warfare agents. This decontamination formulation was prepared by mixing 90 per cent of TiO2 nanoparticles of 5-15 nm size range, 8 per cent of MgO nanoparticles of 5-15 nm size range, and 2 per cent of ZnO nanoparticles of 20-30 nm size range. Prepared formulation exhibited 98-99 per cent of physical removal efficiency against contaminated glass, rubber, painted metal, metal surfaces. It efficiently removed chemical warfare agents from contaminated skin thus ameliorating chances of fatality which is expected due to skin penetrated chemical warfare agents. It also chemically degraded 97 per cent of sulphur mustard in 24 h, 99.9 per cent of sarin in 2 h, respectively unlike Fuller’s earth which chemically degraded only 63 per cent of sulphur mustard and 59 per cent of sarin in 24 h .</p>

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Effects of Orthodontic Bonding Containing TiO2 and ZnO Nanoparticles on Prevention of White Spot Lesions: an In Vitro Study

Biointerface Research in Applied Chemistry ◽

10.33263/briac121.431440 ◽

2021 ◽

Vol 12 (1) ◽

pp. 431-440

Keyword(s):

Tio2 Nanoparticles ◽

Zno Nanoparticles ◽

Brain Heart Infusion ◽

Negative Control ◽

White Spot ◽

Control Group ◽

P Value ◽

White Spot Lesions ◽

White Spots

Demineralization is a common problem following orthodontic treatments. Today using antibacterial nanoparticles in preventing white spot lesions is being discussed. Given that ZnO and TiO2 nanoparticles have direct antibacterial known properties, this study aims to evaluate these nanoparticles' antibacterial effects in orthodontic bondings' composition on preventing white spot lesions. In this in vitro experimental study, 43 sound human premolar teeth were divided into five groups according to the adhesive utilized for bracket bonding: None group consisting of 12 teeth bonded with Transbond XT, TiO2 Group consisting of 12 teeth with Transbond XT and TiO2 nanoparticles, ZnO group consisting of 12 teeth with Transbond XT and ZnO nanoparticles, a positive control group consisting of 5 teeth without brackets and negative control groups consisting of 5 teeth in a sterile medium. All teeth were stored in a medium consisting of 1cc brain heart infusion (BHI) + sucrose 1%+ 0.5 McFarland Streptococcus mutans bacteria for 28 days. The medium was replaced every 48 hours. All the samples were examined every week for 4 weeks using DIAGNOdent and photography to detect white spots. The results of this study revealed that adding TiO2 and ZnO nanoparticles to Transbond XT bonding caused a decrease in enamel lesions occurrence and incidence of white spots (p value= 0.00). The results did not reveal significant differences between TiO2 and ZnO groups. Novel bonding agents containing TiO2 and ZnO nanoparticles represent promising candidates in combating enamel white spot lesions.

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Diminished inhibitory impact of ZnO nanoparticles on anaerobic fermentation by the presence of TiO2 nanoparticles: Phenomenon and mechanism

The Science of The Total Environment ◽

10.1016/j.scitotenv.2018.07.468 ◽

2019 ◽

Vol 647 ◽

pp. 313-322 ◽

Cited By ~ 8

Author(s):

Lingling Zhang ◽

Zhaoxi Zhang ◽

Xi He ◽

Lei Zheng ◽

Shikun Cheng ◽

...

Keyword(s):

Tio2 Nanoparticles ◽

Zno Nanoparticles ◽

Anaerobic Fermentation

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Alterations of ultrastructure and elemental composition in cultured human epidermal keratinocytes after treatment with nitrofurazone and silver sulfadiazine

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100127803 ◽

1987 ◽

Vol 45 ◽

pp. 676-677

Author(s):

A. R. Crooker ◽

M. C. Myers ◽

T. L. Beard ◽

E. S. Graham

Keyword(s):

Electron Microscopy ◽

Elemental Composition ◽

Pyrolytic Carbon ◽

Human Keratinocytes ◽

Silver Sulfadiazine ◽

Epidermal Keratinocytes ◽

Human Epidermal Keratinocytes ◽

Culture Systems ◽

Cytotoxicity Endpoints

Cell culture systems have become increasingly popular as a means of screening toxic agents and studying toxic mechanisms of drugs and other chemicals at the cellular and subcellular levels. These in vitro tests can be conducted rapidly in a broad range of relevant mammalian culture systems; a variety of biological and biochemical cytotoxicity endpoints can be examined. The following study utilized human keratinocytes to evaluate the relative cytotoxicities of nitrofurazone (NF) and silver sulfadiazine (SS), the active ingredients of FURACIN(R) Topical Cream and SILVADENE(R) Cream, respectively. These compounds are anti-infectives used in the treatment of burn patients. Cell ultrastructure and elemental composition were utilized as cytotoxicity endpoints.Normal Human Epidermal Keratinocytes (HK) were prepared from the EpiPackTM culture system (Clonetics Corporation, Boulder, CO). For scanning electron microscopy (SEM) and transmission electron microscopy (TEM), cells were seeded on sterile 35 mm Falcon plastic dishes; for elemental microanalysis, cells were plated on polished pyrolytic carbon discs (E. Fullam, Latham, NY) placed in the culture dishes.

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