scholarly journals Aptamer-conjugated multi-walled carbon nanotubes as a new targeted ultrasound contrast agent for the diagnosis of prostate cancer

2018 ◽  
Vol 20 (11) ◽  
Author(s):  
Fenfen Gu ◽  
Chuling Hu ◽  
Qingming Xia ◽  
Chunai Gong ◽  
Shen Gao ◽  
...  
2009 ◽  
Vol 31 (4) ◽  
pp. 235-246 ◽  
Author(s):  
Szu-Chia Chen ◽  
Jia-Ling Ruan ◽  
Po-Wen Cheng ◽  
Yueh-Hsun Chuang ◽  
Pai-Chi Li

A thrombus-targeted ultrasound contrast agent bound with tirofiban — a glycoprotein (GP) IIb/IIIa antagonist that can specifically bind to activated platelets in the thrombus — was designed to enhance both the image contrast and thrombolysis effect. In this study, we used 76 canine thrombi for investigation. The targeting ability to thrombi was confirmed by microphotography and high-frequency ultrasound (40 MHz) imaging. The effect of the targeted microbubbles on thrombolysis enhancement was investigated using an in vitro flow system: targeted and nontargeted microbubbles flowed through the clot for 30 seconds with a washing step; the microbubbles remained on the clot that were then cavitated by ultrasound (frequency = 1 MHz, MI = 1.2). The extent of thrombolysis was evaluated by weight reduction and histology analysis. The targeted microbubbles reduced the weight of thrombi by a factor of 1.7 times that of the nontargeted microbubbles. (clot weight reduction: 23.1 ± 5.3% versus 13.6 ± 4.9%, p < 0.01 between targeted and nontargeted group), and the signal enhancement was 3.34 ± 0.30 dB (mean ± SD, p < 0.01 compared to control). We conclude that targeted microbubbles are applicable not only for molecular imaging of thrombi but also for improving the effectiveness of ultrasound-assisted thrombolysis.


2021 ◽  
Author(s):  
Reza Alisani ◽  
Navid Rakhshani ◽  
maryam Abolhallaj ◽  
Foojan Motevallid ◽  
Parvaneh Ghaderi-shekhi Abadi ◽  
...  

Abstract The cellulose acetate (CA)/poly (ε-caprolactone diol)/poly (tetramethylene ether) glycol-polyurethane (PCL-Diol/PTMG-PU)/multi-walled carbon nanotubes (MWCNTs) composite nanofibers were prepared via two-nozzle electrospinning on both counter sides of the collector. The performance of synthesized composite nanofibers was investigated as an environmental application and anticancer delivery system for the adsorption/release of doxorubicin (DOX). The synergic effect of MWCNTs and DOX incorporated into the nanofibers was investigated against LNCaP prostate cancer cells. The status of MWCNTs and DOX in composite nanofibers was demonstrated by SEM, FTIR and UV-Vis determinations. The adsorption tests using nanofibrous adsorbent toward DOX sorption was evaluated under various DOX initial concentrations (100-2000 mgL-1 ), adsorption times (5-120 minutes), and pH values (pH:2-9). Due to the fitting of isotherm and kinetic data with Redlich-Peterson and pseudo-second order models, both chemisorption and surface adsorption of DOX molecules mechanisms have been predicted. The drug release from both nanofibers and MWCNTs-loaded nanofibers was compared. The better drug sustained release profiles verified in the presence of composite nanofibers. LNCaP prostate cancer and L929 normal cells were treated to investigate the cytotoxicity and compatibility of synthesized composite nanofibers. The apoptosis/necrosis of hybrid nanofibers and MWCNTs loaded-nanofibers was investigated. The obtained results demonstrated the synergic effects of MWCNTs and DOX loaded-nanofibers on the LNCaP prostate cancer cells death.


PLoS ONE ◽  
2016 ◽  
Vol 11 (2) ◽  
pp. e0149075 ◽  
Author(s):  
Qiao Hu ◽  
Xiao-Yan Wang ◽  
Li-Ke Kang ◽  
Hai-Ming Wei ◽  
Chun-Mei Xu ◽  
...  

Tumor Biology ◽  
2017 ◽  
Vol 39 (3) ◽  
pp. 101042831769594 ◽  
Author(s):  
Seung S Lee ◽  
Philip JR Roche ◽  
Paresa N Giannopoulos ◽  
Elliot J Mitmaker ◽  
Michael Tamilia ◽  
...  

Almost all biological therapeutic interventions cannot overcome neoplastic heterogeneity. Physical ablation therapy is immune to tumor heterogeneity, but nearby tissue damage is the limiting factor in delivering lethal doses. Multi-walled carbon nanotubes offer a number of unique properties: chemical stability, photonic properties including efficient light absorption, thermal conductivity, and extensive surface area availability for covalent chemical ligation. When combined together with a targeting moiety such as an antibody or small molecule, one can deliver highly localized temperature increases and cause extensive cellular damage. We have functionalized multi-walled carbon nanotubes by conjugating an antibody against prostate-specific membrane antigen. In our in vitro studies using prostate-specific membrane antigen–positive LNCaP prostate cancer cells, we have effectively demonstrated cell ablation of >80% with a single 30-s exposure to a 2.7-W, 532-nm laser for the first time without bulk heating. We also confirmed the specificity and selectivity of prostate-specific membrane antigen targeting by assessing prostate-specific membrane antigen–null PC3 cell lines under the same conditions (<10% cell ablation). This suggests that we can achieve an extreme nearfield cell ablation effect, thus restricting potential tissue damage when transferred to in vivo clinical applications. Developing this new platform will introduce novel approaches toward current therapeutic modalities and will usher in a new age of effective cancer treatment squarely addressing tumoral heterogeneity.


2011 ◽  
Vol 56 (19) ◽  
pp. 6161-6177 ◽  
Author(s):  
Valeria Garbin ◽  
Marlies Overvelde ◽  
Benjamin Dollet ◽  
Nico de Jong ◽  
Detlef Lohse ◽  
...  

RSC Advances ◽  
2020 ◽  
Vol 10 (63) ◽  
pp. 38592-38600 ◽  
Author(s):  
Jie Zhang ◽  
Jinzi Yang ◽  
Huiming Zhang ◽  
Ming Hu ◽  
Jinjing Li ◽  
...  

By attaching ferroferric oxide (Fe3O4) to drug-carrying carbon nanotubes (CNTs), we generated a new Span-PEG composite with Fe3O4-CNT multifunctional microbubbles for inflammation and thrombus niduses.


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