Neuroprotective Effect of Melatonin Against PCBs Induced Behavioural, Molecular and Histological Changes in Cerebral Cortex of Adult Male Wistar Rats

The biochemical and histological changes following 60 days administration of daily doses equivalent to 1/20 LD50 of lithium lactate and hydrochlorothiazide, as such and in association, were studied in male Wistar rats. No mortality or overt signs of toxicity were observed during the experiment and the serum activities of transaminases, alkaline phosphatase and cholinesterase were not significantly modified compared to controls. The histopathological examination of all the investigated organs: kidney, liver, brain and spleen, revealed significant lesions which were time-dependant and more pronounced in the association group. Although the changes were mostly inflammatory and conqestive, it was proved that the concomitant administration of lithium and hydrochlorothiazid is potentially dangerous, increasing lithium’s nephrotoxicity and the thiazide diuretic's hepatotoxicity.

Download Full-text

Anti‐Fertility Effects of Amlodipine in Adult Male Wistar Rats

The FASEB Journal ◽

10.1096/fasebj.25.1_supplement.873.3 ◽

2011 ◽

Vol 25 (S1) ◽

Author(s):

Adelaja Abdulazeez Akinlolu ◽

Olaide Ghazali ◽

Adebayo Kehinde Adefule

Keyword(s):

Adult Male ◽

Wistar Rats ◽

Male Wistar Rats

Download Full-text

Azathioprine and Methotrexate impaired the morphology and functions of the testes in adult wistar rats

Journal of Morphological Sciences ◽

10.4322/jms.057513 ◽

2014 ◽

Vol 31 (02) ◽

pp. 075-081

Author(s):

A. Akinlolu ◽

O. Akinola ◽

P. Khobe ◽

K. Obasi ◽

O. Dada

Keyword(s):

Adult Male ◽

Wistar Rats ◽

Follicle Stimulating Hormone ◽

Physiological Saline ◽

Seminiferous Tubules ◽

Control Group ◽

Connective Tissues ◽

Group I ◽

Male Wistar Rats ◽

Dose Dependent

Abstract Introduction: AAzathioprine and Methotrexate are both used in the treatment of cancer; and are classified as cytotoxic drugs with reported adverse effects such as oxidative damage to the DNA/RNA, the testes and sperm cells. This study, therefore, tested the hypothesis that AAzathioprine and Methotrexate administrations impair the morphology and functions of the testes in adult male wistar rats. Methods: AAzathioprine (50-150mg per day) and Methotrexate (2.5mg per week) are used in the treatment of cancer in adult Man. We tested the hypothesis that AAzathioprine and Methotrexate impair the morphology and functions of testes in rats. Forty adult male wistar rats (150-230g) were employed in the study: Control Group I received physiological saline while Experimental Groups II - V received oral administrations of 5mg/kg/bodyweight of AAzathioprine per day, 15mg/kg/bodyweight of AAzathioprine per day, 8mg/kg/bodyweight of Methotrexate per week and 20mg/kg/bodyweight of Methotrexate per week respectively for 35 days. Results: Histological examinations of the testes of rats of Groups II - V showed dose-dependent morphological anomalies such as fewer collagen ibers of connective tissues, disrupted seminiferous tubules and scanty spermatozoa when compared to rats of Group I. Statistical analyses showed dose-dependent elevated levels (P≤0.05) of superoxide dismutase and malondialdehyde in testes homogenates of rats of Groups II - V when compared to rats of Group I. This implied increased oxidative stress in rats of Groups II - V. Evaluations of Follicle Stimulating Hormone and Testosterone showed dose-dependent significantly elevated levels (P≤0.05) in rats of Groups II - V when compared to rats of Group I. Conclusions: Our findings are consistent with the stated hypothesis.

Download Full-text

Avoidance memory requires CaMKII activity to persist after recall

Molecular Brain ◽

10.1186/s13041-021-00877-5 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Andressa Radiske ◽

Maria Carolina Gonzalez ◽

Janine I. Rossato ◽

Gênedy Apolinário ◽

João R. de Oliveira ◽

...

Keyword(s):

Protein Kinase ◽

Adult Male ◽

Wistar Rats ◽

Memory Reconsolidation ◽

Memory Processing ◽

Conflicting Information ◽

Male Wistar Rats ◽

Avoidance Memory ◽

Avoidance Responses

AbstractAvoidance memory is destabilized when recalled concurrently with conflicting information, and must undergo a hippocampus-dependent restabilization process called reconsolidation to persist. CaMKII is a serine/threonine protein kinase essential for memory processing; however, its possible involvement in avoidance memory reconsolidation has not yet been studied. Using pharmacological, electrophysiological and optogenetic tools, we found that in adult male Wistar rats hippocampal CaMKII is necessary to reconsolidate avoidance memory, but not to keep it stored while inactive, and that blocking reconsolidation via CaMKII inhibition erases learned avoidance responses.

Download Full-text