Effects of Antigen-Expressing Immunostimulatory Liposomes on Chemotaxis and Maturation of Dendritic Cells In Vitro and in Human Skin Explants

2013 ◽  
Vol 31 (2) ◽  
pp. 516-526 ◽  
Author(s):  
Anastasia Lanzi ◽  
Cynthia M. Fehres ◽  
Tanja D. de Gruijl ◽  
Yvette van Kooyk ◽  
Enrico Mastrobattista
Keyword(s):  
Dendritic Cells ◽  
Human Skin ◽  
Skin Explants

scholarly journals HIV-1 Selection by Epidermal Dendritic Cells during Transmission across Human Skin

1998 ◽  
Vol 187 (10) ◽  
pp. 1623-1631 ◽  
Author(s):  
Jeanette C. Reece ◽  
Amanda J. Handley ◽  
E. John Anstee ◽  
Wayne A. Morrison ◽  
Suzanne M. Crowe ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Human Skin ◽  
Cell Sorting ◽  
Virus Entry ◽  
Skin Explants ◽  
Skin Uptake ◽  
Virus Exposure ◽  
Epidermal Dendritic Cells ◽  
Hiv 1

Macrophage tropic HIV-1 is predominant during the initial viremia after person to person transmission of HIV-1 (Zhu, T., H. Mo, N. Wang, D.S. Nam, Y. Cao, R.A. Koup, and D.D. Ho. 1993. Science. 261:1179–1181.), and this selection may occur during virus entry and carriage to the lymphoid tissue. Human skin explants were used to model HIV-1 selection that may occur at the skin or mucosal surface. Macrophage tropic, but not T cell line tropic strains of HIV-1 applied to the abraded epidermis were recovered from the cells emigrating from the skin explants. Dermis and epidermis were separated by dispase digestion after virus exposure to determine the site of viral selection within the skin. Uptake and transmission to T cells of all HIV-1 isolates was found with the dermal emigrant cells, but only macrophage tropic virus was transferred by emigrants from the epidermis exposed to HIV-1, indicating selection only within the epidermis. CD3+, CD4+ T cells were found in both the dermal and epidermal emigrant cells. After cell sorting to exclude contaminating T cells, macrophage tropic HIV-1 was found in both the dermal emigrant dendritic cells and in dendritic cells sorted from the epidermal emigrants. These observations suggest that selective infection of the immature epidermal dendritic cells represents the cellular mechanism that limits the initial viremia to HIV-1 that can use the CCR5 coreceptor.

Assessment of the biochemical effects of percutaneous exposure of sulphur mustard in an in vitro human skin system

1996 ◽  
Vol 15 (3) ◽  
pp. 237-244 ◽  
Author(s):  
CD Lindsay ◽  
P. Rice
Keyword(s):  
Inflammatory Response ◽  
Human Skin ◽  
Serine Proteases ◽  
Inflammatory Cells ◽  
Chemical Warfare Agent ◽  
Skin Damage ◽  
Sulphur Mustard ◽  
Nuclear Pyknosis ◽  
Skin Explants

1 Sulphur mustard (HD) is a potent chemical warfare agent which causes incapacitating blisters on human skin. There is no specific pretreatment nor therapy against this agent and the mechanism of dermo-epidermal cleavage is unclear. The aim of this study was to use a human skin explant system to determine the consequences of percuta neous exposure to HD. 2 Increased activities of serine proteases associated with blistering disorders in humans were detected from human skin explants after exposure to HD. The most consistent response and the highest protease activities measured were found for trypsin. This class of enzyme is therefore implicated in the dermo-epidermal separation which is associated with blistering in humans following exposure to HD. 3 An inflammatory response was observed in the skin explants exposed to HD. At low doses of HD it was characterised by the presence of neutrophils in the papillary dermis, culminating in the infiltration of the epidermis by these inflammatory cells at higher concen trations of HD. A variety of other histopathological changes in the explants was found such as focal dermo- epidermal separation, nuclear pyknosis and perinuclear vacuolation. 4 The study indicates that full thickness human skin explants can be used to investigate various aspects of the possible pathogenesis of HD-induced skin damage, in cluding the associated inflammatory response.

scholarly journals Prolonged Maturation and Enhanced Transduction of Dendritic Cells Migrated from Human Skin Explants After In Situ Delivery of CD40-Targeted Adenoviral Vectors

2002 ◽  
Vol 169 (9) ◽  
pp. 5322-5331 ◽  
Author(s):  
Tanja D. de Gruijl ◽  
Sylvia A. Luykx-de Bakker ◽  
Bryan W. Tillman ◽  
Alfons J. M. van den Eertwegh ◽  
Jan Buter ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Human Skin ◽  
Adenoviral Vectors ◽  
Skin Explants

A putative in vitro organotypic model of molting with human skin explants

2011 ◽  
Vol 304 (2) ◽  
pp. 145-153 ◽  
Author(s):  
A. Peramo ◽  
S. E. Feinberg ◽  
C. L. Marcelo
Keyword(s):  
Human Skin ◽  
Skin Explants

Comparison of the metabolism of 10 cosmetics‐relevant chemicals in EpiSkin™ S9 subcellular fractions and in vitro human skin explants

2019 ◽  
Vol 40 (2) ◽  
pp. 313-326 ◽  
Author(s):  
Camille Géniès ◽  
Carine Jacques‐Jamin ◽  
Hélène Duplan ◽  
Helga Rothe ◽  
Corie Ellison ◽  
...  
Keyword(s):  
Human Skin ◽  
Subcellular Fractions ◽  
Skin Explants

Visible effects of rapamycin (sirolimus) on human skin explants in vitro

2012 ◽  
Vol 305 (2) ◽  
pp. 163-171 ◽  
Author(s):  
Antonio Peramo ◽  
Cynthia L. Marcelo
Keyword(s):  
Human Skin ◽  
Skin Explants

scholarly journals Activin A Induces Langerhans Cell Differentiation In Vitro and in Human Skin Explants

PLoS ONE ◽  
2008 ◽  
Vol 3 (9) ◽  
pp. e3271 ◽  
Author(s):  
Tiziana Musso ◽  
Sara Scutera ◽  
William Vermi ◽  
Roberta Daniele ◽  
Michele Fornaro ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Human Skin ◽  
Activin A ◽  
Langerhans Cell ◽  
Skin Explants

scholarly journals Antioxidant and Anti-Aging Potential of Indian Sandalwood Oil against Environmental Stressors In Vitro and Ex Vivo

Cosmetics ◽  
2021 ◽  
Vol 8 (2) ◽  
pp. 53
Author(s):  
Véronique Francois-Newton ◽  
Andrew Brown ◽  
Philippe Andres ◽  
Madiiha Bibi Mandary ◽  
Carli Weyers ◽  
...  
Keyword(s):  
Human Skin ◽  
Active Ingredient ◽  
Ex Vivo ◽  
Environmental Stressors ◽  
Alpha Tocopherol ◽  
Santalum Album ◽  
Aging Effects ◽  
Sandalwood Oil ◽  
Skin Explants

Distilled from the heartwood of Santalum album, Indian sandalwood oil is an essential oil that historically has been used as a natural active ingredient in cosmetics to condition and brighten the skin. It has been documented to exhibit antioxidant, anti-inflammatory, and anti-proliferative activities. Here, we investigated the protective and anti-aging effects of Indian sandalwood oil in scavenging reactive oxygen species (ROS) in HaCaT cells and in human skin explants after exposure to oxidative stress. Using a probe DCFH-DA, the antioxidant capacity of Indian sandalwood oil was monitored following exposure to blue light at 412 nm and 450 nm or cigarette smoke. The anti-aging effect of sandalwood oil was also explored in human skin explants via the assessment of collagenase level (MMP-1). We reported that Indian sandalwood oil possessed antioxidant potential that can scavenge the ROS generated by a free radical generating compound (AAPH). Subsequent exposure to environmental stressors revealed that Indian sandalwood oil possessed superior antioxidant activity in comparison to vitamin E (alpha tocopherol). Using human skin explants, this study demonstrated that Indian sandalwood oil can also inhibit the pollutant-induced level of MMP-1. The findings indicated that Indian sandalwood oil can potentially serve as a protective and anti-aging active ingredient in cosmetics and dermatology against environmental stressors.

Percutaneous Biomedical Device with a Regenerative Materials Interface

2008 ◽  
Vol 1136 ◽  
Author(s):  
Antonio Peramo ◽  
Cynthia L. Marcelo ◽  
Steve Goldstein ◽  
David C. Martin
Keyword(s):  
Stainless Steel ◽  
Antimicrobial Peptides ◽  
Human Skin ◽  
Culture System ◽  
Local Administration ◽  
Junctional Epithelium ◽  
Biomedical Device ◽  
Skin Explants

ABSTRACTWe have developed an in vitro culture system composed of organotypic human skin explants interfaced with titanium rods or stainless steel fixator pins. The use of this interface provides a model to evaluate strategies for creating a stable, long-term connection with living skin and chronic percutaneous devices. Our hypothesis is that the delivery of specific biomaterials at this interface will create a dynamic, slowly flowing matrix for skin biointegration, local administration of drugs or antimicrobials. We define this concept as the generation of an artificial mucosa, because it mimics the situation of several epithelial tissues (like the periodontal junction between the tooth and the junctional epithelium) where antimicrobial peptides and mucins are constantly extruded.

Sign in / Sign up

Export Citation Format

Share Document