Comparison of the metabolism of 10 cosmetics‐relevant chemicals in EpiSkin™ S9 subcellular fractions and in vitro human skin explants

2019 ◽  
Vol 40 (2) ◽  
pp. 313-326 ◽  
Author(s):  
Camille Géniès ◽  
Carine Jacques‐Jamin ◽  
Hélène Duplan ◽  
Helga Rothe ◽  
Corie Ellison ◽  
...  
1996 ◽  
Vol 15 (3) ◽  
pp. 237-244 ◽  
Author(s):  
CD Lindsay ◽  
P. Rice

1 Sulphur mustard (HD) is a potent chemical warfare agent which causes incapacitating blisters on human skin. There is no specific pretreatment nor therapy against this agent and the mechanism of dermo-epidermal cleavage is unclear. The aim of this study was to use a human skin explant system to determine the consequences of percuta neous exposure to HD. 2 Increased activities of serine proteases associated with blistering disorders in humans were detected from human skin explants after exposure to HD. The most consistent response and the highest protease activities measured were found for trypsin. This class of enzyme is therefore implicated in the dermo-epidermal separation which is associated with blistering in humans following exposure to HD. 3 An inflammatory response was observed in the skin explants exposed to HD. At low doses of HD it was characterised by the presence of neutrophils in the papillary dermis, culminating in the infiltration of the epidermis by these inflammatory cells at higher concen trations of HD. A variety of other histopathological changes in the explants was found such as focal dermo- epidermal separation, nuclear pyknosis and perinuclear vacuolation. 4 The study indicates that full thickness human skin explants can be used to investigate various aspects of the possible pathogenesis of HD-induced skin damage, in cluding the associated inflammatory response.


2011 ◽  
Vol 304 (2) ◽  
pp. 145-153 ◽  
Author(s):  
A. Peramo ◽  
S. E. Feinberg ◽  
C. L. Marcelo
Keyword(s):  

2012 ◽  
Vol 305 (2) ◽  
pp. 163-171 ◽  
Author(s):  
Antonio Peramo ◽  
Cynthia L. Marcelo
Keyword(s):  

PLoS ONE ◽  
2008 ◽  
Vol 3 (9) ◽  
pp. e3271 ◽  
Author(s):  
Tiziana Musso ◽  
Sara Scutera ◽  
William Vermi ◽  
Roberta Daniele ◽  
Michele Fornaro ◽  
...  

Cosmetics ◽  
2021 ◽  
Vol 8 (2) ◽  
pp. 53
Author(s):  
Véronique Francois-Newton ◽  
Andrew Brown ◽  
Philippe Andres ◽  
Madiiha Bibi Mandary ◽  
Carli Weyers ◽  
...  

Distilled from the heartwood of Santalum album, Indian sandalwood oil is an essential oil that historically has been used as a natural active ingredient in cosmetics to condition and brighten the skin. It has been documented to exhibit antioxidant, anti-inflammatory, and anti-proliferative activities. Here, we investigated the protective and anti-aging effects of Indian sandalwood oil in scavenging reactive oxygen species (ROS) in HaCaT cells and in human skin explants after exposure to oxidative stress. Using a probe DCFH-DA, the antioxidant capacity of Indian sandalwood oil was monitored following exposure to blue light at 412 nm and 450 nm or cigarette smoke. The anti-aging effect of sandalwood oil was also explored in human skin explants via the assessment of collagenase level (MMP-1). We reported that Indian sandalwood oil possessed antioxidant potential that can scavenge the ROS generated by a free radical generating compound (AAPH). Subsequent exposure to environmental stressors revealed that Indian sandalwood oil possessed superior antioxidant activity in comparison to vitamin E (alpha tocopherol). Using human skin explants, this study demonstrated that Indian sandalwood oil can also inhibit the pollutant-induced level of MMP-1. The findings indicated that Indian sandalwood oil can potentially serve as a protective and anti-aging active ingredient in cosmetics and dermatology against environmental stressors.


2008 ◽  
Vol 1136 ◽  
Author(s):  
Antonio Peramo ◽  
Cynthia L. Marcelo ◽  
Steve Goldstein ◽  
David C. Martin

ABSTRACTWe have developed an in vitro culture system composed of organotypic human skin explants interfaced with titanium rods or stainless steel fixator pins. The use of this interface provides a model to evaluate strategies for creating a stable, long-term connection with living skin and chronic percutaneous devices. Our hypothesis is that the delivery of specific biomaterials at this interface will create a dynamic, slowly flowing matrix for skin biointegration, local administration of drugs or antimicrobials. We define this concept as the generation of an artificial mucosa, because it mimics the situation of several epithelial tissues (like the periodontal junction between the tooth and the junctional epithelium) where antimicrobial peptides and mucins are constantly extruded.


2013 ◽  
Vol 31 (2) ◽  
pp. 516-526 ◽  
Author(s):  
Anastasia Lanzi ◽  
Cynthia M. Fehres ◽  
Tanja D. de Gruijl ◽  
Yvette van Kooyk ◽  
Enrico Mastrobattista

Author(s):  
Ronni Wolf ◽  
Mariarosaria D'Avino ◽  
Francesca De Angelis ◽  
Eleonora Ruocco ◽  
Maria Luisa Lombardi

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