scholarly journals Advanced analysis of oligomeric proanthocyanidins: latest approaches in liquid chromatography and mass spectrometry based analysis

2021 ◽  
Author(s):  
Nico Symma ◽  
Andreas Hensel
Keyword(s):  
Mass Spectrometry ◽  
Analytical Techniques ◽  
Partial Hydrolysis ◽  
Innovative Strategies ◽  
Oligomeric Proanthocyanidins ◽  
Maldi Tof ◽  
Advanced Analysis ◽  
Kendrick Mass Defect ◽  
Kendrick Mass Defect Analysis ◽  
Stop Flow

AbstractProanthocyanidins (PAC) are an important and widely spread class of natural products with various bioactivities. The analytical evaluation of oligomeric and polymeric proanthocyanidins in complex extracts is still challenging, due to the complexity of structures. Nevertheless, in the last 20 years analytical techniques especially in the field of chromatography and mass spectrometry have been improved and offer exciting possibilities for a deeper insight into plant secondary metabolism. While classical colorimetric methods and protocols for partial hydrolysis are still valuable tools new chromatographically and mass spectrometric methods provide deep insights into the PAC fine structure, also of polymeric material. The present review focus on recent innovative LC–MS and MALDI-TOF methods, innovative strategies for advanced processing of LC-HRMS data (van Krevelen plots, Kendrick Mass Defect Analysis), MS/MS fragmentation, and newest two-dimensional LC × LC (Offline, Online, Stop-flow-2D) chromatography. Also MALDI-TOF imaging provides exciting inside in PAC distribution within biological samples and functional assays.

Automated High Throughput Multiple Target Screening of Molecular Libraries by Microfluidic MALDI-TOF MS

1998 ◽  
Vol 3 (3) ◽  
pp. 189-198 ◽  
Author(s):  
Frank Hsieh ◽  
Hasmik Keshishian ◽  
Craig Muir
Keyword(s):  
Mass Spectrometry ◽  
Enzyme Inhibitors ◽  
Analytical Techniques ◽  
Multiple Target ◽  
Maldi Tof Ms ◽  
Lead Compounds ◽  
Maldi Tof ◽  
Target Screening ◽  
Molecular Libraries ◽  
Tof Ms

Novel analytical techniques are demanded in parallel in the automated combinatorial library syntheses for accelerating the process of drug discovery. In this study, the integration of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and robotics for simultaneously identifying lead compounds with activity against multiple enzyme targets has been shown. MALDI-TOF MS monitors the interactions between multienzyme targets and a library of compounds and then identifies individual compounds from molecular libraries that affect the enzymatic activities of multiplexed target molecules to catalyze the conversion of substrates to products. The novel mass spectrometry screening in high-density format (~4,000 samples in a single 4.5 × 4.5 cm MALDI plate) provides much higher throughput over traditional screening approaches in terms of multiplex targets, attomole-level sensitivity, very low volume of samples required (10−9−10−12 1), and data acquisition for each sample within ten sec. The microfluidic multiple target screening approach mass spectrometry was shown for discovery of enzyme inhibitors as potential lead compounds.

Chicken Pituitary Glycoproteins: New Isolation Method

1999 ◽  
Vol 64 (9) ◽  
pp. 1510-1516
Author(s):  
Helena Ryšlavá ◽  
Jana Krešlová ◽  
Jana Barthová ◽  
Tomislav Barth
Keyword(s):  
Mass Spectrometry ◽  
Luteinizing Hormone ◽  
New Method ◽  
Hormonal Activity ◽  
Isolation Method ◽  
Molecular Weights ◽  
Testosterone Production ◽  
Maldi Tof ◽  
Tryptic Cleavage

A new method for isolation of glycoproteins from chicken pituitaries was applied. The procedure consist of chromatography on ConA-Sepharose and by HPLC on S Hyper D and Vydac C4 columns. The hormonal activity of the glycoproteins was tested by determining their stimulatory effect on cAMP or testosterone production. Molecular weights of the products of tryptic cleavage of the hormone were determined using mass spectrometry (MALDI TOF). A comparison of the values obtained with theory shows that the protein is the β-unit of chicken luteinizing hormone.

scholarly journals Characterization of Potential Protein Biomarkers for Major Depressive Disorder Using Matrix-Assisted Laser Desorption Ionization/Time-of-Flight Mass Spectrometry

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4457
Author(s):  
Chieh-Hsin Lin ◽  
Hung Su ◽  
Chung-Chieh Hung ◽  
Hsien-Yuan Lane ◽  
Jentaie Shiea
Keyword(s):  
Mass Spectrometry ◽  
Time Of Flight ◽  
Laser Desorption ◽  
Healthy Controls ◽  
Laser Desorption Ionization ◽  
Major Depressive ◽  
Ionization Time ◽  
Maldi Tof ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Matrix-assisted laser desorption ionization/time-of-flight (MALDI-TOF) mass spectrometry is a sensitive analytical tool for characterizing various biomolecules in biofluids. In this study, MALDI-TOF was used to characterize potential plasma biomarkers for distinguishing patients with major depressive disorder (MDD) from patients with schizophrenia and healthy controls. To avoid interference from albumin—the predominant protein in plasma—the plasma samples were pretreated using acid hydrolysis. The results obtained by MALDI-TOF were also validated by electrospray ionization-quadrupole time-of-flight (ESI-QTOF) mass spectrometry. The analytical results were further treated with principal component analysis (PCA), hierarchical clustering analysis (HCA), and receiver operating characteristic (ROC) curve analysis. The statistical analyses showed that MDD patients could be distinguished from schizophrenia patients and healthy controls by the lack of apolipoprotein C1 (Apo C1), which, in fact, was detected in healthy controls and schizophrenia patients. This protein is suggested to be a potential plasma biomarker for distinguishing MDD patients from healthy controls and schizophrenia patients. Since sample preparation for MALDI-TOF is very simple, high-throughput plasma apolipoprotein analysis for clinical purposes is feasible.

scholarly journals Comparison of Three Approaches to Assess the Flavour Characteristics of Scotch Whisky Spirit

2021 ◽  
Vol 11 (4) ◽  
pp. 1410
Author(s):  
Martina Daute ◽  
Frances Jack ◽  
Irene Baxter ◽  
Barry Harrison ◽  
John Grigor ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Descriptive Analysis ◽  
Analytical Techniques ◽  
Gas Chromatography Mass Spectrometry ◽  
Multiple Factor Analysis ◽  
Quantitative Descriptive Analysis ◽  
Yeast Strains ◽  
Quantitative Descriptive ◽  
Scotch Whisky ◽  
Multiple Factor

This study compared the use of three sensory and analytical techniques: Quantitative Descriptive Analysis (QDA), Napping, and Gas Chromatography-Mass Spectrometry (GC-MS) for the assessment of flavour in nine unmatured whisky spirits produced using different yeasts. Hierarchical Multiple Factor Analysis (HMFA) showed a similar pattern of sample discrimination (RV scores: 0.895–0.927) across the techniques: spirits were mostly separated by their Alcohol by Volume (ABV). Low ABV spirits tended to have heavier flavour characteristics (feinty, cereal, sour, oily, sulphury) than high ABV spirits, which were lighter in character (fruity, sweet, floral, solventy, soapy). QDA differentiated best between low ABV spirits and GC-MS between high ABV spirits, with Napping having the lowest resolution. QDA was time-consuming but provided quantitative flavour profiles of each spirit that could be readily compared. Napping, although quicker, gave an overview of the flavour differences of the spirits, while GC-MS provided semi-quantitative ratios of 96 flavour compounds for differentiating between spirits. Ester, arenes and certain alcohols were found in higher concentrations in high ABV spirits and other alcohols and aldehydes in low ABV spirits. The most comprehensive insights on spirit flavour differences produced by different yeast strains are obtained through the application of a combination of approaches.

Sign in / Sign up

Export Citation Format

Share Document