Synthesis and biological properties of nitrobenzoxadiazole derivatives as potential nitrogen(ii) oxide donors: SOX induction, toxicity, genotoxicity, and DNA protective activity in experiments using Escherichia coli-based lux biosensors

2015 ◽  
Vol 64 (6) ◽  
pp. 1369-1377 ◽  
Author(s):  
V. A. Chistyakov ◽  
Yu. P. Semenyuk ◽  
P. G. Morozov ◽  
E. V. Prazdnova ◽  
V. K. Chmykhalo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Biological Properties ◽  
Protective Activity ◽  
Lux Biosensors

scholarly journals ANTIMICROBIAL POTENTIALS OF SOIL FUNGI METABOLITES, MOLECULAR DYNAMICS AND THEIR 3D PROTEIN STRUCTURAL PREDICTION USING BIOINFORMATICS TOOL

2020 ◽  
pp. 20-26
Author(s):  
MAJOLAGBE O. N. ◽  
AINA D. A. ◽  
OMOMOWO I. O. ◽  
THOMAS A.
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Protein Structure ◽  
Secondary Metabolites ◽  
Soil Fungi ◽  
3D Structure ◽  
Antimicrobial Activities ◽  
Biological Properties ◽  
3D Protein Structure ◽  
Klebsiella Spp

Objective: To determine the antimicrobial potentials of secondary metabolite of soil fungi and predict their 3D structure and molecular identity. Methods: Pure soil fungi were isolated from soil samples and cultured under submerged fermentation (Smf) for their metabolites using Potato Dextrose Agar and Broth. The secondary metabolites of the isolated fungi were obtained intracellularly after 21 d of incubation in a rotary shaker incubator. The antimicrobial potentials of the metabolites were investigated against four (4) clinical isolates, namely: Staphylococcus aureus, Klebsiella spp, Candida albicans and Escherichia coli. These soil fungi were further characterized to the molecular level and their evolutionary relationships established using bioinformatics tools. Protein structure of each of the fungi isolates was predicted using PHYRE-2. Results: Out of all the soil fungi isolated, the metabolite of Aspergillus aculeatus showed the highest antimicrobial activities against Staphylococcus aureus (23.00±2.34 mm), Escherichia coli (9.00±1.44 mm) and Klebsiella spp (24.00±3.45 mm). The 3D protein structure predicted showed that each of the organisms consists of different amino-acid compositions such as: serine, tyrosine, proline, arginine, glycine, phenylalanine leucine with other notable biological properties. Conclusion: The work revealed that secondary metabolites of the isolated fungi carry an important role in combating infectious agents thereby, providing roadmaps for the biosynthesis of many synthetic and semi-synthetic drugs and bio-products which are environmentally friendly.

scholarly journals Thymus mastichina: Composition and Biological Properties with a Focus on Antimicrobial Activity

2020 ◽  
Vol 13 (12) ◽  
pp. 479
Author(s):  
Márcio Rodrigues ◽  
Ana Clara Lopes ◽  
Filipa Vaz ◽  
Melanie Filipe ◽  
Gilberto Alves ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Essential Oil ◽  
Biological Activities ◽  
Scientific Information ◽  
Biological Properties ◽  
Fusarium Spp ◽  
Candida Spp ◽  
Pharmaceutical Industries

Thymus mastichina has the appearance of a semishrub and can be found in jungles and rocky lands of the Iberian Peninsula. This work aimed to review and gather available scientific information on the composition and biological properties of T. mastichina. The main constituents of T. mastichina essential oil are 1,8-cineole (or eucalyptol) and linalool, while the extracts are characterized by the presence of flavonoids, phenolic acids, and terpenes. The essential oil and extracts of T. mastichina have demonstrated a wide diversity of biological activities. They showed antibacterial activity against several bacteria such as Escherichia coli, Proteus mirabilis, Salmonella subsp., methicillin-resistant and methicillin-sensitive Staphylococcus aureus, Listeria monocytogenes EGD, Bacillus cereus, and Pseudomonas, among others, and antifungal activity against Candida spp. and Fusarium spp. Additionally, it has antioxidant activity, which has been evaluated through different methods. Furthermore, other activities have also been studied, such as anticancer, antiviral, insecticidal, repellent, anti-Alzheimer, and anti-inflammatory activity. In conclusion, considering the biological activities reported for the essential oil and extracts of T. mastichina, its potential as a preservative agent could be explored to be used in the food, cosmetic, or pharmaceutical industries.

Specific Lux Biosensors of Escherichia coli Containing pRecA::lux, pColD::lux, and pDinI::lux Plasmids for Detection of Genotoxic Agents

2020 ◽  
Vol 56 (6) ◽  
pp. 666-673
Author(s):  
S. K. Abilev ◽  
V. Y. Kotova ◽  
S. V. Smirnova ◽  
T. N. Shapiro ◽  
G. B. Zavilgelsky
Keyword(s):  
Escherichia Coli ◽  
Genotoxic Agents ◽  
Lux Biosensors

scholarly journals Heat-Stable Enterotoxin of Enterotoxigenic Escherichia coli as a Vaccine Target

2010 ◽  
Vol 78 (5) ◽  
pp. 1824-1831 ◽  
Author(s):  
Arne Taxt ◽  
Rein Aasland ◽  
Halvor Sommerfelt ◽  
James Nataro ◽  
Pål Puntervoll
Keyword(s):  
Escherichia Coli ◽  
Vaccine Development ◽  
Severe Disease ◽  
Epidemiological Data ◽  
Structural Similarity ◽  
Biological Properties ◽  
Cross Reactivity ◽  
Enterotoxigenic Escherichia Coli ◽  
Natural Form ◽  
Heat Stable

ABSTRACT Enterotoxigenic Escherichia coli (ETEC) is responsible for 280 million to 400 million episodes of diarrhea and about 380,000 deaths annually. Epidemiological data suggest that ETEC strains which secrete heat-stable toxin (ST), alone or in combination with heat-labile toxin (LT), induce the most severe disease among children in developing countries. This makes ST an attractive target for inclusion in an ETEC vaccine. ST is released upon colonization of the small intestine and activates the guanylate cyclase C receptor, causing profuse diarrhea. To generate a successful toxoid, ST must be made immunogenic and nontoxic. Due to its small size, ST is nonimmunogenic in its natural form but becomes immunogenic when coupled to an appropriate large-molecular-weight carrier. This has been successfully achieved with several carriers, using either chemical conjugation or recombinant fusion techniques. Coupling of ST to a carrier may reduce toxicity, but further reduction by mutagenesis is desired to obtain a safe vaccine. More than 30 ST mutants with effects on toxicity have been reported. Some of these mutants, however, have lost the ability to elicit neutralizing immune responses to the native toxin. Due to the small size of ST, separating toxicity from antigenicity is a particular challenge that must be met. Another obstacle to vaccine development is possible cross-reactivity between anti-ST antibodies and the endogenous ligands guanylin and uroguanylin, caused by structural similarity to ST. Here we review the molecular and biological properties of ST and discuss strategies for developing an ETEC vaccine that incorporates immunogenic and nontoxic derivatives of the ST toxin.

scholarly journals Production, Purification and Biological Properties of an Escherichia Coli-derived Recombinant Porcine Alpha Interferon

1990 ◽  
Vol 71 (5) ◽  
pp. 1057-1063 ◽  
Author(s):  
F. Lefevre ◽  
R. L'Haridon ◽  
F. Borras-Cuesta ◽  
C. la Bonnardiere
Keyword(s):  
Escherichia Coli ◽  
Biological Properties ◽  
Alpha Interferon

Recombinant HpaA purified from Escherichia coli has biological properties similar to those of native Helicobacter pylori HpaA

Apmis ◽  
2003 ◽  
Vol 111 (3) ◽  
pp. 389-397 ◽  
Author(s):  
ANNELI M. LUNDSTROM ◽  
INGRID BOLIN ◽  
MONA BYSTROM ◽  
SUSANNE NYSTROM
Keyword(s):  
Escherichia Coli ◽  
Helicobacter Pylori ◽  
Biological Properties

scholarly journals Improving the Methanol Tolerance of an Escherichia coli Methylotroph via Adaptive Laboratory Evolution Enhances Synthetic Methanol Utilization

2021 ◽  
Vol 12 ◽  
Author(s):  
R. Kyle Bennett ◽  
Gwendolyn J. Gregory ◽  
Jacqueline E. Gonzalez ◽  
Jie Ren Gerald Har ◽  
Maciek R. Antoniewicz ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Ribosomal Subunit ◽  
Biological Properties ◽  
Methanol Tolerance ◽  
Adaptive Laboratory Evolution ◽  
Laboratory Evolution ◽  
E Coli ◽  
Translational Accuracy ◽  
Methanol Utilization ◽  
Carbon Compounds

There is great interest in developing synthetic methylotrophs that harbor methane and methanol utilization pathways in heterologous hosts such as Escherichia coli for industrial bioconversion of one-carbon compounds. While there are recent reports that describe the successful engineering of synthetic methylotrophs, additional efforts are required to achieve the robust methylotrophic phenotypes required for industrial realization. Here, we address an important issue of synthetic methylotrophy in E. coli: methanol toxicity. Both methanol, and its oxidation product, formaldehyde, are cytotoxic to cells. Methanol alters the fluidity and biological properties of cellular membranes while formaldehyde reacts readily with proteins and nucleic acids. Thus, efforts to enhance the methanol tolerance of synthetic methylotrophs are important. Here, adaptive laboratory evolution was performed to improve the methanol tolerance of several E. coli strains, both methylotrophic and non-methylotrophic. Serial batch passaging in rich medium containing toxic methanol concentrations yielded clones exhibiting improved methanol tolerance. In several cases, these evolved clones exhibited a > 50% improvement in growth rate and biomass yield in the presence of high methanol concentrations compared to the respective parental strains. Importantly, one evolved clone exhibited a two to threefold improvement in the methanol utilization phenotype, as determined via 13C-labeling, at non-toxic, industrially relevant methanol concentrations compared to the respective parental strain. Whole genome sequencing was performed to identify causative mutations contributing to methanol tolerance. Common mutations were identified in 30S ribosomal subunit proteins, which increased translational accuracy and provided insight into a novel methanol tolerance mechanism. This study addresses an important issue of synthetic methylotrophy in E. coli and provides insight as to how methanol toxicity can be alleviated via enhancing methanol tolerance. Coupled improvement of methanol tolerance and synthetic methanol utilization is an important advancement for the field of synthetic methylotrophy.

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