Ocimum basilicum suspension culture as resource for bioactive triterpenoids: yield enrichment by elicitation and bioreactor cultivation

2019 ◽  
Vol 137 (1) ◽  
pp. 65-75 ◽  
Author(s):  
Pallavi Pandey ◽  
Sailendra Singh ◽  
Suchitra Banerjee
Keyword(s):  
Suspension Culture ◽  
Ocimum Basilicum ◽  
Bioreactor Cultivation

IOP Lowering Effects of Ocimum basilicum Seed Extract in Two Rabbit Models of Ocular Hypertension

2019 ◽  
Vol 4 (1) ◽  
pp. 39
Author(s):  
Renu Agarwal ◽  
SK Gupta ◽  
Sushma Srivastava ◽  
Rohit Saxena
Keyword(s):  
Ocular Hypertension ◽  
Antioxidant Properties ◽  
Ocimum Basilicum ◽  
Seed Extract ◽  
Water Loading ◽  
Antiglaucoma Medication ◽  
Lowering Effect ◽  
Significant Difference ◽  
The Mean ◽  
And Control

Introduction: Ocimum basilicum (OB), a herb known for its antihypertensive, anticholinesterase and antioxidant properties was investigated for possible intraocular pressure (IOP) lowering effects in rabbits with ocular hypertension (OHT). Methods: The IOP lowering effect of a single drop of OB extract (OBE) was evaluated in oculonormotensive rabbits using three concentrations (0.25, 0.5 and 1% w/v). The concentration showing maximum IOP reduction was further evaluated in rabbits with water-loading and steroid-induced OHT. Results: IOP lowering effect of OBE 0.5% in oculonormotensive rabbit eyes was significantly greater compared to OBE 0.25% (p<0.05) but was comparable (p>0.05) to OBE 1%. Therefore, 0.5% concentration was selected for further evaluation. Pretreatment with OBE (0.5%) caused significantly lower increase in IOP after water loading amounting to 23.39% above baseline as compared to 54.00% in control eye, 15 minutes post water loading. At 60 minutes, post water loading, mean IOP rise was 95.12% and 63.58% in control and test eyes, respectively. Significant difference between the mean IOP of two eyes persisted during the 2nd hr. In rabbits with steroid induced OHT, OBE 0.5% produced a mean IOP reduction of 24.73% at the end of first hr and the mean peak IOP reduction of 31.63% was observed at the end of 2 hr. A significant difference between the IOP of test and control eyes persisted from 1 to 6 hr. Conclusions: Ocimum basilicum seed extract showed significant IOP lowering effect in rabbits with water loading and steroid induced OHT, however, its utility as an effective antiglaucoma medication needs further investigations.

Drought stress and osmo-priming effects on physiological and biochemical characteristics of Ocimum basilicum

2016 ◽  
Vol 6 (1) ◽  
pp. 905-913
Author(s):  
Bahram Majd Nassiry ◽  
Neda Mohammadi
Keyword(s):  
Drought Stress ◽  
Total Protein ◽  
Catalase Activity ◽  
Seedling Emergence ◽  
Seed Priming ◽  
Ocimum Basilicum ◽  
Field Development ◽  
Physiological And Biochemical Characteristics ◽  
Germination Characteristics ◽  
And Control

    One of the effects of reducing water content on soil is reduction of growth and development of seedlings and variation of field development. Seed priming technique has been known as a challenge to improve germination and seedling emergence under different environmental stresses. The objectives of this research were to evaluate the effects of osmo-priming on germination characteristics and changes of proline, protein and catalase activity of Ocimum basilicum seeds. Results showed that drought stress reduced the germination characteristics and drought stress in -8 bar was the critical stress.  Priming treatments were include KNO3, PEG and NaCl by 0, -4 and -8 bar concentrations. The seeds were primed with those materials for 8 and 16 hours. The highest germination characteristics were obtained from nitrate potassium in -8 bar for 16 hours priming. Therefore the best seed treatment under drought stress during germination was obtained from the osmo-primed with -8 bar nitrate potassium for 16 hours. The drought stress increased proline and catalase activity but reduced total protein. Priming treatment increases proline, total protein and catalase activity under drought and control conditions. It is concluded that priming results in improvement in germination components of Ocimum basilicum in drought stress conditions and increases the resistance to drought stress with improvement of proline, protein and catalase activity in germination phase.

Immobilization of Rhodococcus wratislaviensis Strain KT112-7 Сells in Order to Increase Efficiency of Biodegradation of Modified Polychlorinated Biphenyls

2019 ◽  
pp. 58-70
Author(s):  
M.G. Pervova ◽  
T.I. Gorbunova ◽  
V.A. Demakov
Keyword(s):  
Activated Carbon ◽  
Carbon Fiber ◽  
Polychlorinated Biphenyls ◽  
Suspension Culture ◽  
Chemically Modified ◽  
Commercial Mixture ◽  
Immobilized Culture ◽  
Rhodococcus Wratislaviensis ◽  
Academy Of Sciences ◽  
Integrated Program

The degradation of a mixture of hydroxy- and methoxy-PCB obtained as a result of a chemical modification of a commercial mixture of PCB «Sovol» by the Rhodococcus wratislaviensis strain KT112-7 in plankton culture or immobilized on carbon carriers has been investigated. It was established that the KT112-7 strain in plankton culture degraded 73.2% of a mixture of modified PCBs for 96 h; the strain immobilized on BAU-A activated carbon and on Carbopon-B-active carbon fiber provided the 59.5% and 95.3% degradation for the same time, respectively (with the starting concentration of PBS of 0.5 g/L). The application of the R. wratislaviensis KT112-7 strain immobilized on the BAU-A carrier decreased the number of the PCB derivatives by 1.5 times at the end of the experiment; the corresponding result for the Carbopon-B-active-immobilized culture was 3 times; the set of PCBs after the treatment with the suspension culture remained unchanged. In practice, the immobilization of R. wratislaviensis КТ112-7 strain on carbon carriers can be used in the development of the techniques for the degradation of chemically modified PCB mixtures. polychlorinated biphenyls, immobilization, destruction, utilization, PCB, Rhodococcus. Funding-This work was supported by the Integrated Program of the Ural Branch of the Russian Academy of Sciences No. 18-3-8-19. The work was performed using the equipment of the Center for collective use «Spectroscopy and analysis of organic compounds».

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