Biotechnological potential of a rhizosphere Pseudomonas aeruginosa strain producing phenazine-1-carboxylic acid and phenazine-1-carboxamide

2016 ◽  
Vol 32 (3) ◽  
Author(s):  
Lian Zhou ◽  
Hai-Xia Jiang ◽  
Shuang Sun ◽  
Dan-Dan Yang ◽  
Kai-Ming Jin ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Carboxylic Acid ◽  
Biotechnological Potential ◽  
Rhizosphere Pseudomonas

scholarly journals Colour Me Blue: The History and the Biotechnological Potential of Pyocyanin

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 927
Author(s):  
Thiago Gonçalves ◽  
Ulrich Vasconcelos
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibiotic Therapy ◽  
Electric Current ◽  
Antimicrobial Therapy ◽  
Blue Colour ◽  
Current Generation ◽  
Biotechnological Potential ◽  
Avant Garde ◽  
New Horizons ◽  
The Subject

Pyocyanin was the first natural phenazine described. The molecule is synthesized by about 95% of the strains of Pseudomonas aeruginosa. From discovery up to now, pyocyanin has been characterised by a very rich and avant-garde history, which includes its use in antimicrobial therapy, even before the discovery of penicillin opened the era of antibiotic therapy, as well as its use in electric current generation. Exhibiting an exuberant blue colour and being easy to obtain, this pigment is the subject of the present review, aiming to narrate its history as well as to unveil its mechanisms and suggest new horizons for applications in different areas of engineering, biology and biotechnology.

Simultaneous production of three phenazine pigments by Pseudomonas aeruginosa Mac 436

1969 ◽  
Vol 15 (5) ◽  
pp. 439-444 ◽  
Author(s):  
P. C. Chang ◽  
A. C. Blackwood
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Carboxylic Acid ◽  
Simultaneous Production ◽  
Culture Liquor ◽  
The Media

Pseudomonas aeruginosa Mac 436 was found to produce simultaneously three phenazine pigments identified as pyocyanine, phenazine-1-carboxylic acid, and oxychlororaphine. Production of these pigments on various media indicated a wide variation in yields depending on the composition of the media, but satisfactory yields of all three pigments were obtained. A scheme was developed for separation and assay of the pigments from the culture liquor. Details of production, isolation, assay, and identification are given.

scholarly journals Elucidation and Identification of an Antifungal Compound from Pseudomonas aeruginosa DA3.1 Isolated from Soil in Vietnam

2020 ◽  
Vol 13 (10) ◽  
Author(s):  
Nguyen Thi Trung ◽  
Nguyen Tien Cuong ◽  
Nguyen Thi Thao ◽  
Dao Thi Mai Anh ◽  
Do Thi Tuyen
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Pseudomonas Aeruginosa ◽  
Antifungal Activity ◽  
Carboxylic Acid ◽  
Magnetic Resonance ◽  
13C Nmr ◽  
Bacterial Strain ◽  
Antifungal Compound ◽  
Fusarium Sp ◽  
High Antifungal Activity

Background: Fusarium sp. and Rhizoctonia sp. fungi have been always threats to short-term crops. In Vietnam, corn and soybean suffer serious losses annually. Therefore, it is necessary to utilize an environmentally friendly antifungal compound that is highly effective against phytopathogenic fungi. Pseudomonas sp. is a popular soil bacterial strain and well known for its high antifungal activity. Objectives: This study was carried out to evaluate and assess the antifungal activity of a local bacterial strain namely DA3.1 that was later identified as Pseudomonas aeruginosa. This would be strong scientific evidence to develop an environmentally friendly biocide from a local microorganism strain for commercial use. Methods: The antifungal compound was purified from ethyl acetate extraction of deproteinized cell culture broth by a silica gel column (CH2Cl2/MeOH (0% - 10% MeOH)). The purity of the isolated compound was determined by HPLC, and its molecular structure was elucidated using spectroscopic experiments including one-dimensional (1D) (1H NMR, 13C NMR, DEPT) and two-dimensional (2D) (HMBC and HSQC) spectra. The activity of the purified compound against Fusarium sp. and Rhizoctonia sp. fungi was measured using the PDA-disk diffusion method, and its growth-promoting ability was evaluated using the seed germination test of corn and soybean. Results: The results showed that the antifungal compound produced by Pseudomonas aeruginosa DA3.1 had a retention factor (Rf) of 0.86 on thin layer chromatography (TLC). Based on the evidence of spectral data including proton nuclear magnetic resonance (1H NMR), carbon nuclear magnetic resonance (13C NMR), distortionless enhancement by polarization transfer (DEPT), heteronuclear multiple bond correlation (HMBC), and heteronuclear single quantum coherence (HSQC), the chemical structure was elucidated as phenazine-1-carboxylic. The purified compound showed inhibitory activity against F. oxysporum and R. solani and exhibited the ability of the germination of corn and soybean seeds. The results revealed the benefit of native P. aeruginosa DA3.1 and phenazine-1-carboxylic acid for use as a biocontrol agent, as well as a plant growth promoter. Conclusions: The antifungal compound isolated from local Pseudomonas DA3.1 was identified as phenazine-1-carboxylic acid that posed high antifungal activity and was a plant germination booster.

Influence of Mineral Salts on the Production of Antibiotics Phenazine Series from Pseudomonas Aeruginosa

2016 ◽  
Vol 685 ◽  
pp. 794-797
Author(s):  
Yekaterina S. Palchevskaya
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Carboxylic Acid ◽  
Disease Management ◽  
Secondary Metabolites ◽  
Broad Spectrum ◽  
Plant Disease ◽  
Mineral Salts ◽  
Heterocyclic Nitrogen ◽  
Plant Disease Management ◽  
Nitrogen Containing Compounds

Phenazines represent a group of heterocyclic nitrogen-containing compounds showing a broad spectrum of antibiotic properties. Phenazines are studied extensively for their further application in plant disease management. Pseudomonas aeruginosa produce phenazine compounds as the secondary metabolites. In this paper a complex of phenazine series antibiotics from the culture of Pseudomonas aeruginosa was isolated and studied. It was established that the complex represented by phenazine-1-carboxylic acid and 2-hydroxyphenazine. The influence of various mineral salts to produce phenazine was investigated. Inhibitors and cofactors of the biosynthesis of antibiotics phenazine series were determined.

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