Simultaneous production of three phenazine pigments by Pseudomonas aeruginosa Mac 436

1969 ◽  
Vol 15 (5) ◽  
pp. 439-444 ◽  
Author(s):  
P. C. Chang ◽  
A. C. Blackwood
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Carboxylic Acid ◽  
Simultaneous Production ◽  
Culture Liquor ◽  
The Media

Pseudomonas aeruginosa Mac 436 was found to produce simultaneously three phenazine pigments identified as pyocyanine, phenazine-1-carboxylic acid, and oxychlororaphine. Production of these pigments on various media indicated a wide variation in yields depending on the composition of the media, but satisfactory yields of all three pigments were obtained. A scheme was developed for separation and assay of the pigments from the culture liquor. Details of production, isolation, assay, and identification are given.

scholarly journals EXPERIMENTAL DESIGN FOR SIMULTANEOUS PRODUCTION OF PHB, MESOPHILIC PROTEASES AND LIPASES

2011 ◽  
Vol 12 (2) ◽  
pp. 155-184 ◽  
Author(s):  
Amro Abd al fattah Amara
Keyword(s):  
Statistical Analysis ◽  
Experimental Design ◽  
Bacillus Pumilus ◽  
Bacillus Species ◽  
Bacillus Sp ◽  
Box Behnken Design ◽  
Simultaneous Production ◽  
The Media ◽  
Excel Solver

ABSTRACT: Bacillus species are able to produce PHB, Proteases and Lipases. Bacillus subtilius, Bacillus pumilus, Bacillus therigienesis and Bacillus sp. were used. Plackett-Burman, Box-Behnken design and the Excel solver were used to optimize the production. The statistical analysis of the results proved insignificant relationship between the media compositions and the responses. The results clearly proved a competition between the production of PHB, Proteases and Lipases. Meanwhile systematic experimental design succeeded to minimize this competition. The maximum gained PHB in this study were 16.48 g/l/48 hr. In case of Proteases and Lipases were 534, and 22.56 Units/ml/48 h respectively. The strategies used in this study are recommended for simultaneous production of PHB and proteases. For some extend lipases produced too.

scholarly journals EVALUATION OF DIFFERENT CULTURE MEDIA FOR ENHANCED PRODUCTION OF PSEUDOMONAS AERUGINOSA (MTCC NO 2453) BIOMASS AND ITS PROTEINS

2016 ◽  
Vol 8 (11) ◽  
pp. 120 ◽  
Author(s):  
P. Neeraja ◽  
T. Parthasarathy ◽  
M. Sudhakar
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Protein Production ◽  
Culture Media ◽  
Batch Cultivation ◽  
Luria Bertani ◽  
Shake Flask Culture ◽  
Cell Biomass ◽  
Enhanced Production ◽  
Anti Cancer ◽  
The Media

Objective: Microorganisms, especially bacteria and its proteins have proven to be potential anti-cancer agents as they selectively attack the tumor cells or tumor micro-environments. The extract of Pseudomonas aeruginosa found to contain proteins that have shown promising anticancer activity. In this work, it was attempted to increase the biomass and trigger the total protein fraction of Pseudomonas aeruginosa (MTCC 2453).Methods: The organism was cultivated in three different such as Luria-Bertani (LB) broth, minimal medium9 (M9), super broth medium (SB) and asparagine-proline (AP) broth. Asparagine proline broth was selected as it has shown high cell growth rate. The media was further optimized by the addition of NaHCO3 and copper sulphate to trigger the protein production. Optimized Aspergine proline broth has achieved highest cell biomass. After the shake flask culture, the overnight grown culture in optimized AP medium was further grown in a 5 L bioreactor by fed-batch cultivation to achieve higher cell densities.Results: The highest protein production was achieved at 40 ° C. Highest biomass and protein content was observed at pH 8 while lowest biomass was produced at pH 2. A gradual increase in biomass content observed from 12 h towards to 48 h.Conclusion: High biomass and proteins content and of Pseudomonas aeruginosa (MTCC 2453) can be produced in optimized asparagine-proline broth. Further the extract is purified to produce novel anti-cancer proteins.

scholarly journals Elucidation and Identification of an Antifungal Compound from Pseudomonas aeruginosa DA3.1 Isolated from Soil in Vietnam

2020 ◽  
Vol 13 (10) ◽  
Author(s):  
Nguyen Thi Trung ◽  
Nguyen Tien Cuong ◽  
Nguyen Thi Thao ◽  
Dao Thi Mai Anh ◽  
Do Thi Tuyen
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Pseudomonas Aeruginosa ◽  
Antifungal Activity ◽  
Carboxylic Acid ◽  
Magnetic Resonance ◽  
13C Nmr ◽  
Bacterial Strain ◽  
Antifungal Compound ◽  
Fusarium Sp ◽  
High Antifungal Activity

Background: Fusarium sp. and Rhizoctonia sp. fungi have been always threats to short-term crops. In Vietnam, corn and soybean suffer serious losses annually. Therefore, it is necessary to utilize an environmentally friendly antifungal compound that is highly effective against phytopathogenic fungi. Pseudomonas sp. is a popular soil bacterial strain and well known for its high antifungal activity. Objectives: This study was carried out to evaluate and assess the antifungal activity of a local bacterial strain namely DA3.1 that was later identified as Pseudomonas aeruginosa. This would be strong scientific evidence to develop an environmentally friendly biocide from a local microorganism strain for commercial use. Methods: The antifungal compound was purified from ethyl acetate extraction of deproteinized cell culture broth by a silica gel column (CH2Cl2/MeOH (0% - 10% MeOH)). The purity of the isolated compound was determined by HPLC, and its molecular structure was elucidated using spectroscopic experiments including one-dimensional (1D) (1H NMR, 13C NMR, DEPT) and two-dimensional (2D) (HMBC and HSQC) spectra. The activity of the purified compound against Fusarium sp. and Rhizoctonia sp. fungi was measured using the PDA-disk diffusion method, and its growth-promoting ability was evaluated using the seed germination test of corn and soybean. Results: The results showed that the antifungal compound produced by Pseudomonas aeruginosa DA3.1 had a retention factor (Rf) of 0.86 on thin layer chromatography (TLC). Based on the evidence of spectral data including proton nuclear magnetic resonance (1H NMR), carbon nuclear magnetic resonance (13C NMR), distortionless enhancement by polarization transfer (DEPT), heteronuclear multiple bond correlation (HMBC), and heteronuclear single quantum coherence (HSQC), the chemical structure was elucidated as phenazine-1-carboxylic. The purified compound showed inhibitory activity against F. oxysporum and R. solani and exhibited the ability of the germination of corn and soybean seeds. The results revealed the benefit of native P. aeruginosa DA3.1 and phenazine-1-carboxylic acid for use as a biocontrol agent, as well as a plant growth promoter. Conclusions: The antifungal compound isolated from local Pseudomonas DA3.1 was identified as phenazine-1-carboxylic acid that posed high antifungal activity and was a plant germination booster.

Influence of Mineral Salts on the Production of Antibiotics Phenazine Series from Pseudomonas Aeruginosa

2016 ◽  
Vol 685 ◽  
pp. 794-797
Author(s):  
Yekaterina S. Palchevskaya
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Carboxylic Acid ◽  
Disease Management ◽  
Secondary Metabolites ◽  
Broad Spectrum ◽  
Plant Disease ◽  
Mineral Salts ◽  
Heterocyclic Nitrogen ◽  
Plant Disease Management ◽  
Nitrogen Containing Compounds

Phenazines represent a group of heterocyclic nitrogen-containing compounds showing a broad spectrum of antibiotic properties. Phenazines are studied extensively for their further application in plant disease management. Pseudomonas aeruginosa produce phenazine compounds as the secondary metabolites. In this paper a complex of phenazine series antibiotics from the culture of Pseudomonas aeruginosa was isolated and studied. It was established that the complex represented by phenazine-1-carboxylic acid and 2-hydroxyphenazine. The influence of various mineral salts to produce phenazine was investigated. Inhibitors and cofactors of the biosynthesis of antibiotics phenazine series were determined.

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