Isolation and identification of temperature tolerant phosphate solubilizing bacteria as a potential microbial fertilizer

2019 ◽  
Vol 35 (8) ◽  
Author(s):  
Mohammad Reza Sarikhani ◽  
Bahman Khoshru ◽  
Ralf Greiner
Keyword(s):  
Phosphate Solubilizing Bacteria ◽  
Isolation And Identification ◽  
Phosphate Solubilizing

scholarly journals Isolation and identification of phosphate solubilizing bacteria and evaluate its effect on of Mung bean (Vigna radita [L.] R.Wilczek) growth

2019 ◽  
Vol 60 (5) ◽  
pp. 985-995
Author(s):  
Yusur Ramzi ◽  
Hutaf A. A. Alsalim
Keyword(s):  
Plant Growth ◽  
Bacillus Cereus ◽  
Mung Bean ◽  
Phosphate Solubilization ◽  
Growth Promotion ◽  
Hydrolytic Enzymes ◽  
Phosphate Solubilizing Bacteria ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Phosphate Solubilizing

Sixteen soil samples were collected from wheat, barley and yellow corn rhizosphere in Abu-Ghraib, Aqraqof, Latifieh,Tarmiah, Jadriya and  of Agriculture in Baghdad university/ Baghdad city. The results found nine phosphate solubilizing bacteria (PSB) isolates (Y1, Y2, Y3, Y4, Y5, Y6, Y7, Y8, Y9), formed clear zones on National Botanical Research Institute's (NBRIP) agar. The solubility index (SI) of PSB isolates ranged from 2.00 to 3.66. Y4 have the highest SI (3.66) followed by Y3 and Y6 (3.33). Phosphate solubilization abilities varying from (20.10-39.00 μg.ml-1), Y4 was the highest (39.00 μg.ml-1) followed by Y3 (37.00μg.ml-1). The results of hydrolytic enzymes production showed that almost all nine isolates are able to produce protease and pectinase, while Y1 and Y2 showed negative results in cellulase production. Maximum ability for hydrogen cyanide (HCN) and indole acetic acid (IAA) production were showed byY3 and Y4 isolates. The isolate Y4 was found to be the most efficient isolate, so it was selected identified as Bacillus cereus using biochemical tests confirmed by VITEC 2 compact system. The results of High performance liquid chromatography (HPLC) revealed that Bacillus cereus produce oxalic acid (2.996), citric acid (9.117) and malic acid (3.734). Bacillus cereus (Y4) enhanced the growth of mung bean plants. A significant increase in branches number (12.33), plant length (83.0cm), fresh weight (27.25 g) and dry weight (1.427g) were obtained compared with control treatments. The main objective of this study is to isolate PSB and evaluate their roles in plant growth promotion. The results showed the high phosphate solubilization efficiency of PSB isolates and the identified isolates was found to be good enough for plant growth promoting.

scholarly journals Isolasi Rhizobakteri dan Pengaruh Aplikasinya dengan Pupuk N-P terhadap Mutu Benih dan Pertumbuhan Bibit Tetua Betina Jagung

2018 ◽  
Vol 45 (3) ◽  
pp. 255
Author(s):  
Pitri Ratna Asih ◽  
Memen Surahman ◽  
Dan Giyanto
Keyword(s):  
Field Experiments ◽  
Seed Quality ◽  
Female Parent ◽  
Dry Weight ◽  
Hybrid Seed ◽  
Phosphate Solubilizing Bacteria ◽  
Isolation And Identification ◽  
Seed Field ◽  
P Fertilizer ◽  
Phosphate Solubilizing

Increased productivity of maize can be done with the use of high quality seeds from improved varieties such as hybrid seed. The objectives of this study were Increasing productivity of maize female parent is important in order to reduce the price of hybrid seed. The objectives of this study were to determine the nitrogen fixing bacteria compatible with phosphate solubilizing bacteria (PSB), and using those bacteria to increase physiological seed quality and seedling growth of maize female parent. The research consisted of laboratory and field experiments. Laboratory experiment for the isolation and identification of rhizobacteria resulted in 25 Azotobacter and 29 Actinomycetes non-pathogenic isolates capable of fixing nitrogen and PSB selected for compatibility tests were AB3, B28, P12, P14, P24, and P31. The compatibility test showed 25 pairs of BPF with Azotobacter and 16 pairs of BPF with Actinomycetes were mutually compatible. The BPF pair with Azotobacter or Actinomycetes P24-AzL7, P24-AzL9, B28-AcCKB4, P24-AcCKB9, P24-AcCKB20, and P24-AcCKW5 were able to increase the vigor index of hybrid maize female parent seed. Field experiment was arranged in a split plot design with three replications. The main plot was dosage of N-P fertilizer (0%, 25%, 50%, 75%, and 100% of recommendation dosage), and the subplot was 12 rhizobacteria treatments selected from 25 compatible pairs of BPF with Azotobacter and 16 pairs of BPF with Actinomycetes and 1 control. The application of compatible pairs of bacteria had a significant effect on plant height, the number of leaves at 3 and 4 weeks after planting and plant dry weight. However, the best treatment i.e. B28-AcCKB4 was not significantly different with the nutrient broth treatment (as control).<br /><br /><br />

scholarly journals Isolation and Identification of Hydrocarbon Degradation Bacteria and Phosphate Solubilizing Bacteria in Oil Contaminated Soil in Bojonegoro, East Java, Indonesia

2019 ◽  
Vol 4 (1) ◽  
pp. 134
Author(s):  
Yuni Sri Rahayu
Keyword(s):  
Organic Compounds ◽  
Contaminated Soil ◽  
Soil Samples ◽  
Identification System ◽  
Phosphate Solubilizing Bacteria ◽  
Bacterial Cells ◽  
Isolation And Identification ◽  
Degrading Bacteria ◽  
Phosphate Solubilizing ◽  
Physiological Tests

Petroleum is a mixture of hydrocarbon complexes with organic compounds from sulfur, oxygen, nitrogen and metal-containing compounds. These organic compounds can be used as substrate for bacterial growth. This study aimed to isolate and identify hydrocarbon degrading bacteria and phosphate solubilizing bacteria in oil-contaminated soil in Bojonegoro. This study used an exploration method to find each of the two types of hydrocarbon degrading bacteria and phosphate solubilizing bacteria from soil samples in Bojonegoro that contaminated by oil. Identification of isolates bacterial included macroscopic observations of bacteria, gram staining on bacterial cells and physiological tests. Macroscopic observations include the form of colonies, colony diameter, colony color, colony edge, and elevation. The physiological test using Microbact Identification System to determine the physiological characteristics of bacteria so that genera and types of bacteria can be known. The identification of organisms was based on changes in pH and use of the substrate. The results of data analysis were obtained from five types of bacteria from soil samples that contaminated by oil which were successfully isolated. After identification of species was done, four species of bacteria were obtained, namely Pseudomonas pseudomallei, Pseudomonas fluorescens-25, Flavobacterium odoratum, and Enterococcus sp.

scholarly journals Isolation and Identification of Elite Phosphate Solubilizing Bacteria from Soil under Paddy Cultivation

2014 ◽  
Vol 16 ◽  
pp. 62-68 ◽  
Author(s):  
A. Gandhi ◽  
G. Muralidharan ◽  
E. Sudhakar
Keyword(s):  
Bacterial Species ◽  
Essential Element ◽  
Broth Culture ◽  
Bacillus Species ◽  
Available Phosphorus ◽  
Growth Media ◽  
Screening Methods ◽  
Phosphate Solubilizing Bacteria ◽  
Isolation And Identification ◽  
Phosphate Solubilizing

A considerable number of bacterial species are able to exert a beneficial effect upon plant growth. Mostly they are associated with the plant rhizosphere, so they are called as rhizobacteria. Phosphorus is an essential element for plant development and growth making up about 0.2 % of plant dry weight. Several scientists have reported the ability of different bacterial species to solubilize insoluble inorganic phosphate compounds, such as tricalcium phosphate, dicalcium phosphate, hydroxyapatite, and rock phosphate. Detection and estimation of the phosphate solublization ability of microorganisms have been possible using plate screening methods. Phosphate solubilizers produce clearing zones around the microbial colonies in growth media. In the present investigation a total number of fifteen phosphate solubilizing bacterial colonies isolated from different paddy soils in Cuddalore district of Tamilnadu, India. The isolated PSB were identified and characterized for effective use in the field. All the PSB isolates were identified as Bacillus species and designated as P with serial number from 1 to 15. Among the fifteen isolates, the PSB isolate P6 showed highest amount of phosphate solubilization. The quantity of available phosphorus estimated in the P6 grown Sperber broth culture medium on 7th day was maximum of 321.7 μg/ml which was the highest value compared to other PSB isolates.

scholarly journals Isolation and Identification of Uranium Tolerant Phosphate-Solubilizing Bacillus spp. and Their Synergistic Strategies to U(VI) Immobilization

2021 ◽  
Vol 12 ◽  
Author(s):  
Juan Zhong ◽  
Xuewu Hu ◽  
Xingyu Liu ◽  
Xinglan Cui ◽  
Ying Lv ◽  
...  
Keyword(s):  
Extracellular Polymeric Substances ◽  
Rrna Genes ◽  
Phosphate Solubilizing Bacteria ◽  
Ftir Analysis ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Interesting Phenomenon ◽  
Bacterial Surface ◽  
Bacillus Spp ◽  
Phosphate Solubilizing

The remediation of uranium (U) through phosphate-solubilizing bacteria (PSB) is an emerging technique as well as an interesting phenomenon for transforming mobile U into stable minerals in the environment. While studies are well needed for in-depth understanding of the mechanism of U(VI) immobilization by PSB. In this study, two PSB were isolated from a U-tailing repository site. These bacterial strains (ZJ-1 and ZJ-3) were identified as Bacillus spp. by the sequence analysis of 16S ribosomal RNA (rRNA) genes. Incubation of PSB in liquid medium showed that the isolate ZJ-3 could solubilize more than 230 mg L–1 P from glycerol-3-phosphate and simultaneously removed over 70% of 50 mg L–1 U(VI) within 1 h. During this process, the rapid appearance of yellow precipitates was observed. The microscopic and spectroscopic analysis demonstrated that the precipitates were associated with U-phosphate compound in the form of saleeite-like substances. Besides, scanning electron microscopy coupled with energy-dispersive X-ray (SEM-EDS) and Fourier transform infrared spectroscopy (FTIR) analysis of the precipitates confirmed that the extracellular polymeric substances (EPS) might also play a key role in U sequestration. Furthermore, SEM and FTIR analysis revealed that part of U(VI) was adsorbed on the bacterial surface through cellular phosphate, hydroxy, carboxyl, and amide groups. This study provides new insights into the synergistic strategies enhancing U immobilization rates by Bacillus spp. that uses glycerol-3-phosphate as the phosphorus source, the process of which contributes to harmful pollutant biodegradation.

scholarly journals Isolation and Identification of Phosphate Solubilizing and Nitrogen-Fixing Bacteria from Lake Ol’Bolossat Sediments, Kenya

2020 ◽  
Vol 14 (10) ◽  
pp. 37
Author(s):  
Eliud Nalianya Wafula ◽  
Sylvia Injete Murunga
Keyword(s):  
Agar Medium ◽  
Vital Role ◽  
Rrna Gene ◽  
Phosphate Solubilizing Bacteria ◽  
Nitrogen Fixing ◽  
Genus Bacillus ◽  
Isolation And Identification ◽  
Nitrogen Fixing Bacteria ◽  
Microbial Counts ◽  
Phosphate Solubilizing

Phosphate solubilizing and nitrogen-fixing bacteria have a vital role in improving soil fertility and reverting adversely affected soil properties. These bacteria could contribute towards sustainable agriculture with a focus on reducing excessive use of commercial fertilizers. This study aimed at investigating autochthonous populations of phosphate solubilizing and nitrogen-fixing bacteria from Lake Ol&rsquo;Bolossat sediments. The total microbial counts ranged between 4.8 x 103 to 8.5 x 105 cfu/ml. A total of 50 bacteria were isolated, 34 were obtained from Pikovskaya&rsquo;s agar medium while 16 were obtained from Norris Glucose Nitrogen free medium. Based on morphological and 16S rRNA gene analyses, the isolates were clustered under the genera Bacillus, Arthrobacter, Pseudomonas, Paenibacillus, Fictibacillus and Acinetobacter. Among potentially novel strains, four strains NFDA2, PKGBC1 (MT799539), PKGB5 and SCEC2 (MT799543) belonged to genus Bacillus, three strains NFGA1 (MT799529), NFGA4 and SCDB3 belonged to the genus Pseudomonas, two strains NFEB6 (MT799528) and NFDC5 belonged to the genus Paenibacillus, one strain PKHC3 (MT7995441) belonged to the genus Arthrobacter while one strain NFDC4, belonged to the genus Acinetobacter. Generally, the phosphate solubilizing bacteria were the most diverse and genera Bacillus, Fictibacillus and Pseudomonas were the most dominant, however, nitrogen-fixing bacteria were dominated by genera Arthrobacter and Pseudomonas.

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