Gene-ecological zonation and population genetic structure of Tectona grandis L.f. in India revealed by genome-wide SSR markers

Following its recent invasion of North America, the soybean aphid (Aphis glycines Matsumura) has become the number one insect pest of soybean (Glycine max L. Merr.) in the north central states of the USA. A few studies have been conducted on the population genetic structure and genetic diversity of the soybean aphid and the source of its invasion in North America. Molecular markers, such as simple sequence repeats (SSRs) are very useful in the evaluation of population structure and genetic diversity. We used 18 SSR markers to assess the genetic diversity of soybean aphid collections from the USA, South Korea, and Japan. The aphids were collected from two sites in the USA (Indiana and South Dakota), two sites in South Korea (Yeonggwang district and Cheonan city), and one site in Japan (Utsunomiya). The SSR markers were highly effective in differentiating among aphid collections from different countries. The level of differentiation within each population and among populations from the same country was limited, even in the case of the USA where the two collection sites were more than 1200 km apart.

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Population genetic structure based on SSR markers in alfalfa (Medicago sativa L.) from various regions contiguous to the centres of origin of the species

Journal of Genetics ◽

10.1007/s12041-007-0008-9 ◽

2007 ◽

Vol 86 (1) ◽

pp. 59-63 ◽

Cited By ~ 15

Author(s):

M. Falahati-Anbaran ◽

A. A. Habashi ◽

M. Esfahany ◽

S. A. Mohammadi ◽

B. Ghareyazie

Keyword(s):

Genetic Structure ◽

Medicago Sativa ◽

Ssr Markers ◽

Population Genetic Structure ◽

Population Genetic ◽

Medicago Sativa L

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Genome-wide developed microsatellites reveal a weak population differentiation in the hoverflyEupeodes corollae(Diptera: Syrphidae) across China

10.1101/606657 ◽

2019 ◽

Author(s):

Meng-Jia Liu ◽

Xiao-qiang Wang ◽

Ling Ma ◽

Li-Jun Cao ◽

Hong-Ling Liu ◽

...

Keyword(s):

Genetic Structure ◽

Population Genetic Structure ◽

Population Genetic ◽

Northern China ◽

High Rate ◽

Genomic Sequences ◽

Genome Wide ◽

Geographical Populations ◽

Plant Pollinator ◽

Strict Selection

AbstractThe hoverfly,Eupeodes corollae,is a worldwide natural enemy of aphids and a plant pollinator. To provide insights into the biology of this species, we examined its population genetic structure by obtaining 1.15-GB random genomic sequences using next-generation sequencing and developing genome-wide microsatellite markers. A total of 79,138 microsatellite loci were initially isolated from the genomic sequences; after strict selection and further testing of 40 primer pairs in eight individuals, 24 polymorphic microsatellites with high amplification rates were developed. These microsatellites were used to examine the population genetic structure of 96 individuals from four field populations collected across southern to northern China. The number of alleles per locus ranged from 5 to 13 with an average of 8.75; the observed and expected heterozygosity varied from 0.235 to 0.768 and from 0.333 to 0.785, respectively. Population genetic structure analysis showed weak genetic differentiation among the four geographical populations ofE. corollae, suggesting a high rate of gene flow reflecting likely widespread migration ofE. corollaein China.

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Genome-wide analysis clarifies the population genetic structure of wild gilthead sea bream (Sparus aurata)

PLoS ONE ◽

10.1371/journal.pone.0236230 ◽

2021 ◽

Vol 16 (1) ◽

pp. e0236230

Author(s):

Francesco Maroso ◽

Konstantinos Gkagkavouzis ◽

Sabina De Innocentiis ◽

Jasmien Hillen ◽

Fernanda do Prado ◽

...

Keyword(s):

Genetic Structure ◽

Population Genetic Structure ◽

Population Genetic ◽

Gilthead Sea Bream ◽

Sea Bream ◽

North East ◽

Genome Wide ◽

Distribution Ranges ◽

In The Wild ◽

The Mediterranean

Gilthead sea bream is an important target for both recreational and commercial fishing in Europe, where it is also one of the most important cultured fish. Its distribution ranges from the Mediterranean to the African and European coasts of the North-East Atlantic. Until now, the population genetic structure of this species in the wild has largely been studied using microsatellite DNA markers, with minimal genetic differentiation being detected. In this geographically widespread study, 958 wild gilthead sea bream from 23 locations within the Mediterranean Sea and Atlantic Ocean were genotyped at 1159 genome-wide SNP markers by RAD sequencing. Outlier analyses identified 18 loci potentially under selection. Neutral marker analyses identified weak subdivision into three genetic clusters: Atlantic, West, and East Mediterranean. The latter group could be further subdivided into an Ionian/Adriatic and an Aegean group using the outlier markers alone. Seascape analysis suggested that this differentiation was mainly due to difference in salinity, this being also supported by preliminary genomic functional analysis. These results are of fundamental importance for the development of proper management of this species in the wild and are a first step toward the study of the potential genetic impact of the sea bream aquaculture industry.

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