Metabolomic study of polyamines in rat urine following intraperitoneal injection of γ-hydroxybutyric acid

Metabolomics ◽  
2019 ◽  
Vol 15 (4) ◽  
Author(s):  
Hyeon-Seong Lee ◽  
Chan Seo ◽  
Young-A Kim ◽  
Meejung Park ◽  
Boyeon Choi ◽  
...  
Keyword(s):  
Intraperitoneal Injection ◽  
Hydroxybutyric Acid ◽  
Rat Urine ◽  
Metabolomic Study

scholarly journals Metabolomic Study of Polyamines in Rat Urine Following Intraperitoneal Injection of γ-Hydroxybutyric Acid

2018 ◽  
Author(s):  
Hyeon-Seong Lee ◽  
Chan Seo ◽  
Young-A Kim ◽  
Meejung Park ◽  
Boyeon Choi ◽  
...  
Keyword(s):  
Intraperitoneal Injection ◽  
Gas Chromatography Mass Spectrometry ◽  
Control Group ◽  
Hydroxybutyric Acid ◽  
Multivariate Statistical ◽  
Rat Urine ◽  
Metabolic Pattern ◽  
Star Pattern ◽  
Multiple Administration ◽  
Metabolomic Study

1) Background: Recently, illegal abuse of γ-hydroxybutyric acid (GHB) has increased in drug-facilitated crimes, but determination of GHB exposure and intoxication is difficult due to rapid metabolism of GHB. Its biochemical mechanism has not been completely investigated. And metabolomic study by polyamine profile and pattern analyses was not performed in rat urinefollowing intraperitoneal injection with GHB. 2) Methods: Polyamine profiling analysis by gas chromatography-mass spectrometry combined with star pattern recognition analysis was performed in this study. Multivariate statistical analysis was used to evaluate discrimination between control and GHB administration groups. 3) Results: Six polyamines were determined in control, single and multiple GHB administration groups. Star pattern showed distorted hexagonal shapes with characteristic and readily distinguishable patterns for each group. N1-Acetylspermine (p < 0.001), putrescine (p <0.006), N1-acetylspermidine (p <0.009), and spermine (p < 0.027) were significantly increased in single administration group but were significantly lower in the multiple administration group than in the control group. N1-Acetylspermine was the main polyamine for discrimination between control, single and multiple administration groups. Spermine showed similar levels in single and multiple administration groups. 4) Conclusions: The polyamine metabolic pattern was monitored in GHB administration groups. N1-Acetylspermine and spermine were evaluated as potential biomarkers of GHB exposure and addiction.

Monitoring of altered amino acid metabolic pattern in rat urine following intraperitoneal injection with γ-hydroxybutyric acid

Metabolomics ◽  
2018 ◽  
Vol 14 (9) ◽  
Author(s):  
Chan Seo ◽  
Myungjin Na ◽  
Jiyeun Jang ◽  
Meejung Park ◽  
Boyeon Choi ◽  
...  
Keyword(s):  
Amino Acid ◽  
Intraperitoneal Injection ◽  
Hydroxybutyric Acid ◽  
Rat Urine ◽  
Metabolic Pattern

scholarly journals The metabolism of labelled hexadecyl sulphate salts in the rat, dog and human

1975 ◽  
Vol 148 (2) ◽  
pp. 219-225 ◽  
Author(s):  
I Merits
Keyword(s):  
The Other ◽  
Hydroxybutyric Acid ◽  
Metabolic Fate ◽  
Main Metabolite ◽  
Rat Urine ◽  
Glycollic Acid ◽  
Dilution Experiments ◽  
Solvent Systems ◽  
A Minor ◽  
Sulphate Salts

The metabolic fate of [1-14-C]hexadecylsulphate and hexadecyl[35-S]sulphate, administered intravenously as the sodium and trimethylammonium salt to dogs and orally as the erythromycin salt to dogs, rats and humans, was studied. Studies with rats indicated that the compounds were well absorbed and rapidly excreted in the urine. However, after oral administration of the 14-C-and 35-S-labelled hexadecyl sulphate erythromycin salt to dogs, considerable amounts of radioactivity were excreted in the faeces as unmetabolized hexadecyl sulphate. Studies with two humans showed that orally administered erythromycin salt of [1-14C]hexadecyl sulphate was well absorbed in one person but poorly absorbed in the other. Radioactive metabolites in urine were separated by t.l.c. in two solvent systems. The main metabolite of hexadecyl sulphate in the dog, rat and human was identified as the sulphate ester of 4-hydroxybutyric acid. In addition, psi-[14-C]butyrolactone as a minor metabolic product of [1-14-C]hexadecyl sulphate was also isolated from the urine of rat, dog and man. However, there was still another metabolite in dog urine, which comprised about 20% of the total urinary radioactivity and carried both 14-C and 35-S labels. This metabolite was absent from rat urine. The metabolite in dog urine was isolated and subsequently identified by t.l.c. and g.l.c. and by isotope-dilution experiments as the sulphate ester of glycollic acid. Small amounts (about 5% of the total recovered radioactivity in excreta) of labelled glycollic acid sulphate were also found in human urine after ingestion of erythromycin [1-14-C]hexadecyl sulphate.

Clinical Characterization of High-Dose Gamma-Hydroxybutyric Acid (GHB) Intoxication

2006 ◽  
Author(s):  
Matthew W. Johnson ◽  
Lawrence P. Carter ◽  
Annie Umbricht ◽  
Roland R. Griffiths
Keyword(s):  
High Dose ◽  
Hydroxybutyric Acid

DIE INITIALE VERTEILUNG VON RADIO-C IN DEN ZELLFRAKTIONEN EINIGER RATTENORGANE NACH INJEKTION VON TESTOSTERON-4-14C

1964 ◽  
Vol 47 (1) ◽  
pp. 51-57 ◽  
Author(s):  
K.-O. Mosebach ◽  
W. Dirscherl
Keyword(s):  
Intraperitoneal Injection ◽  
Specific Activity ◽  
Initial Distribution ◽  
Male Rats ◽  
Initial Formation ◽  
Thigh Muscles ◽  
The Absolute ◽  
Cell Fractions

ABSTRACT The initial distribution of radioactive C was studied in the cell fractions of the liver, kidney, testes and thigh muscles after intraperitoneal injection of testosterone-4-14C into 40 day old male rats. To make this possible, the absolute and specific activity values (μc/mg C) were determined. After both ten and twenty minutes the cytoplasm fractions possessed the highest activity values, the only exception being the specific activity of the liver cytoplasm ten minutes after injection when the microsomes of the liver showed a higher activity. After 20 min the mitochondria possessed the highest specific activity values among the corpuscular fractions. The specific activity values in the microsomes of all four organs studied were lower 20 min after the time of injection than after 10 min, a fact, which is suspected to be the result of the initial formation of conjugates in the microsomes.

THE INFLUENCE OF VITAMIN C ON EOSINOPHIL RESPONSE TO ACUTE ALCOHOL INTOXICATION IN RATS

1960 ◽  
Vol XXXV (IV) ◽  
pp. 585-593 ◽  
Author(s):  
T. P. J. Vanha-Perttula
Keyword(s):  
Vitamin C ◽  
Ethyl Alcohol ◽  
Intraperitoneal Injection ◽  
Alcohol Intoxication ◽  
Albino Rats ◽  
Acute Alcohol Intoxication ◽  
Alcohol Administration ◽  
Initial Values

ABSTRACT The effect of ethyl alcohol on the circulating eosinophil cells has been studied in female albino rats. An intoxicating dose of alcohol caused a marked depletion of circulating eosinophils which was most clearly evident four hours after the administration of the alcohol. The initial values were not reached before 24 hours had elapsed. Intraperitoneal injection of vitamin C 12 hours prior to the alcohol administration very effectively prevented this eosinopenic reaction. The mechanism of regulation of the eosinophil cells in the circulation has been discussed in the light of previous results and of those obtained in this study.

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