scholarly journals Metabolomic Study of Polyamines in Rat Urine Following Intraperitoneal Injection of γ-Hydroxybutyric Acid

2018 ◽  
Author(s):  
Hyeon-Seong Lee ◽  
Chan Seo ◽  
Young-A Kim ◽  
Meejung Park ◽  
Boyeon Choi ◽  
...  
Keyword(s):  
Intraperitoneal Injection ◽  
Gas Chromatography Mass Spectrometry ◽  
Control Group ◽  
Hydroxybutyric Acid ◽  
Multivariate Statistical ◽  
Rat Urine ◽  
Metabolic Pattern ◽  
Star Pattern ◽  
Multiple Administration ◽  
Metabolomic Study

1) Background: Recently, illegal abuse of γ-hydroxybutyric acid (GHB) has increased in drug-facilitated crimes, but determination of GHB exposure and intoxication is difficult due to rapid metabolism of GHB. Its biochemical mechanism has not been completely investigated. And metabolomic study by polyamine profile and pattern analyses was not performed in rat urinefollowing intraperitoneal injection with GHB. 2) Methods: Polyamine profiling analysis by gas chromatography-mass spectrometry combined with star pattern recognition analysis was performed in this study. Multivariate statistical analysis was used to evaluate discrimination between control and GHB administration groups. 3) Results: Six polyamines were determined in control, single and multiple GHB administration groups. Star pattern showed distorted hexagonal shapes with characteristic and readily distinguishable patterns for each group. N1-Acetylspermine (p < 0.001), putrescine (p <0.006), N1-acetylspermidine (p <0.009), and spermine (p < 0.027) were significantly increased in single administration group but were significantly lower in the multiple administration group than in the control group. N1-Acetylspermine was the main polyamine for discrimination between control, single and multiple administration groups. Spermine showed similar levels in single and multiple administration groups. 4) Conclusions: The polyamine metabolic pattern was monitored in GHB administration groups. N1-Acetylspermine and spermine were evaluated as potential biomarkers of GHB exposure and addiction.

Monitoring of altered amino acid metabolic pattern in rat urine following intraperitoneal injection with γ-hydroxybutyric acid

Metabolomics ◽  
2018 ◽  
Vol 14 (9) ◽  
Author(s):  
Chan Seo ◽  
Myungjin Na ◽  
Jiyeun Jang ◽  
Meejung Park ◽  
Boyeon Choi ◽  
...  
Keyword(s):  
Amino Acid ◽  
Intraperitoneal Injection ◽  
Hydroxybutyric Acid ◽  
Rat Urine ◽  
Metabolic Pattern

Metabolomic study of polyamines in rat urine following intraperitoneal injection of γ-hydroxybutyric acid

Metabolomics ◽  
2019 ◽  
Vol 15 (4) ◽  
Author(s):  
Hyeon-Seong Lee ◽  
Chan Seo ◽  
Young-A Kim ◽  
Meejung Park ◽  
Boyeon Choi ◽  
...  
Keyword(s):  
Intraperitoneal Injection ◽  
Hydroxybutyric Acid ◽  
Rat Urine ◽  
Metabolomic Study

scholarly journals Metabonomics Study of Ginseng Glycoproteins on Improving Sleep Quality in Mice

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Ying Wang ◽  
Difu Zhu ◽  
Yinghong Chen ◽  
Ruizhi Jiang ◽  
Hong Xu ◽  
...  
Keyword(s):  
Sleep Quality ◽  
Brain Metabolism ◽  
Gas Chromatography Mass Spectrometry ◽  
Control Group ◽  
Glutamate Metabolism ◽  
Multivariate Statistical ◽  
Hormone Biosynthesis ◽  
The Relationship ◽  
Further Development

The changes of brain metabolism in mice after injection of ginseng glycoproteins (GPr) were analyzed by gas chromatography mass spectrometry- (GC/MS-) based metabolomics platform. The relationship between sedative and hypnotic effects of ginseng glycoproteins and brain metabolism was discussed. Referring to pentobarbital sodium subthreshold test, we randomly divided 20 mice into two groups: control and ginseng glycoproteins group. The mice from the control group were treated with normal saline by i.p and GPr group were treated with 60 mg/kg of GPr by i.p. The results indicated that GPr could significantly improve the sleep quality of mice. Through multivariate statistical analysis, we found that there were 23 differential metabolites in whole brain tissues between the control group and the GPr group. The pathway analysis exhibited that GPr may be involved in the regulation of the pathway including purine metabolism, nicotinate and nicotinamide metabolism, glycine, serine and threonine metabolism, arginine and proline metabolism, alanine, aspartate and glutamate metabolism, and steroid hormone biosynthesis. This work is helpful to understand the biochemical mechanism of GPr on promoting sleep and lay a foundation for further development of drugs for insomnia.

scholarly journals Metabonomic Evaluation of ZHENG Differentiation and Treatment by Fuzhenghuayu Tablet in Hepatitis-B-Caused Cirrhosis

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Shujun Sun ◽  
Jianye Dai ◽  
Wenyu Wang ◽  
Huijuan Cao ◽  
Junwei Fang ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Deficiency Syndrome ◽  
Gas Chromatography Mass Spectrometry ◽  
Control Group ◽  
Multivariate Statistical ◽  
Spleen Deficiency ◽  
Yin Deficiency ◽  
Plot Analysis ◽  
Zheng Differentiation ◽  
Tcm Syndrome

In Traditional Chinese Medicine (TCM), treatment based on ZHENG (also called TCM syndrome and pattern) differentiation has been applied for about 3 thousand years, while there are some difficulties to communicate with western medicine. In the present work, metabonomic methods were utilized to differentiate ZHENG types and evaluate the therapeutic efficiency of Fuzhenghuayu (FZHY) tablet in hepatitis-B-caused cirrhosis (HBC). Urine samples of 12 healthy volunteers (control group, CG) and 31 HBC patients (HBCG) were analyzed by gas chromatography mass spectrometry (GC/MS) and multivariate statistical analysis. The significantly changed metabolites between CG and HBCG were selected by PLS-DA loading plot analysis. Moreover, 4 ZHENGs were differentiated mutually, suggesting that there was urine metabolic material basis in ZHENG differentiation. The efficiency of FZHY tablet on subjects with spleen deficiency with dampness encumbrance syndrome (SDDES) and liver-kidney yin deficiency syndrome (LKYDS) was better than that of other syndromes. The efficiency of FZHY treatment based on ZHENG differentiation indicated that accurately ZHENG differentiating could guide the appropriate TCM treatment in HBC.

scholarly journals Salivary Metabolomics Fingerprint of Chronic Apical Abscess with Sinus Tract: A Pilot Study

2019 ◽  
Vol 2019 ◽  
pp. 1-6
Author(s):  
Noemi Montis ◽  
Elisabetta Cotti ◽  
Antonio Noto ◽  
Claudia Fattuoni ◽  
Luigi Barberini
Keyword(s):  
Sinus Tract ◽  
Polymorphonuclear Neutrophils ◽  
Gas Chromatography Mass Spectrometry ◽  
Control Group ◽  
Multivariate Statistical ◽  
Novel Approach ◽  
Metabolic Fingerprint ◽  
Multivariate Statistical Model ◽  
Liquefactive Necrosis ◽  
Healthy Control

Chronic apical abscess (CAA) is a lesion of apical periodontitis mostly characterized by areas of liquefactive necrosis with disintegrating polymorphonuclear neutrophils surrounded by macrophages. Its presence leads to local bacterial infection, systemic inflammatory response, pain, and swelling. The use of a novel approach for the study of CAA, such as metabolomics, seems to be important since it has proved to be a powerful tool for biomarkers discovery which could give novel molecular insight on CAA. So, the aim of this study was to verify the possibility to identify the metabolic fingerprint of CAA through the analysis of saliva samples. Nineteen patients were selected for this study: eleven patients affected by CAA with a sinus tract constituted the study group whereas eight patients without clinical and radiographic signs of CAA formed the healthy control group. Saliva samples were collected from each subject and immediately frozen at −80°C. Metabolomic profiles were obtained using a gas chromatography/mass spectrometry instrument. Subsequently, in order to compare the two groups, a multivariate statistical model was built that resulted to be statistically significant. The class of metabolites characterizing the CAA patients was closely related to the bacterial catabolism, tissue necrosis, and presence of a sinus tract. These preliminary results, for the first time, indicate that saliva samples analyzed by means of GC/MS metabolomics may be useful for identifying the presence of CAA, leading to new insights into this disease.

scholarly journals Non-targeted Serum Metabolomics Identifies Candidate Biomarkers Panels Associated with Nonalcoholic Fatty Liver Disease: A Pilot Study in Russian Male Patients

2021 ◽  
Vol 11 (1) ◽  
pp. 17-27 ◽  
Author(s):  
Elena V. Demyanova ◽  
Elena S. Shcherbakova ◽  
Tatyana S. Sall ◽  
Igor G. Bakulin ◽  
Timur Ya. Vakhitov ◽  
...  
Keyword(s):  
Liver Disease ◽  
Gas Chromatography Mass Spectrometry ◽  
Control Group ◽  
Hydroxybutyric Acid ◽  
Serum Samples ◽  
Metabolomics Data ◽  
Simple Steatosis ◽  
Functional Changes ◽  
Male Patients ◽  
Serum Metabolomics

Aims: The aim of the present study was to explore changes in the serum metabolome of patients with NAFLD relative to healthy controls to identify biomarkers associated with steatosis or Non-Alcoholic Steatohepatitis (NASH). Background: The serum metabolome reflects changes at the organismal level. This is especially important in Non-Alcoholic Liver Disease (NAFLD), where changes in hormones, cytokines, enzymes and other metabolic alterations can affect the liver, as well as adipose tissue, skeletal muscle and other systems. Objective: The objectives were to conduct non-targeted serum metabolomics, data processing, and identification of candidate biomarkers, as well as panels and assessment of their prognostic value. Materials and Methods: Non-targeted metabolomic analysis of blood serum samples from 21 male patients with NAFLD (simple steatosis or NASH) and seven male Control group was performed using gas chromatography-mass spectrometry. Results: A total of 319 serum metabolites were detected in NAFLD and Control groups, several of which differed significantly between groups. The most discriminating biomarkers were 3-hydroxybutyric acid, 2-hydroxybutyric acid, 2,3-dihydroxybutyric acid, arabitol and 3-methyl-2-oxovaleric acid. Using a panel of three, four or more markers could distinguish patients with NAFLD from controls, and patients with NASH from those with simple steatosis. Conclusion: We identified candidate biomarkers for simple steatosis and NASH. Since NAFLD is a multifactorial disease, it is preferable to use a marker panel rather than individual metabolites. Markers may not only result from dysregulation of metabolic pathways in patients with NAFLD, they may also reflect adaptive responses to disease, including functional changes in the intestinal microbiota.

scholarly journals A Gas Chromatography-Mass Spectrometry Based Study on Urine Metabolomics in Rats Chronically Poisoned with Hydrogen Sulfide

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Mingjie Deng ◽  
Meiling Zhang ◽  
Fa Sun ◽  
Jianshe Ma ◽  
Lufeng Hu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Hydrogen Sulfide ◽  
Gas Chromatography Mass Spectrometry ◽  
Control Group ◽  
Comprehensive Overview ◽  
Rat Urine ◽  
Tetradecanoic Acid ◽  
Chromatography Mass Spectrometry

Gas chromatography-mass spectrometry (GS-MS) in combination with multivariate statistical analysis was applied to explore the metabolic variability in urine of chronically hydrogen sulfide- (H2S-) poisoned rats relative to control ones. The changes in endogenous metabolites were studied by partial least squares-discriminate analysis (PLS-DA) and independent-samplest-test. The metabolic patterns of H2S-poisoned group are separated from the control, suggesting that the metabolic profiles of H2S-poisoned rats were markedly different from the controls. Moreover, compared to the control group, the level of alanine, d-ribose, tetradecanoic acid, L-aspartic acid, pentanedioic acid, cholesterol, acetate, and oleic acid in rat urine of the poisoning group decreased, while the level of glycine, d-mannose, arabinofuranose, and propanoic acid increased. These metabolites are related to amino acid metabolism as well as energy and lipid metabolismin vivo. Studying metabolomics using GC-MS allows for a comprehensive overview of the metabolism of the living body. This technique can be employed to decipher the mechanism of chronic H2S poisoning, thus promoting the use of metabolomics in clinical toxicology.

scholarly journals Identification of Morphine and Heroin-Treatment in Mice Using Metabonomics

Metabolites ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 607
Author(s):  
Wuhuan Lu ◽  
Ran Zhang ◽  
Wei Sheng ◽  
Luohua Feng ◽  
Peng Xu ◽  
...  
Keyword(s):  
Citric Acid ◽  
Oxalic Acid ◽  
Tca Cycle ◽  
Serum Levels ◽  
Gas Chromatography Mass Spectrometry ◽  
Hydroxybutyric Acid ◽  
Metabolic Pattern ◽  
Opiate Abuse ◽  
Aconitic Acid

Although heroin and morphine are structural analogues and morphine is a metabolite of heroin, it is not known how the effect of each substance on metabolites in vivo differs. Heroin and morphine were administered to C57BL/6J mice in increasing doses from 2 to 25 and 3 to 9 mg kg−1 (twice a day, i.p.), respectively, for 20 days. The animals underwent withdrawal for 5 days and were readministered the drugs after 10 days. Serum and urine analytes were profiled using gas chromatography-mass spectrometry (GC-MS), and metabolic patterns were evaluated based on metabonomics data. Metabonomics data showed that heroin administration changed metabolic pattern, and heroin withdrawal did not quickly restore it to baseline levels. A relapse of heroin exposure changed metabolic pattern again. In contrast, although the administration of morphine changed metabolic pattern, whether from morphine withdrawal or relapse, metabolic pattern was similar to control levels. The analysis of metabolites showed that both heroin and morphine interfered with lipid metabolism, the tricarboxylic acid (TCA) cycle and amino acid metabolism. In addition, both heroin and morphine increased the levels of 3-hydroxybutyric acid and citric acid but decreased the serum levels of 2-ketoglutaric acid and tryptophan. Moreover, heroin and morphine reduced the levels of aconitic acid, cysteine, glycine, and oxalic acid in urine. The results show 3-Hydroxybutyric acid, tryptophan, citric acid and 2-ketoglutaric acid can be used as potential markers of opiate abuse in serum, while oxalic acid, aconitic acid, cysteine, and glycine can be used as potential markers in urine.

Metabolic Alteration in Plasma and Biopsies From Patients With IBD

2021 ◽  
Author(s):  
Maria Laura Santoru ◽  
Cristina Piras ◽  
Federica Murgia ◽  
Vera Piera Leoni ◽  
Martina Spada ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Ulcerative Colitis ◽  
Gas Chromatography Mass Spectrometry ◽  
Control Group ◽  
Therapeutic Approaches ◽  
Energetic Metabolism ◽  
Multivariate Statistical ◽  
Bowel Diseases ◽  
Inflammatory Bowel ◽  
Potential Tool

Abstract Background Inflammatory bowel diseases (IBD) are chronic inflammatory disorders of the gastrointestinal tract, with periods of latency alternating with phases of exacerbation, and include 2 forms: Crohn disease (CD) and ulcerative colitis (UC). Although the etiology of IBD is still unclear, the identification and understanding of pathophysiological mechanisms underlying IBD could reveal newly targeted intestinal alterations and determine therapeutic approaches. Methods In this study, by using gas chromatography-mass spectrometry, we characterized plasma and biopsies from the metabolomics profiles of patients with IBD compared with those of a control group. Results The results showed a different metabolomics profile between patients with CD (n = 50) and patients with UC (n = 82) compared with the control group (n = 51). Multivariate statistical analysis of the identified metabolites in CD and UC showed changes in energetic metabolism, and lactic acid and ornithine in particular were altered in both plasma and colon biopsies. Moreover, metabolic changes were evidenced between the normal ileum and colon tissues. These differences disappeared when we compared the inflamed ileum and colon tissues, suggesting a common metabolism. Conclusions This study showed how the metabolomics profile could be a potential tool to identify intestinal alterations associated with IBD and may have application in precision medicine and for better defining the pathogenesis of the disease.

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