Start-up of sequencing batch reactor with Thiosphaera pantotropha for treatment of high-strength nitrogenous wastewater and sludge characterization

2016 ◽  
Vol 23 (20) ◽  
pp. 20065-20080 ◽  
Author(s):  
Pranita S. Phatak ◽  
Saurabh Trivedi ◽  
Anurag Garg ◽  
Sudhir K. Gupta ◽  
Suparna Mukherji
2002 ◽  
Vol 46 (11-12) ◽  
pp. 343-348 ◽  
Author(s):  
W.J. Ng ◽  
S.L. Ong ◽  
K.Y. Tan ◽  
J.Y. Hu ◽  
L.Y. Lee ◽  
...  

Addition of glucose as the co-substrate and the use of a water-based polymer to enhance granulation were studied with the aim of shortening the start-up period of an anSBR (anerobic sequencing batch reactor) for high-strength industrial wastewater treatment. An initial start-up feed composition consisting of 70% sucrose and 30% industrial wastewater was favorable to reduce the start-up time. Increase in the average particle size and reduction in settled supernatant turbidity was observed with increased water-based polymer addition up to a concentration of 0.1 m/L. Polymer dosages above 0.1 ml/L were undesirable as big and “flaky” flocs were formed which prevented the flocs from settling quickly. Addition of 0.005 ml/L to 0.1 ml/L polymer to the mixed liquor had no observable adverse effects on the anaerobic biomass metabolism. Instead, the addition of the polymer appeared to improve biogas production and had an overall positive effect on anSBR performance treating the industrial wastewater.


2009 ◽  
Vol 60 (4) ◽  
pp. 1049-1054 ◽  
Author(s):  
S. López–Palau ◽  
J. Dosta ◽  
J. Mata-Álvarez

Aerobic granular sludge was cultivated in a sequencing batch reactor (SBR) in order to remove the organic matter present in winery wastewater. The formation of granules was performed using a synthetic substrate. The selection parameter was the settling time, as well as the alternation of feast-famine periods, the air velocity and the height/diameter ratio of the reactor. After 10 days of operation under these conditions, the first aggregates could be observed. Filamentous bacteria were still present in the reactor but they disappeared progressively. During the start-up, COD loading was increased from 2.7 to 22.5 kg COD/(m3 day) in order to obtain a feast period between 30 and 60 minutes. At this point, granules were quite round, with a particle diameter between 3.0 and 4.0 mm and an average density of 6 g L−1. After 120 days of operation, synthetic media was replaced by real winery wastewater, with a COD loading of 6 kg COD/(m3 day). The decrease of the organic load implied a reduction of the aggregate diameter and a density increase up to 13.2 g L−1. The effluent was free of organic matter and the solids concentration in the reactor reached 6 g VSS L−1.


2012 ◽  
Vol 66 (3) ◽  
pp. 543-548
Author(s):  
Binbin Wang ◽  
Shunlian Liu ◽  
Hongmei Zhao ◽  
Xinyan Zhang ◽  
Dangcong Peng

Variations of extracellular polymeric substances (EPS) and its components with sludge granulation were examined in a lab-scale sequencing batch reactor (SBR) which was fed with sodium nitrate and sodium acetate. Ultrasonication plus cation exchange resin (CER) were used as the EPS extraction method. Results showed that after approximately 90 d cultivation, the sludge in the reactor was almost granulated. The content of extracellular polysaccharides increased from 10.36 mg/g-VSS (volatile suspended solids) at start-up with flocculent sludge to 23.18 mg/g-VSS at 91 d with matured granular sludge, while the content of extracellular proteins were almost unchanged. Polysaccharides were the major components of EPS in anoxic granular sludge, accounting for about 70.6–79.0%, while proteins and DNA accounted for about 16.5–18.9% and 4.6–9.9%, respectively. It is proposed that EPS play a positive role in anoxic sludge granulation and polysaccharides might be strongly involved in aggregation of flocs into granules.


2012 ◽  
Vol 65 (5) ◽  
pp. 840-844 ◽  
Author(s):  
D. Wu ◽  
T. Hao ◽  
H. Lu ◽  
H. K. Chui ◽  
M. C. M. van Loosdrecht ◽  
...  

This study explored a sulfur cycle-associated biological phosphorus (P) removal process in a covered and non-aerated sequencing batch reactor (SBR) fed with volatile fatty acid (VFA) and sulfate separately. During the 60-day start-up, both phosphate release and uptake rates increased, while poly-phosphate cyclically increased and decreased accordingly. The P-release and P-uptake rates were associated with VFA uptake and sulfate reduction. The average ratio of potassium to phosphate during the P-uptake and P-release was also determined to be 0.29–0.31 mol K/mol P, which is close to a reported value (0.33) for biological phosphorus removal. All this evidence confirmed there was biological P removal in this reactor, in which metabolism could be different from conventional biological P removal.


2015 ◽  
Vol 295 ◽  
pp. 153-160 ◽  
Author(s):  
Xiaodong Liu ◽  
Yan Chen ◽  
Xin Zhang ◽  
Xinbai Jiang ◽  
Shijing Wu ◽  
...  

2004 ◽  
Vol 50 (10) ◽  
pp. 243-249 ◽  
Author(s):  
L.Y. Lee ◽  
J.Y. Hu ◽  
S.L. Ong ◽  
W.J. Ng ◽  
J.H. Ren ◽  
...  

A two-stage sequencing batch reactor (SBR) system was used for treatment of oily wastewater with COD and oil and grease (O&G) concentrations ranging from 1,722–7,826 mg/L and 5,365–13,350 mg/L, respectively. A suitable start-up protocol was developed using gradual increase in oily wastewater composition with methanol as the co-substrate. This strategy enabled a short acclimation period of 12 days for the sludge in the two-stage SBR to adapt to the oily wastewater. After acclimation, the 1st stage and 2nd stage SBRs were able to achieve COD removals of 47.0 ± 2.4% and 95.3 ± 0.5%, respectively. The 1st stage SBR was able to achieve 99.8 ± 0.1% of O&G removal and effluent O&G from the 1st stage SBR was only 6 ± 2 mg/L. The 2nd stage SBR was used to further remove COD in the effluent from the 1st stage SBR. The final effluent from the 2nd stage SBR had a COD concentration of 97 ± 16 mg/L with no detectable O&G content. Thus, a two-stage SBR system was shown to be feasible for treating high strength oily wastewater to meet the local discharge standards.


2012 ◽  
Vol 610-613 ◽  
pp. 1454-1458
Author(s):  
Ming Fen Niu ◽  
Hong Jing Jiao ◽  
Li Xu ◽  
Yan Yu ◽  
Jian Wei

A2N is two-sludge system, by using the method that first bringing up the cultivation of denitrifying phosphorus removing bacteria (DPB) and nitrification biofilm separately then connecting them, which can start up A2N system successfully. Nitrification biofilm was cultivated in a sequencing batch reactor (SBR). After 30 days, NH4+-N effluent concentration steadily stayed below 0.5mg·L-1.In another SBR, the activated sludge for the enrichment of DPB is from the anaerobic tank, which was firstly operated under anaerobic/aerobic (A/O) condition. After 20 days, PAOs was successfully enriched. Then, the activated sludge was conducted under anaerobic/anoxic/aerobic (A/A/O) condition, maintaining the anaerobic time, gradually increased anoxic time and induced aerobic time. After 30 days DPB was successfully enriched, two phases totally take 50 days. The removal efficiency of total nitrogen and phosphorus are above 85 % and 95 %, so that A2N system was started up successfully.


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