Effects of heavy metals Cd2+, Pb2+ and Zn2+ on DNA damage of loach Misgurnus anguillicaudatus

2008 ◽  
Vol 3 (1) ◽  
pp. 50-54 ◽  
Author(s):  
Yingmei Zhang ◽  
Yejing Wang ◽  
Runliu Yu ◽  
Sheng Zhang ◽  
Zhenbin Wu
Keyword(s):  
Heavy Metals ◽  
Dna Damage ◽  
Misgurnus Anguillicaudatus

CHAPTER 27. Heavy Metals – Lead, Mercury and Cadmium and Their Impact on DNA Damage Measured by the Micronucleus Assay

2019 ◽  
pp. 435-449
Author(s):  
Armen Nersesyan ◽  
Michael Kundi ◽  
Miroslav Mišík ◽  
Georg Wultsch ◽  
Siegfried Knasmueller
Keyword(s):  
Heavy Metals ◽  
Dna Damage ◽  
Micronucleus Assay

Blood heavy metals and DNA damage among children living in an informal E-waste processing area in Vietnam

2020 ◽  
pp. 1-19 ◽  
Author(s):  
Hien Thi Thu Ngo ◽  
Li Liang ◽  
Diep Bich Nguyen ◽  
Hai Ngoc Doan ◽  
Pensri Watchalayann
Keyword(s):  
Heavy Metals ◽  
Dna Damage ◽  
Waste Processing

scholarly journals Genotoxicity Study with Special Reference to Comet Test in the Blood Cells of Workers Exposed to Sewage Water

2014 ◽  
Vol 2014 ◽  
pp. 1-7
Author(s):  
Rajlaxmi Basu ◽  
Soumendra Nath Talapatra ◽  
Aniruddha Mukhopadhyay ◽  
Moumit Roy Goswami ◽  
Siddhartha Sankar Ray ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Dna Damage ◽  
Blood Cells ◽  
Adaptive Response ◽  
Suspended Solids ◽  
Total Suspended Solids ◽  
Total Dissolved Solids ◽  
Sewage Water ◽  
Total Iron ◽  
Aged Group

Awareness among sewage workers to occupational exposure is growing slowly in many developing countries. Lead (Pb) and cadmium (Cd) are present in sewage water and workers are exposed to these metals as a result of unprotected handling. These heavy metals exposures are responsible for DNA damage and lowering blood total iron (Fe) concentration. Zinc (Zn) is an element for promoting metallothionine expression and binds the free Cd. The total suspended solids (TSS), total dissolved solids (TDS), Pb, and Cd were estimated in sewage water. The whole blood Zn and Fe concentration and Pd and Cd were also estimated. Genotoxicity as indicated by DNA damage was studied by comet assay. It was observed that there were significant differences (P<0.001) of Pb and Cd concentration in blood for the sewage workers when compared with control population. DNA damage was also observed to be significantly (P<0.001) higher in the exposed groups but their blood Fe concentration was significantly lower, which may be the reason for their tendency for retention of blood Cd and make them more susceptible. This study also indicated that aged workers had higher blood Zn concentrations as compared to the younger (working < 20 years) workers. This may indicate a possible adaptive response. The present study proposes that younger (working < 20 years) group is more susceptible as compared to aged group (working > 20 years).

Blood Concentrations of Heavy Metals and DNA Damage Levels of Southeast Asia Female Immigrants

Epidemiology ◽  
2007 ◽  
Vol 18 (Suppl) ◽  
pp. S22
Author(s):  
W T Wu ◽  
T E Liu ◽  
S H Liou ◽  
T N Wu
Keyword(s):  
Heavy Metals ◽  
Dna Damage ◽  
Southeast Asia ◽  
Blood Concentrations ◽  
Female Immigrants

Abstract 39: Exposure to heavy metals and polycyclic aromatic hydrocarbons and DNA damage in Taiwanese traffic conductors.

2012 ◽  
Vol 21 (11 Supplement) ◽  
pp. 39-39
Author(s):  
Han-Bin Huang ◽  
Guan-Wen Chen ◽  
Chien-Jen Wang ◽  
Yong-Yang Lin ◽  
Saou-Hsing Liou ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Dna Damage ◽  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Polycyclic Aromatic

Comet Assay: A Strong Tool for Evaluating DNA Damage and Comprehensive Guidelines for Plant Cells

2017 ◽  
Vol 3 (02) ◽  
pp. 65-72
Author(s):  
Ashish Agnihotri ◽  
Chandra Shekhar Seth
Keyword(s):  
Heavy Metals ◽  
Dna Damage ◽  
Comet Assay ◽  
Single Cells ◽  
Strand Breakage ◽  
Dna Strand ◽  
Environmental Genotoxicity ◽  
Genetic Ecotoxicology ◽  
Detailed Protocol ◽  
Dna Strand Breakage

Heavy metals affect plant system in various toxic ways including morphophysiological alterations and genotoxic damages inside a plant cell. The extent of DNA damage under any genotoxic agents can be effectively measured in single cells applying comet assay approach. Comet assay primarily measures DNA strand breakage in single cells and its use has increased in different areas: clinical applications, human monitoring, radiation biology and genetic ecotoxicology. This paper is a review of the detailed protocol and precautions to be taken while performing comet assay and may have been slightly modified from other original protocols according to the plant, organ, cell type, etc. In conclusion, the study reviewed in this paper demonstrate that the comet assay application in plants provides a reliable, sensitive and rapid system for the study of environmental genotoxicity caused by heavy metals.

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