scholarly journals Genotoxicity Study with Special Reference to Comet Test in the Blood Cells of Workers Exposed to Sewage Water

2014 ◽  
Vol 2014 ◽  
pp. 1-7
Author(s):  
Rajlaxmi Basu ◽  
Soumendra Nath Talapatra ◽  
Aniruddha Mukhopadhyay ◽  
Moumit Roy Goswami ◽  
Siddhartha Sankar Ray ◽  
...  

Awareness among sewage workers to occupational exposure is growing slowly in many developing countries. Lead (Pb) and cadmium (Cd) are present in sewage water and workers are exposed to these metals as a result of unprotected handling. These heavy metals exposures are responsible for DNA damage and lowering blood total iron (Fe) concentration. Zinc (Zn) is an element for promoting metallothionine expression and binds the free Cd. The total suspended solids (TSS), total dissolved solids (TDS), Pb, and Cd were estimated in sewage water. The whole blood Zn and Fe concentration and Pd and Cd were also estimated. Genotoxicity as indicated by DNA damage was studied by comet assay. It was observed that there were significant differences (P<0.001) of Pb and Cd concentration in blood for the sewage workers when compared with control population. DNA damage was also observed to be significantly (P<0.001) higher in the exposed groups but their blood Fe concentration was significantly lower, which may be the reason for their tendency for retention of blood Cd and make them more susceptible. This study also indicated that aged workers had higher blood Zn concentrations as compared to the younger (working < 20 years) workers. This may indicate a possible adaptive response. The present study proposes that younger (working < 20 years) group is more susceptible as compared to aged group (working > 20 years).

Author(s):  
R. Sandhiya ◽  
K. Sumaiya Begum ◽  
D. Charumathi

<p><strong>Objective: </strong>The objectives of the present study were a) to isolate and screen bacteria for dye removal from synthetic solution b) to optimize various variables such as pH, static/shaking and initial dye concentration on degradation of triphenyl methane dyes namely basic violet 3 and basic green 4 by isolated <em>Staphylococcus aureus</em> c) to analyse enzymes involved in the biodegradation of triphenylmethane dyes d) to treat real leather dyeing wastewater with newly isolated strain of <em>Staphylococcus aureus </em>e) to characterize untreated and treated leather dyeing wastewater f) to study the effects of real and treated effluent on plants and <em>Rhizobium</em>.<strong></strong></p><p><strong>Methods: </strong>Isolation of bacteria from sludge was carried out by spread plate method and the bacteria was identified by morphological and biochemical characterization. The isolated bacterium was screened for dye decolorization potential of triphenylmethane dyes basic violet 3 and basic green 4 The effects of parameters were studied by varying pH (from 3 to 9), temperature (from 15-45 °C), and initial dye concentration (from 10-500 mg/l). The enzyme involved in biodegradation was studied in intracellular extract. Real leather dyeing wastewater was treated with the bacteria and characterized. The treated wastewater was tested on plants and <em>Rhizobium </em>for toxicity. <strong></strong></p><p><strong>Results: </strong>Dye decolorization potential of bacteria <em>Staphylococcus aureus</em> isolated from wastewater for leather dyes basic violet 3 and basic green 4 were evaluated. Dye decolorization using bacteria was found to be dependent on physicochemical parameters (shaking, pH and initial dye concentration). Enzymes NADH-DCIP reductase and MG reductase were found to play dominant role during biodegradation of synthetic dyes. Application oriented studies using growing bacteria in pure cultures were carried out with leather dyeing wastewater collected from DKS prime tanners. Analysis of raw leather dyeing wastewater showed high pollution load in terms of color, Total solids, Total suspended solids, Total dissolved solids and Biological oxygen demand whereas the leather dyeing wastewater treated with pure culture of <em>Staphylococcus aureus</em> showed considerable decrease in Total solids, Total suspended solids, Total dissolved solids and Biological oxygen demand values which were within the permissible limits. Phytotoxicity and microbial toxicity studies confirmed the non-toxic nature of treated leather dyeing wastewater. <strong></strong></p><p><strong>Conclusion: </strong>Our study proved that <em>Staphylococcus aureus</em> can serve as a potential remediation agent for the treatment of leather dyeing wastewater.</p>


2019 ◽  
Vol 6 (1) ◽  
pp. 45-54 ◽  
Author(s):  
Veymar G. Tacias-Pascacio ◽  
Abumalé Cruz-Salomón ◽  
José H. Castañón-González ◽  
Beatriz Torrestiana-Sanchez

Background: Wet coffee processing consists of the removal of the pulp and mucilage of the coffee cherry. This process generates a large amount of acidic wastewater which is very aggressive to the environment because of its high content of recalcitrant organic matter. Therefore, treatment is necessary before discharge to water bodies. Because of this reason, this study aimed to evaluate the organic matter removal efficiency in an Anaerobic Baffled Bioreactor (ABR) coupled to a Microfiltration Membrane (MF) system as a new eco-friendly option in the treatment of wet Coffee Processing Wastewater (CPWW). Methods: Two systems (S1 and S2) were evaluated at Hydraulic Retention Times (HRT) of 59 h and 83 h, respectively. Both systems were operated at mesophilic conditions, at a Transmembrane Pressure (TMP) of 50 kPa during 1800 h. Results: The S2 generated higher organic matter removal efficiency, reaching removal values of turbidity of 98.7%, Chemical Oxygen Demand (COD) of 81%, Total Solids (TS) of 72.6%, Total Suspended Solids (TSS) of 100%, and Total Dissolved Solids (TDS) of 61%, compared with the S1. Conclusion: The S2 represents a new eco-friendly alternative to treat CPWW and reduce its pollutant effect.


2009 ◽  
Vol 6 (3) ◽  
pp. 909-914 ◽  
Author(s):  
K. Karunakaran ◽  
P. Thamilarasu ◽  
R. Sharmila

Groundwater samples collected at different locations in and around Namakkal were analyzed for their physicochemical characteristics. Ten locations of groundwater samples were collected and studied for every two month for the period June-2007 to December-2007. The present investigation is focused on the determination of physicochemical parameters such as temperature, taste, turbidity, electrical conductivity, pH, hardness, total solids, total dissolved solids, total suspended solids, chlorides, sulphate, nitrate, fluorides, dissolved oxygen, sodium, potassium andE.colibacterium. Groundwater suitability for domestic and irrigation purposes was examined by using WHO and BIS standards, which indicate the groundwater in a few areas, were not much suitable for domestic and agriculture purposes. Thus the objective of this study is to identify the quality of groundwater especially in the town and near by town where groundwater is used for domestic and agriculture purposes is discussed.


2008 ◽  
Vol 273-276 ◽  
pp. 770-775 ◽  
Author(s):  
Teresa Castelo-Grande ◽  
Paulo A. Augusto ◽  
P. Monteiro ◽  
Domingos Barbosa

In this work the use of Membrane Bioreactors to treat leachate effluents is discussed. The problem of membrane fouling is addressed, and some of the efforts being done to overcome this problem presented. The process optimization requires the reduction of other parameters, some directly related to mass transfer, such as the total amount of solids, the total dissolved solids and the total suspended solids. The preliminary results obtained show the high potential of this technique for the treatment of leachates, mainly for the reduction of solids in leachate streams.


2017 ◽  
Vol 13 (3) ◽  
pp. 167
Author(s):  
Husnah Husnah ◽  
Eko Prianto ◽  
Siti Nurul Aida

Sungai Musi merupakan sungai besar mengaliri wilayah Sumatera Selatan, Lampung, dan Bengkulu , dan bervariasi dalam pemanfaatannya, khususnya di bagian hilir, didominasi oleh kegiatan industri yang membuang limbahnya ke Sungai Musi. Kajian pengaruh industri terhadap Sungai Musi telah dilakukan, namun sebatas analisis fisik dan kimia lingkungan dan belum mengarah kepada pengaruhnya terhadap organisme air. Organisme air adalah indikator penting perubahan lingkungan karena organisme khususnya organisme dasar (benthos) menyimpan sejarah proses-proses terjadi di perairan. Riset yang bertujuan untuk mengetahui kualitas perairan Sungai Musi bagian hilir ditinjau dari karakteristik fisik dan kimia dan struktur makrozoobenthos telah dilakukan di Sungai Musi, Sumatera Selatan pada bulan Mei dan September 2006. Riset dilakukan bersifat survei lapangan. Delapan stasiun ditentukan di Sungai Musi bagian hilir berdasarkan pada perbedaan mikrohabitat. Stasiun riset masing masing antara lain Sejagung, Pulokerto, Jembatan Ampera, Sebokor, Pulau Burung, Upang, Pulau Payung, dan Sungsang. Pada masing masing stasiun, dilakukan pengambilan contoh air untuk parameter fisika, kimia, dan makrobenthos. Contoh air diambil dari atas perahu motor pada kedalaman 1,0 m dari permukaan air dengan menggunakan kemmerer water sampler. Sebagian contoh dianalisis di lapangan (suhu, pH, dan oksigen terlarut) dan sebagian lagi yaitu jumlah padatan tersesuspensi (total suspended solids), jumlah padatan terlarut (total dissolved solids), jumlah karbon organik (total organic carbon), organik karbon terlarut (dissolved organic carbon), konsumsi oksigen biologi (biochemical oxygen demand), nitrat, dan fosfat dianalisis di laboratorium kimia. Contoh makrozoobenthos diambil pada 10 titik di masing-masing stasiun, dengan menggunakan ekman dredge dengan bukaan mulut 400 cm2. Contoh makrobenthos pada masingmasing titik tersebut disortir dengan menggunakan saringan dan kemudian digabungkan (dikomposit) dan diawetkan dengan formalin 10%. Data kualitas air dianalisis dengan principle component analysis dan kelimpahan makrozoobenthos dianalisis dengan analisis cluster. Kualitas perairan di Sungai Musi bagian hilir dikelompokkan atas 2 yang mengalami tekanan berat yaitu dari Sejagung sampai dengan Pulau Burung dan tekanan ringan yaitu dari Upang sampai dengan Muara Sungai Musi. Kelompok pertama dicirikan oleh nilai konsentrasi total dissolved solids, total organic carbon, dan dissolved organic carbon yang tinggi diiringi dengan kelimpahan makrozoobenhthos yang rendah serta didominasi oleh Tubifex sp. Kelompok ke-2 dari Upang sampai dengan Muara Sungai Musi dicirikan oleh nilai konsentrasi total suspended solids yang tinggi, dengan kelimpahan makrozoobenthos yang tinggi dan didominasi oleh Gammarus. Musi River is a large river , crossing three provinces, South Sumatra, Lampung and Bengkulu, and differeing in types and levels of its resources ultization, particularly at the down stream of Musi River, mostly dominated by industries activities producing a waste which flows to the river. Several studies on the effect of industries on the Musi River have been conducted , however , limmieted on physical dan chemical aspects of the water, not yet to evaluate its effect on aquatic organism. Aquatic organism such macrozoobenthos is important indicator of environmental changes since this organism records the history of processes occurred in the water. Study to assess water quality of the down stream Musi River based on physical, chemical water characteristics and macrozoobenthos community structure was conducted at may and september 2006 in Musi River located in South Sumatera Province of Indonesia. The study used inventory field survey. Eight sampling sites; Sejagung, Pulokerto, Jembatan Ampera, Sebokor, Pulau Burung, Upang, Pulau Payung, and Sungsang were selected based on the microhabitat difference. water sampling for physical and chemical parameters and sediment, and  macrozoobenthos were carried in each sampling site. Water sample was collected at a depth of 1.0 m from the water surface by using kemmerer water sampler. Some water quality parameters such as temperature, pH, and dissolved oxygen) were directly analyzed in the field, while the others such as total suspended solids, total dissolved solids, total organic carbon, dissolved organic carbon, biochemical oxygen demand, nitrate, and phosphate were analyzed in laboratory. Macrozoobenthos was collected at ten sampling points in each sampling sites using Ekman Dredge of 400 cm2 mouth opening. Macrozoobenthos from ten sampling points was composited, sorted and preserved with formalin 10%. Water quality parameters were analyzed with principle component analysis while macrozoobenthos abundance was analyzed with cluster. Results revealed that water quality at the down stream Musi River was classified into two groups. The first group was the heavy degraded sites from Sejagung to Pulau Burung, characterized by having high concentration of total dissolved solids, total organic carbon, and dissolved organic carbon, low abundance of macrozoobenthos with Tubifex sp. as the dominant species. The second group was light degraded sites from Upang to the mouth of Musi River, characterized by high concentration of total suspended solids and high macrozoobenthos abundance with Gammarus sp. as the dominant species.


2021 ◽  
Vol 49 (2) ◽  
pp. 257-265
Author(s):  
Jakia Hasan ◽  
Md Mozzammel Hoque ◽  
Ahmed Fazley Rabbi ◽  
Shafiqur Rahman ◽  
Md Zulfikar Ali

Potential spat collectors and suitable culture sites were investigated for the development of edible oyster culture from June to December 2019 at three study sites (Moheshkhali, the Jeti of Nuniarchora, and Sonadia) of Cox’s Bazar coast of Bangladesh. Four types of collectors such as oyster shell, earthen pot, kortal (windowpane oyster shell) and tiles were investigated. On site sampling of water quality parameters and number of spats on each collector were done at 15-days interval following standard methods. Results indicated insignificant difference (P > 0.05) in temperature, salinity, dissolved oxygen and pH, while significant difference (P < 0.05) in total dissolved solids, total suspended solids and Chl-a content of the water among the study locations. However, these parameters were in suitable range for growth and spawning of oyster. Results also indicated spat recruitment was significantly influenced by the types of collectors and study locations with significantly (P < 0.05) higher spat were recruited in Tiles. Tiles were also found to facilitate with an advantage of recycling of this collector. The location in interaction with polluting materials such as total dissolved solids and total suspended solids had a significant effect on spat recruitment rate and therefore, Sonadia was the most suitable location for spat recruitment. This study site can also be used as a suitable location for the development of oyster culture in the cost of Bay of Bengal, Bangladesh based on its environmental characteristics. Bangladesh J. Zool. 49 (2): 257-265, 2021


PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0257715
Author(s):  
Simon Appah Aram ◽  
Benjamin M. Saalidong ◽  
Patrick Osei Lartey

The occurrence of pollution indicator bacteria (total and faecal coliform) has been used as a sanitary parameter for evaluating the quality of drinking water. It is known that these indicators are associated with disease causing organisms which are of great concern to public health. This study assessed the relationship between coliform bacteria and water geochemistry in surface and ground water systems in the Tarkwa mining area using logistic regression models. In surface water sources, higher values of chloride (OR = 0.891, p<005), phosphates (OR = 0.452, p<0.05), pH (OR = 0.174, p<0.05) and zinc (OR = 0.001, p<0.05) were associated with lower odds of faecal coliform contamination. In groundwater sources, higher values of phosphates (OR = 0.043, p<0.001), total dissolved solids (OR = 0.858, p<0.05), turbidity (OR = 0.996, p<0.05) and nickel (OR = 6.09E-07, p<0.05) implied non-contamination by faecal coliform. However, higher values of electrical conductivity (OR = 1.097, p<0.05), nitrates (OR = 1.191, p<0.05) and total suspended solids (OR = 1.023, p<0.05) were associated with higher odds of faecal coliform contamination of groundwater sources. Nitrates and total suspended solids, in this case, were completely mediated by the heavy metals. For total coliform in surface water systems, higher values of magnesium (OR = 1.070, p<0.05) was associated with higher odds of total coliform contamination while higher values of phosphates (OR = 0.968, p<0.05) was associated with lower odds of total coliform contamination although the presence of heavy metals completely mediated these relationships. For ground water systems, higher values of pH (OR = 0.083, p<0.05), phosphates (OR = 0.092, p<0.05), turbidity (OR = 0.950, p<0.05) and chloride (OR = 0.860, p<0.05) were associated with lower odds of total coliform contamination. However, higher values of total suspended solids (OR = 1.054, p<0.05) and nitrates (OR = 1.069, p<0.05) implied contamination of total coliform in ground water sources. The relationship between nitrates and total coliform were mediated by the heavy metals. This study establishes the need to monitor, manage and remediate surface and ground water sources for potential disease causing microbes in ways that takes into consideration the factors that create different conditions in the two water systems. This study validates the usefulness of statistical models as tools for preventing surface and ground water contamination.


2010 ◽  
Vol 113-116 ◽  
pp. 1726-1731
Author(s):  
Wei Xu ◽  
Li Fen Hao ◽  
Lei Zhou

Traditional lime and sulfide dehairing process is a major source of the pollution, such as biochemical oxygen demand (BOD), chemical oxygen demand (COD), total dissolved solids (TDS), total suspended solids (TSS) and toxic H2S gas, etc. Enzymatic dehairing process is known to be cleaner but its dehairing effects are unsatisfactory. Hence, an attempt has been made to solve those two unhairing methods’ disadvantages through a biochemical approach. Several commercial enzyme formulations were chosen to research effects of hydrosulfide and peroxide on its activities, and then hair-saving enzymatic unhairing experiments with those two reagents were conducted in paste and pile method more effectively and cleanly. Results show that activity of enzyme 2709 is stabilized by the addition of hydrosulfide through activity measurements; Peroxide has some activation effect on protease 3942. Hair removal is found to be complete using scanning electron microscope (SEM) analysis. Those two processes enjoy a significant reduction in BOD, COD, TDS and TSS. The perfromances of the experimental leathers are also comparable to that of conventional ones. Therefore, those are cleaner processing technologies that could be chosen to solve traditional method’s disadvantage.


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