CHAPTER 27. Heavy Metals – Lead, Mercury and Cadmium and Their Impact on DNA Damage Measured by the Micronucleus Assay

2019 ◽  
pp. 435-449
Author(s):  
Armen Nersesyan ◽  
Michael Kundi ◽  
Miroslav Mišík ◽  
Georg Wultsch ◽  
Siegfried Knasmueller
Keyword(s):  
Heavy Metals ◽  
Dna Damage ◽  
Micronucleus Assay

Effects of heavy metals Cd2+, Pb2+ and Zn2+ on DNA damage of loach Misgurnus anguillicaudatus

2008 ◽  
Vol 3 (1) ◽  
pp. 50-54 ◽  
Author(s):  
Yingmei Zhang ◽  
Yejing Wang ◽  
Runliu Yu ◽  
Sheng Zhang ◽  
Zhenbin Wu
Keyword(s):  
Heavy Metals ◽  
Dna Damage ◽  
Misgurnus Anguillicaudatus

scholarly journals Effect of High-Dose Topical Minoxidil on Erythrocyte Quality in SKH1 Hairless Mice

Animals ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 731
Author(s):  
Eduardo Naranjo-Vázquez ◽  
María Guadalupe Sánchez-Parada ◽  
Belinda Claudia Gómez-Meda ◽  
Ana Lourdes Zamora-Perez ◽  
Martha Patricia Gallegos-Arreola ◽  
...  
Keyword(s):  
Dna Damage ◽  
Peripheral Blood ◽  
Micronucleus Assay ◽  
Control Group ◽  
High Dose ◽  
Entire Body ◽  
Hairless Mice ◽  
Mutagenic Potential ◽  
Polychromatic Erythrocytes ◽  
High Doses

SKH1 hairless mice are widely used in carcinogenesis and dermatology research due to their bare skin, as exposure to different agents is facilitated. Minoxidil is a cosmetic drug that is recognized as a mitogenic agent, and mitogens are suggested to have carcinogenic and mutagenic potential by inducing cell division and increasing the possibility of perpetuating DNA damage. Therefore, we hypothesized that the application of high doses of minoxidil to the skin of hairless mice would increase the number of micronucleated erythrocytes (MNEs) in peripheral blood. The objective of this study was to evaluate the topical administration of high doses of minoxidil on peripheral blood erythrocytes of SKH1 mice by means of micronucleus assay. Minoxidil was administered on the entire body surface of mice every 12 or 24 h. Minoxidil dosing every 24 h increased the number of micronucleated polychromatic erythrocytes (MNPCEs), and dosing every 12 h increased the number of MNEs and MNPCEs, as compared to baseline and the negative control group. No decrease in polychromatic erythrocyte frequencies was observed in the minoxidil groups. Therefore, topical application of high minoxidil doses to mice can produce DNA damage, as observed through an increase in the number of MNEs, without producing cytotoxicity, possibly due to its mitogenic effect.

Blood heavy metals and DNA damage among children living in an informal E-waste processing area in Vietnam

2020 ◽  
pp. 1-19 ◽  
Author(s):  
Hien Thi Thu Ngo ◽  
Li Liang ◽  
Diep Bich Nguyen ◽  
Hai Ngoc Doan ◽  
Pensri Watchalayann
Keyword(s):  
Heavy Metals ◽  
Dna Damage ◽  
Waste Processing

scholarly journals Genotoxicity Study with Special Reference to Comet Test in the Blood Cells of Workers Exposed to Sewage Water

2014 ◽  
Vol 2014 ◽  
pp. 1-7
Author(s):  
Rajlaxmi Basu ◽  
Soumendra Nath Talapatra ◽  
Aniruddha Mukhopadhyay ◽  
Moumit Roy Goswami ◽  
Siddhartha Sankar Ray ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Dna Damage ◽  
Blood Cells ◽  
Adaptive Response ◽  
Suspended Solids ◽  
Total Suspended Solids ◽  
Total Dissolved Solids ◽  
Sewage Water ◽  
Total Iron ◽  
Aged Group

Awareness among sewage workers to occupational exposure is growing slowly in many developing countries. Lead (Pb) and cadmium (Cd) are present in sewage water and workers are exposed to these metals as a result of unprotected handling. These heavy metals exposures are responsible for DNA damage and lowering blood total iron (Fe) concentration. Zinc (Zn) is an element for promoting metallothionine expression and binds the free Cd. The total suspended solids (TSS), total dissolved solids (TDS), Pb, and Cd were estimated in sewage water. The whole blood Zn and Fe concentration and Pd and Cd were also estimated. Genotoxicity as indicated by DNA damage was studied by comet assay. It was observed that there were significant differences (P<0.001) of Pb and Cd concentration in blood for the sewage workers when compared with control population. DNA damage was also observed to be significantly (P<0.001) higher in the exposed groups but their blood Fe concentration was significantly lower, which may be the reason for their tendency for retention of blood Cd and make them more susceptible. This study also indicated that aged workers had higher blood Zn concentrations as compared to the younger (working < 20 years) workers. This may indicate a possible adaptive response. The present study proposes that younger (working < 20 years) group is more susceptible as compared to aged group (working > 20 years).

Blood Concentrations of Heavy Metals and DNA Damage Levels of Southeast Asia Female Immigrants

Epidemiology ◽  
2007 ◽  
Vol 18 (Suppl) ◽  
pp. S22
Author(s):  
W T Wu ◽  
T E Liu ◽  
S H Liou ◽  
T N Wu
Keyword(s):  
Heavy Metals ◽  
Dna Damage ◽  
Southeast Asia ◽  
Blood Concentrations ◽  
Female Immigrants
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