scholarly journals Recent Developments in SARS-CoV-2 Neutralizing Antibody Detection Methods

2021 ◽  
Author(s):  
Jo-Lewis Banga Ndzouboukou ◽  
Yan-di Zhang ◽  
Xiong-lin Fan
Keyword(s):  
Neutralizing Antibody ◽  
Detection Methods ◽  
Antibody Detection ◽  
Recent Developments

scholarly journals Recent Developments in Plasmonic Sensors of Phenol and Its Derivatives

2021 ◽  
Vol 11 (22) ◽  
pp. 10519
Author(s):  
Nguyễn Hoàng Ly ◽  
Sang Jun Son ◽  
Ho Hyun Kim ◽  
Sang-Woo Joo
Keyword(s):  
Phenolic Compounds ◽  
Optical Sensors ◽  
Noble Metals ◽  
Raw Material ◽  
Detection Methods ◽  
Localized Surface Plasmon ◽  
Trace Detection ◽  
Toxic Response ◽  
Recent Developments ◽  
Analytical Tools

Many scientists are increasingly interested in on-site detection methods of phenol and its derivatives because these substances have been universally used as a significant raw material in the industrial manufacturing of various chemicals of antimicrobials, anti-inflammatory drugs, antioxidants, and so on. The contamination of phenolic compounds in the natural environment is a toxic response that induces harsh impacts on plants, animals, and human health. This mini-review updates recent developments and trends of novel plasmonic resonance nanomaterials, which are assisted by various optical sensors, including colorimetric, fluorescence, localized surface plasmon resonance (LSPR), and plasmon-enhanced Raman spectroscopy. These advanced and powerful analytical tools exhibit potential application for ultrahigh sensitivity, selectivity, and rapid detection of phenol and its derivatives. In this report, we mainly emphasize the recent progress and novel trends in the optical sensors of phenolic compounds. The applications of Raman technologies based on pure noble metals, hybrid nanomaterials, and metal–organic frameworks (MOFs) are presented, in which the remaining establishments and challenges are discussed and summarized to inspire the future improvement of scientific optical sensors into easy-to-operate effective platforms for the rapid and trace detection of phenol and its derivatives.

scholarly journals DEVELOPMENT OF AN IMPROVED EPSTEIN-BARR VIRUS (EBV) NEUTRALIZING ANTIBODY ASSAY TO FACILITATE DEVELOPMENT OF A PROPHYLACTIC GP350-SUBUNIT EBV VACCINE

2020 ◽  
Vol 12 (1) ◽  
pp. e2020016
Author(s):  
Hui Liu ◽  
Lorraine Gemmell ◽  
Rui Lin ◽  
Fengrong Zuo ◽  
Henry H. Balfour ◽  
...  
Keyword(s):  
High Throughput ◽  
Neutralizing Antibody ◽  
Neutralization Assay ◽  
Epidemiologic Studies ◽  
Antibody Detection ◽  
Healthy Human ◽  
Barr Virus ◽  
Human Sera ◽  
Epstein Barr ◽  
Bioanalytical Assays

No licensed vaccine is available for prevention of EBV-associated diseases, and robust, sensitive, and high-throughput bioanalytical assays are needed to evaluate immunogenicity of gp350 subunit-based candidate EBV vaccines. Here we have developed and improved analytical tools for such a vaccine’s pre-clinical and clinical validation including a gp350-specific antibody detection assay and an EBV-GFP based neutralization assay for measuring EBV specific antibodies in human donors. The sensitivity of our previously published high-throughput EBV-GFP fluorescent focus (FFA)-based neutralization assay was further improved when guinea pig complement was supplemented using a panel of healthy human sera. Anti-gp350 antibody titers, which were evaluated using an anti-gp350 IgG ELISA assay optimized for capture and detection conditions, were moderately correlated to the FFA-based neutralization titers. Overall, these sensitive, and high-throughput bioanalytical assays are capable of characterizing the serologic response to natural EBV infection, with applications in evaluating EBV antibody status in epidemiologic studies and immunogenicity of candidate gp350-subunit EBV vaccines in clinical studies.

Diagnosis of schistosomiasis: antibody detection, with notes on parasitological and antigen detection methods

Parasitology ◽  
1999 ◽  
Vol 117 (7) ◽  
pp. 41-57 ◽  
Author(s):  
J. V. HAMILTON ◽  
M. KLINKERT ◽  
M. J. DOENHOFF
Keyword(s):  
Recombinant Dna ◽  
Infection Intensity ◽  
Public Health Problem ◽  
Antigen Detection ◽  
Detection Methods ◽  
Antibody Detection ◽  
Native Form ◽  
Keyhole Limpet Haemocyanin ◽  
Advantages And Disadvantages ◽  
Wide Range

Schistosomiasis remains a serious world-wide public health problem with a still unfulfilled need for routine cost-effective methods of diagnosis. Such methods are required not only for people in endemic areas, but increasingly for tourists who may have become infected during visits to such places. This article reviews the wide range of immunoassays and antigenic preparations that have been shown to have potential for diagnosis of schistosomiasis by the indirect method of antibody detection. Antigens in native form derived from cercariae, adult worms and eggs are considered, as well as schistosome antigens produced by recombinant DNA technology and the schistosome cross-reactive antigen, keyhole limpet haemocyanin (KLH). Respective advantages and disadvantages of antibody detection, circulating antigen detection and parasitological methods of diagnosis are analysed. It is suggested that due to the relative insensitivity of both parasitology and antigen detection, antibody detection methods could find increasing use in situations of low infection intensity.

DNA methylation detection: recent developments in bisulfite free electrochemical and optical approaches

The Analyst ◽  
2018 ◽  
Vol 143 (20) ◽  
pp. 4802-4818 ◽  
Author(s):  
Ripon Bhattacharjee ◽  
Sofia Moriam ◽  
Muhammad Umer ◽  
Nam-Trung Nguyen ◽  
Muhammad J. A. Shiddiky
Keyword(s):  
Dna Methylation ◽  
Detection Methods ◽  
Electrochemical Biosensors ◽  
Bisulfite Treatment ◽  
Free Dna ◽  
Recent Developments

The recent development of optical and electrochemical biosensors for bisulfite treatment free DNA methylation detection methods have been reviewed. The major challenges associated with the bisulfite treatment in DNA methylation detection and their potential solutions are also discussed.

Antiglycolipid antibodies in Guillain-Barré and Fisher syndromes: discovery, current status and future perspective

2020 ◽  
pp. jnnp-2020-325053
Author(s):  
Susumu Kusunoki ◽  
Hugh J Willison ◽  
Bart C Jacobs
Keyword(s):  
Acute Phase ◽  
Axonal Neuropathy ◽  
Detection Methods ◽  
Antibody Detection ◽  
Current Status ◽  
Future Perspective ◽  
Acute Motor Axonal Neuropathy ◽  
Cns Diseases ◽  
Fisher Syndrome ◽  
Guillain Barré

Guillain-Barré syndrome (GBS) and Fisher syndrome (FS) are acute autoimmune neuropathies, often preceded by an infection. Antiglycolipid antibody titres are frequently elevated in sera from the acute-phase patients. Particularly, IgG anti-GQ1b antibodies are positive in as high as 90% of FS cases and thus useful for diagnosis. The development of animal models of antiglycolipid antibody-mediated neuropathies proved that some of these antibodies are directly involved in the pathogenetic mechanisms by binding to the regions where the respective target glycolipid is specifically localised. Discovery of the presence of the antibodies that specifically recognise a new conformational epitope formed by two different gangliosides (ganglioside complex) in the acute-phase sera of some patients with GBS suggested the carbohydrate–carbohydrate interaction between glycolipids. This finding indicated the need for further research in basic glycobiological science. Antiglycolipid antibodies, in particular antigangliosides antibodies, are mostly detected in acute motor axonal neuropathy type of GBS and in FS, and less frequently in the acute inflammatory demyelinating polyneuropathy (AIDP) type of GBS or in central nervous system (CNS) diseases. In the future, the search for the putative antibodies in AIDP and those that might be present in CNS diseases should continue. In addition, more efficient standardisation of antiglycolipid antibody detection methods and use as biomarkers in daily clinical practice in neurology is needed.

scholarly journals Recent Developments of Electrochemical and Optical Biosensors for Antibody Detection

2019 ◽  
Vol 21 (1) ◽  
pp. 134 ◽  
Author(s):  
Wei Xu ◽  
Daniel Wang ◽  
Derek Li ◽  
Chung Chiun Liu
Keyword(s):  
Point Of Care ◽  
Low Cost ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Disease Diagnosis ◽  
Antibody Detection ◽  
Recent Developments ◽  
Care Systems ◽  
Point Of Care Systems ◽  
Detection Of Biomarkers

Detection of biomarkers has raised much interest recently due to the need for disease diagnosis and personalized medicine in future point-of-care systems. Among various biomarkers, antibodies are an important type of detection target due to their potential for indicating disease progression stage and the efficiency of therapeutic antibody drug treatment. In this review, electrochemical and optical detection of antibodies are discussed. Specifically, creating a non-label and reagent-free sensing platform and construction of an anti-fouling electrochemical surface for electrochemical detection are suggested. For optical transduction, a rapid and programmable platform for antibody detection using a DNA-based beacon is suggested as well as the use of bioluminescence resonance energy transfer (BRET) switch for low cost antibody detection. These sensing strategies have demonstrated their potential for resolving current challenges in antibody detection such as high selectivity, low operation cost, simple detection procedures, rapid detection, and low-fouling detection. This review provides a general update for recent developments in antibody detection strategies and potential solutions for future clinical point-of-care systems.

52-P: Correlations of donor-specific antibody detection methods

2009 ◽  
Vol 70 ◽  
pp. S37
Author(s):  
Younhee Park ◽  
Hyon-Suk Kim ◽  
Dong Il Won ◽  
Hae Jin Kim ◽  
Yu Seun Kim
Keyword(s):  
Specific Antibody ◽  
Detection Methods ◽  
Antibody Detection
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