Infliximab Increases the Tissue Contents of Type-I and Type-III Collagen in Colorectal Segments Without Fecal Stream After Hartmann’s Procedure

Fibers composed of different types of collagen cannot be differentiated by conventional electron microscopic stains. We are developing staining procedures aimed at identifying collagen fibers of different types.Pt(Gly-L-Met)Cl binds specifically to sulfur-containing amino acids. Different collagens have methionine (met) residues at somewhat different positions. A good correspondence has been reported between known met positions and Pt(GLM) bands in rat Type I SLS (collagen aggregates in which molecules lie adjacent to each other in exact register). We have confirmed this relationship in Type III collagen SLS (Fig. 1).

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Subpopulations of rat lung fibroblasts with different amounts of type I and type III collagen mRNAs.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)39323-8 ◽

1990 ◽

Vol 265 (11) ◽

pp. 6286-6290

Author(s):

E Breen ◽

V M Falco ◽

M Absher ◽

K R Cutroneo

Keyword(s):

Lung Fibroblasts ◽

Type Iii Collagen ◽

Type I ◽

Rat Lung ◽

Type Iii

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Change in phenotype in long-term cultures of a clonal rat bone cell line: switch to the synthesis of α1 (I)-trimer collagen

Canadian Journal of Biochemistry and Cell Biology ◽

10.1139/o84-063 ◽

1984 ◽

Vol 62 (6) ◽

pp. 462-469 ◽

Cited By ~ 2

Author(s):

Hardy Limeback ◽

Kichibee Otsuka ◽

Kam-Ling Yao ◽

Jane E. Aubin ◽

Jaro Sodek

Keyword(s):

Collagen Synthesis ◽

Type I Collagen ◽

Bone Cell ◽

Detectable Effect ◽

Prostaglandin E ◽

Intracellular Camp ◽

Type Iii Collagen ◽

Type I ◽

Type Iii ◽

Type V

A number of bone cell clones isolated from rat calvaria have been maintained in culture for more than 3 years. Several of these clones have undergone dramatic changes in phenotype. One of these clones, RGB 2.2, was observed originally to have a fibroblastic morphology in culture and to respond to parathyroid hormone (PTH), but not prostaglandin E2 (PGE2), with an increase in intracellular cAMP. Throughout several passages in early subcultures, these cells synthesized mostly type I collagen, with small amounts of type III and type V collagens. Whereas PTH had no detectable effect on collagen synthesis, PGE2 decreased the amount of total cell layer collagen, with the greatest effect on type III collagen, while increasing the proportion of type V collagen. Subsequent studies on these cells during 3 years in culture have indicated changes in their phenotype including a progressive change in morphology to a more cuboidal shape and a change in collagen synthesis, the cells producing large amounts of the "embryonic" collagen, α1(I) trimer. The reason(s) for the change in collagen expression is unknown, but may be the result of a change in which gene(s) is being expressed.

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PERITONEAL ADHESIONS TYPE I, III AND TOTAL COLLAGEN ON POLYPROPYLENE AND COATED POLYPROPYLENE MESHES: EXPERIMENTAL STUDY IN RATS

ABCD Arquivos Brasileiros de Cirurgia Digestiva (São Paulo) ◽

10.1590/0102-6720201700020001 ◽

2017 ◽

Vol 30 (2) ◽

pp. 77-82 ◽

Cited By ~ 2

Author(s):

Lucas Félix ROSSI ◽

Manoel Roberto Maciel TRINDADE ◽

Armando José D`ACAMPORA ◽

Luise MEURER

Keyword(s):

Type I Collagen ◽

Cell Types ◽

Type Iii Collagen ◽

Type I ◽

Abdominal Adhesions ◽

Peritoneal Adhesions ◽

Type Iii ◽

Total Collagen

ABSTRACT Background: Hernia correction is a routinely performed treatment in surgical practice. The improvement of the operative technique and available materials certainly has been a great benefit to the quality of surgical results. The insertion of prostheses for hernia correction is well-founded in the literature, and has become the standard of treatment when this type of disease is discussed. Aim: To evaluate two available prostheses: the polypropylene and polypropylene coated ones in an experimental model. Methods: Seven prostheses of each kind were inserted into Wistar rats (Ratus norvegicus albinus) in the anterior abdominal wall of the animal in direct contact with the viscera. After 90 days follow-up were analyzed the intra-abdominal adhesions, and also performed immunohistochemical evaluation and videomorphometry of the total, type I and type III collagen. Histological analysis was also performed with hematoxylin-eosin to evaluate cell types present in each mesh. Results: At 90 days the adhesions were not different among the groups (p=0.335). Total collagen likewise was not statistically different (p=0.810). Statistically there was more type III collagen in the coated polypropylene group (p=0.039) while type I was not different among the prostheses (p=0.050). The lymphocytes were statistically more present in the polypropylene group (p=0.041). Conclusion: The coated prosthesis was not different from the polypropylene one regarding the adhesion. Total and type I collagen were not different among the groups, while type III collagen was more present on the coated mesh. There was a greater number of lymphocytes on the polypropylene mesh.

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A matrix metalloproteinase-generated neoepitope of CRP can identify knee and multi-joint inflammation in osteoarthritis

Arthritis Research & Therapy ◽

10.1186/s13075-021-02610-y ◽

2021 ◽

Vol 23 (1) ◽

Author(s):

Louie C. Alexander ◽

Grant McHorse ◽

Janet L. Huebner ◽

Anne-Christine Bay-Jensen ◽

Morten A. Karsdal ◽

...

Keyword(s):

Synovial Fluid ◽

Matrix Metalloproteinase ◽

Type I Collagen ◽

Joint Inflammation ◽

Cartilage Degradation ◽

Womac Pain ◽

Type Iii Collagen ◽

Type I ◽

Radiographic Imaging ◽

Type Iii

Abstract Objective To compare C-reactive protein (CRP) and matrix metalloproteinase-generated neoepitope of CRP (CRPM) as biomarkers of inflammation and radiographic severity in patients with knee osteoarthritis. Methods Participants with symptomatic osteoarthritis (n=25) of at least one knee underwent knee radiographic imaging and radionuclide etarfolatide imaging to quantify inflammation of the knees and other appendicular joints. For purposes of statistical analysis, semi-quantitative etarfolatide and radiographic imaging scores were summed across the knees; etarfolatide scores were also summed across all joints to provide a multi-joint synovitis measure. Multiple inflammation and collagen-related biomarkers were measured by ELISA including CRP, CRPM, MMP-generated neoepitopes of type I collagen and type III collagen in serum (n=25), and CD163 in serum (n=25) and synovial fluid (n=18). Results BMI was associated with CRP (p=0.001), but not CRPM (p=0.753). Adjusting for BMI, CRP was associated with radiographic knee osteophyte score (p=0.002), while CRPM was associated with synovitis of the knee (p=0.017), synovitis of multiple joints (p=0.008), and macrophage marker CD163 in serum (p=0.009) and synovial fluid (p=0.03). CRP correlated with MMP-generated neoepitope of type I collagen in serum (p=0.045), and CRPM correlated with MMP-generated neoepitope of type III collagen in serum (p<0.0001). No biomarkers correlated with age, knee pain, or WOMAC pain. Conclusions To our knowledge, this is the first time that CRPM has been shown to be associated with knee and multi-joint inflammation based on objective imaging (etarfolatide) and biomarker (CD163) measures. These results demonstrate the capability of biomarker measurements to reflect complex biological processes and for neoepitope markers to more distinctly reflect acute processes than their precursor proteins. CRPM is a promising biomarker of local and systemic inflammation in knee OA that is associated with cartilage degradation and is independent of BMI. CRPM is a potential molecular biomarker alternative to etarfolatide imaging for quantitative assessment of joint inflammation.

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Asiaticoside induces cell proliferation and collagen synthesis in human dermal fibroblasts

Zinc supplementation improves heme biosynthesis in rats exposed to lead ◽

10.18051/univmed.2015.v34.96-103 ◽

2015 ◽

Vol 34 (2) ◽

pp. 96

Author(s):

Linda Yuliati ◽

Etik Mardliyati ◽

Kusmarinah Bramono ◽

Hans Joachim Freisleben

Keyword(s):

Cell Proliferation ◽

Wound Healing ◽

Retinoic Acid ◽

Collagen Synthesis ◽

Active Agent ◽

Dermal Fibroblasts ◽

Type Iii Collagen ◽

Type I ◽

Human Dermal Fibroblasts ◽

Type Iii

Background Asiatiocoside, a saponin component isolated from Centella asiatica can improve wound healing by promoting the proliferation of human dermal fibroblasts (HDF) and synthesis of collagen. The skin-renewing cells and type I and III collagen synthesis decrease with aging, resulting in the reduction of skin elasticity and delayed wound healing. Usage of natural active compounds from plants in wound healing should be evaluated and compared to retinoic acid as an active agent that regulates wound healing. The aim of this study was to compare and evaluate the effect of asiaticoside and retinoic acid to induce greater cell proliferation and type I and III collagen synthesis in human dermal fibroblast. Methods Laboratory experiments were conducted using human dermal fibroblasts (HDF) isolated from human foreskin explants. Seven passages of HDF were treated with asiaticoside and retinoic acid at several doses and incubated for 24 and 48 hours. Cell viability in all groups was tested with the MTT assay to assess HDF proliferation. Type I and III collagen synthesis was examined using the respective ELISA kits. Analysis of variance was performed to compare the treatment groups. Results Asiaticoside had significantly stronger effects on HDF proliferation than retinoic acid (p<0.05). The type III collagen production was significantly greater induction with asiaticoside compared to retinoic acid (p<0.05). Conclusion Asiaticoside induces HDF proliferation and type I and III collagen synthesis in a time- and dose-dependent pattern. Asiaticoside has a similar effect as retinoic acid on type I and type III collagen synthesis.

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Metabolism of rabbit skin collagen. Differences in the apparent turnover rates of type-I- and type-III-collagen precursors determined by constant intravenous infusion of labelled amino acids

Biochemical Journal ◽

10.1042/bj1810075 ◽

1979 ◽

Vol 181 (1) ◽

pp. 75-82 ◽

Cited By ~ 23

Author(s):

S P Robins

Keyword(s):

Amino Acid ◽

Collagen Synthesis ◽

Specific Radioactivity ◽

Deae Cellulose ◽

Type Iii Collagen ◽

Type I ◽

Turnover Rates ◽

Type Iii ◽

Type I Procollagen ◽

Skin Collagen

Growing rabbits were infused for up to 10 h with labelled proline, tyrosine and leucine to achieve plateau conditions within body free pools, for [3H]proline infusion, blood free-proline specific radioactivity remained constant after about 1 h. For individual animals, type-I- and type-III-collagen precursors were isolated by precipitation with (NH4)2SO4 and DEAE-cellulose chromatography. Experiments where 3H- and 14C-labelled proline and tyrosine were infused concurrently for different periods of time showed that type I procollagen reached plateau specific radioactivity within 3 h and 90% of the plateau value after 2 h infusion, corresponding to a calculated apparent t 1/2 of less than 26 min. Plateau values for type I procollagen were taken as precursor amino acid pool specific radioactivities. The type-III-collagen-precursor fractions consistently showed lower rates of label incorporation and, by assuming that both type I and type III collagens are synthesized from the same amino acid pools, kinetic analysis revealed an apparent t 1/2 for the isolated type-III-collagen precursors of 3.9 h. For proline, there were large variations between animals in the ratio between the precursor pool for collagen synthesis and the skin homogenate free pool (0.31 +/- 0.13, mean +/- S.D.), so that collagen-synthesis rates based solely on total tissue free-pool values for proline are subject to large and inconsistent errors.

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Type VI collagen induces cardiac myofibroblast differentiation: implications for postinfarction remodeling

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.00321.2005 ◽

2006 ◽

Vol 290 (1) ◽

pp. H323-H330 ◽

Cited By ~ 103

Author(s):

Jennifer E. Naugle ◽

Erik R. Olson ◽

Xiaojin Zhang ◽

Sharon E. Mase ◽

Charles F. Pilati ◽

...

Keyword(s):

Cardiac Fibrosis ◽

Type I Collagen ◽

In Vivo Model ◽

Type Iii Collagen ◽

Type I ◽

Myofibroblast Differentiation ◽

Type Vi Collagen ◽

Collagen Substrate ◽

Type Iii

Cardiac fibroblast (CF) proliferation and differentiation into hypersecretory myofibroblasts can lead to excessive extracellular matrix (ECM) production and cardiac fibrosis. In turn, the ECM produced can potentially activate CFs via distinct feedback mechanisms. To assess how specific ECM components influence CF activation, isolated CFs were plated on specific collagen substrates (type I, III, and VI collagens) before functional assays were carried out. The type VI collagen substrate potently induced myofibroblast differentiation but had little effect on CF proliferation. Conversely, the type I and III collagen substrates did not affect differentiation but caused significant induction of proliferation (type I, 240.7 ± 10.3%, and type III, 271.7 ± 21.8% of basal). Type I collagen activated ERK1/2, whereas type III collagen did not. Treatment of CFs with angiotensin II, a potent mitogen of CFs, enhanced the growth observed on types I and III collagen but not on the type VI collagen substrate. Using an in vivo model of myocardial infarction (MI), we measured changes in type VI collagen expression and myofibroblast differentiation after post-MI remodeling. Concurrent elevations in type VI collagen and myofibroblast content were evident in the infarcted myocardium 20-wk post-MI. Overall, types I and III collagen stimulate CF proliferation, whereas type VI collagen plays a potentially novel role in cardiac remodeling through facilitation of myofibroblast differentiation.

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Abstract 508: Prolonged Thrombus Resolution Leading to Abnormal Collagen Fibrillogenesis and Angiogenesis in Injured Arteries of Type III Collagen-Deficient Mice: a Paradoxical Mechanism for ‘Tissue Fragility’ in Vascular Ehlers-Danlos Syndrome and Spontaneous Cervical Artery Dissection

Arteriosclerosis Thrombosis and Vascular Biology ◽

10.1161/atvb.33.suppl_1.a508 ◽

2013 ◽

Vol 33 (suppl_1) ◽

Author(s):

Amy J Reid ◽

Yuqiang Bai ◽

Lorenzo F Perez ◽

Naomi Ogawa ◽

L. M Buja ◽

...

Keyword(s):

3D Culture ◽

Vascular Complications ◽

Artery Dissection ◽

Cervical Artery Dissection ◽

Type Iii Collagen ◽

Type I ◽

Ehlers Danlos Syndrome ◽

Type Iii ◽

Inflammatory Phase ◽

Vascular Channels

Patients with COL3A1 mutations that impede secretion of type III collagen into matrix develop wounds that heal poorly following cutaneous injury. They are also predisposed to vascular complications like catastrophic rupture or dissection of cervical arteries and stroke. We sought to determine if low production of type III collagen would also interrupt arterial thrombus resolution, a process resembling wound healing, and whether defects contribute to risk of dissection. We injured cervical elastic arteries in mice by ligation of the left common carotid, halting proximal blood flow. Strikingly, injured arteries from Col3a1 +/- mice develop thrombi over three weeks that resist resolution more often than those in wild-type littermates ( p =.002). The unresolved thrombi in Col3a1 +/- arteries also retain a higher burden of macrophages ( p =.043) and proliferative α-actin-positive cells ( p =.034), while mutant arteries display vascular channels within the media ( p =.015) that are partially lined with endothelial cells, consistent with residual neoangiogenesis. At two weeks, burdens of macrophages ( p =.009), proliferative cells ( p =.028), and vascular channels ( p =.019) are also higher in Col3a1 +/- arteries, despite actively resolving thrombi of equal length between the two groups ( p =.338), suggesting that neoangiogenesis results specifically from lower Col3a1 dose. Treating injured mice with rapamycin halts thrombus resolution in the early inflammatory phase of both genotypes and normalizes the incidence of vascular channels in Col3a1 +/- arteries at three weeks ( p =1.00), suggesting that vascular channels result secondary to thrombus resolution and not from structural weakness in type III collagen deficient arteries. Patient-derived COL3A1 mutant myofibroblasts in 3D culture models of fibrin remodeling accumulate less extracellular type I collagen following TGFβ1 stimulation but persistently upregulate expression of Acta2 , relative to control cells whose Acta2 expression levels peak and subside as collagen accumulates by seven days. These data implicate dysregulated thrombus remodeling in response to injury that increases the risk for medial neoangiogenesis, which may also increase the risk for dissection in affected arteries after injury.

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Effects of Fractional CO2 Laser Treatment on Subglottic Scar in a Rabbit Model

Otolaryngology ◽

10.1177/0194599820978256 ◽

2020 ◽

pp. 019459982097825

Author(s):

Kastley Marvin ◽

Isaac Schwartz ◽

Edward Utz ◽

Justin Wilson ◽

Christopher Johnson ◽

...

Keyword(s):

Laser Treatment ◽

Type I Collagen ◽

Medical Center ◽

Academic Medical Center ◽

Rabbit Model ◽

Study Endpoint ◽

Type Iii Collagen ◽

Type I ◽

Respiratory Complications ◽

Type Iii

Objective The objective of this study was to investigate the effects of fractional CO2 laser on subglottic scar. Study Design Randomized controlled animal study. Setting Academic medical center. Methods Subglottic scar was induced in 12 New Zealand white rabbits via an endoscopic brush technique. This was followed by an open airway surgery that included vertical division of the cricoid and proximal trachea. Eight rabbits underwent fractional CO2 laser treatment of the scar via a Lumenis Ultrapulse Deep FX handpiece. Four rabbits underwent the open surgical approach without laser treatment. Bronchoscopy was performed at weeks 1, 2, 4, and 8. The animals were euthanized and laryngotracheal complexes harvested 12 weeks postsurgery. Immunohistochemistry was performed to determine the collagen composition of treated and untreated scars. Results All 12 subjects survived to the study endpoint with no significant respiratory complications, despite 10 of 12 developing some degree of lateral tracheal narrowing. The median ratio of type I collagen to type III collagen in the laser group (1.57) was significantly more favorable than that of the untreated group (2.84; P = .03). Conclusion Treatment with fractional CO2 laser appears to have similar effects on subglottic scars as with cutaneous scars, improving the ratio of type I to type III collagen. Additionally, we developed an open airway approach in the rabbit model to deliver fractional CO2 laser treatment to the subglottis without introducing respiratory complications or compromising survival.

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