A cryopreservation protocol for ex situ conservation of terrestrial orchids using asymbiotic primary and secondary (adventitious) protocorms

2015 ◽  
Vol 52 (2) ◽  
pp. 185-195 ◽  
Author(s):  
Betty Mauliya Bustam ◽  
Kingsley Dixon ◽  
Eric Bunn
Keyword(s):  
Ex Situ Conservation ◽  
Ex Situ ◽  
Terrestrial Orchids ◽  
Cryopreservation Protocol

Development of sperm cryopreservation protocol of endangered spiny eel, Mastacembelus armatus (Lacepede 1800) for ex-situ conservation

Cryobiology ◽  
2016 ◽  
Vol 73 (3) ◽  
pp. 316-323 ◽  
Author(s):  
M. Mahfujur Rahman ◽  
M. Rahmat Ali ◽  
M. Rafiqul Islam Sarder ◽  
M. Fazlul Awal Mollah ◽  
Najmus Sakib Khan
Keyword(s):  
Ex Situ Conservation ◽  
Ex Situ ◽  
Sperm Cryopreservation ◽  
Mastacembelus Armatus ◽  
Cryopreservation Protocol

scholarly journals Orchid exploration in Mount Bintan Besar Protected Forest, Bintan Island, Riau Islands Province, Sumatra, Indonesia

2018 ◽  
Vol 19 (3) ◽  
pp. 1081-1088 ◽  
Author(s):  
DWI MURTI PUSPITANINGTYAS
Keyword(s):  
Random Sampling ◽  
Ex Situ Conservation ◽  
New Guinea ◽  
Ex Situ ◽  
Terrestrial Orchids ◽  
On This Island ◽  
Plant Collection

Puspitaningtyas DM. 2018. Orchid exploration in Mount Bintan Besar Protected Forest, Bintan Island, Riau IslandsProvince, Sumatra, Indonesia. Biodiversitas 19: 1081-1088. Sumatra is one of the main islands in Indonesia. The biodiversity is higherthan Java, but still below compare to Borneo and New Guinea. About 1,118 species of orchids are found growing in Sumatra, which41% of these are endemic to Sumatra. The exploration activities were conducted at Gunung Bintan Besar Protected Forest, Bintanisland-Sumatra. Orchid exploration was conducted in this area to collect living plants for ex situ conservation purpose. Orchid inventoryto record orchid diversity in this area, based on plant collection by purposive random sampling. The results of the study recordedapproximately 23 orchid collection numbers found in that area. These were representative of 15 genera and consist of 14 species ofepiphyte orchids and 9 species of terrestrial orchids. Most orchids found in this area are lowland orchid which are very common inSumatra. Dendrobium metachilinum Rchb.f., Dendrobium leonis (Lindl.) Rchb.f. and Plocoglottis lowii Rchb.f. are unique orchidsspecies found on this island.

scholarly journals Ultra-low temperature conservation of Brazilian Pine embryogenic cultures

2014 ◽  
Vol 86 (4) ◽  
pp. 2057-2064 ◽  
Author(s):  
GRASIELA DEMARCHI ◽  
VALDIR M. STEFENON ◽  
NEUSA STEINER ◽  
FELIPE N. VIEIRA ◽  
LIRIO L. DAL VESCO ◽  
...  
Keyword(s):  
Low Temperature ◽  
Cell Viability ◽  
Biochemical Composition ◽  
Ex Situ Conservation ◽  
Ex Situ ◽  
Embryogenic Cultures ◽  
Morphological Alterations ◽  
New Horizons ◽  
Immature Seeds ◽  
Cryopreservation Protocol

This study aimed to establish a cryopreservation protocol for embryogenic cultures of A. angustifolia, enabling the ex situ conservation of the species. Embryogenic cultures were established from immature seeds and treated with variations of the cryoprotectant solutions SuDG, SoD and PVS2 prior to immersion in liquid nitrogen. Cell viability was evaluated after 30, 60 and 90 days of re-growth. The highest re-growth without morphological alterations and with normal biochemical composition was obtained with the PVS2 solution with 40 min immersion in ethanol (-20 °C). This procedure opens new horizons for the ex situ conservation of the species genetic.

scholarly journals Orchids Diversity in the Sicikeh-Cikeh Forest, North Sumatra, Indonesia

2019 ◽  
Vol 20 (4) ◽  
pp. 1087-1096 ◽  
Author(s):  
SRI HARTINI
Keyword(s):  
Plant Species ◽  
Random Sampling ◽  
Ex Situ Conservation ◽  
Ex Situ ◽  
Mountain Forest ◽  
Epiphytic Orchids ◽  
Protection Forest ◽  
Terrestrial Orchids ◽  
Plant Collection ◽  
North Sumatra

Abstract. Hartini S. 2019. Orchids Diversity in the Sicikeh-Cikeh Forest, North Sumatra, Indonesia. Biodiversitas 20: 1087-1096. Sicikeh-cikeh forest includes three forest areas, namely Adian Tinjoan Customary Forest, Adian Tinjoan Protection Forest, and Taman Wisata Alam Sicikeh-cikeh. Typical vegetation of this area is mountain forest with large diversity of plant species. Among the species, orchid is one of the potential plants found diversely in this location and has not yet been recorded. The exploration activities were conducted at Sicikeh-cikeh forest. Orchid exploration was conducted to collect living plants for ex situ conservation purpose. Orchid inventory, to record orchid diversity in this area, was based on plant collection by purposive random sampling. The results of the study recorded approximately 102 different species from 30 genera of orchids in this area. Typical epiphytic and terrestrial highland orchids were found and very common in Sumatra. Among the species found are endemic Sumatra, such as Coelogyne brachygyne, Coelogyne salmonicolor, Dendrobium kruiense, Epigeneium pulchellum, Thrixspermum gombakense, Corybas stenotribonos and Paphiopedilum tonsum. Interesting terrestrial orchids include Kuhlhasseltia javanica, Neuwiedia zollingeri var. javanica, Paphiopedilum tonsum, Phaius callosus, Corybas stenotribonos, Calanthe aurantiaca, Calanthe chrysoglossoides, and Calanthe pulchra. While interesting epiphytic orchids include Bulbophyllum absconditum, Bulbophyllum coloriferum, Coelogyne brachygyne, Epigenium pulchellum, Eria bractescens, Eria pachystachya, Pholidota gibbosa, and Thrixspermum amplexicaule.

scholarly journals Cryopreservation of spermatozoa of indigenous stinging catfish, Heteropneustes fossilis (Bloch) for ex-situ conservation

2020 ◽  
Vol 32 (1) ◽  
pp. 11-22
Author(s):  
MD. RAFIQUL ISLAM SARDER ◽  
MD. ABUL KALAM AZAD ◽  
K.M. SHAHRIAR NAZRUL ◽  
MOHAMMAD RASHED
Keyword(s):  
Ex Situ Conservation ◽  
Egg Yolk ◽  
Ringer Solution ◽  
The Other ◽  
Ex Situ ◽  
Nacl Solution ◽  
Heteropneustes Fossilis ◽  
Significant Difference ◽  
Cryopreserved Sperm ◽  
Cryopreservation Protocol

An experiment was conducted to develop cryopreservation protocol for spermatozoa of stinging catfish, Heteropneustes fossilis and to use the cryopreserved sperm in its breeding trials. The activation of sperm motility at various concentrations of NaCl solution was tested and complete activation and inhibition of sperm were obtained at 0.4% and 0.9% to 1% NaCl solution respectively. In toxicity test, sperms were incubated with DMSO, methanol and ethanol at 5, 10, and 15% concentrations where DMSO and methanol produced better motility at 5 and 10% concentration with Alsever’s solution and egg-yolk citrate at 5 and- 10 min incubation period. Three extenders- Alsever’s solution, egg-yolk citrate and Ginsburg Fish Ringer solution and three cryoprotectants- DMSO, methanol and ethanol were used for cryopreservation of sperm, and among the diluents, Alsever’s solution with 10% DMSO showed best performance producing 77.50±3.22% post-thaw motility. On the other hand, egg-yolk citrate and Ginsburg Fish Ringer solution along with 10% DMSO produced 63.75±2.39% and 62.50±3.22% post-thaw motility, respectively. Sperm preserved with Alsever’s solution plus 10% DMSO produced 52.5±3.34% and 38.0±2.39%fertilization and hatching, and those preserved with Alsever’s solution plus 10% methanol produced 46.9±3.11% and 32.7±2.70% fertilization and hatching respectively. The fry produced using cryopreserved and fresh sperm grew well and no significant difference (p>0.05) was found between two groups.

Simultaneous encapsulation of seed and mycorrhizal fungi for long-term storage and propagation of terrestrial orchids

2008 ◽  
Vol 56 (7) ◽  
pp. 609 ◽  
Author(s):  
Karen D. Sommerville ◽  
John P. Siemon ◽  
Chris B. Wood ◽  
Catherine A. Offord
Keyword(s):  
Mycorrhizal Fungi ◽  
Ex Situ Conservation ◽  
Alginate Beads ◽  
Ex Situ ◽  
Storage Duration ◽  
Long Term Storage ◽  
Mycorrhizal Associations ◽  
Terrestrial Orchids

Ex situ conservation of threatened terrestrial orchids requires the simultaneous conservation of their mycorrhizal associations. A method for encapsulating both seed and fungi in alginate beads (known as encapsulation–dehydration) was applied to the storage and propagation of two endangered orchid species in NSW, Australia—Pterostylis saxicola D.L.Jones & M.A.Clem. and Diuris arenaria D.L.Jones. We tested the effect of storage duration and temperature on fungal recovery and germination potential in vitro, and recorded survival for seedlings subsequently transferred to potting mix. Storage at 23°C significantly reduced fungal recovery and germination for both species after only 3 months (P < 0.05), whereas storage at 4°C significantly reduced fungal recovery for P. saxicola after 6 months (P < 0.05). Storage for 6 months at −18 and −196°C had no significant effect on the fungal recovery and germination percentages of either species. All beads transferred directly from in vitro culture to potting mix resulted in the establishment of at least one seedling, and production of a healthy tuberoid, when transferred near the commencement of the natural growing season. The encapsulation–dehydration method may have a practical application for use in ex situ conservation of other terrestrial orchids, as well as their mycorrhizal fungi.

Ex-situ conservation of medicinal plant genetic resources managed by national agrobiodiversity center of Korea

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
JS Sung ◽  
CW Jeong ◽  
YY Lee ◽  
HS Lee ◽  
YA Jeon ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Genetic Resources ◽  
Ex Situ Conservation ◽  
Plant Genetic Resources ◽  
Ex Situ

The Nagoya Protocol and the Role of Research Resource Centers as Ex-situ Conservation Institutes

2018 ◽  
Vol 26 (2) ◽  
pp. 47-69
Author(s):  
Hyeyeon Im ◽  
Minkyung Jung ◽  
Kyungsook Ahn ◽  
Ki Hyun Ryu
Keyword(s):  
Ex Situ Conservation ◽  
Ex Situ ◽  
Nagoya Protocol

Ex Situ Conservation of Rare Medicinal Plants in the Botanical Garden (Institute) of Academy of Sciences of Moldova

2013 ◽  
Vol 40 (1) ◽  
pp. 43-48
Author(s):  
Nina Ciocârlan
Keyword(s):  
Native Species ◽  
Ex Situ Conservation ◽  
Botanical Garden ◽  
Ex Situ ◽  
Aromatic Plants ◽  
Medicinal And Aromatic Plants ◽  
Field Collection ◽  
Ecological Flexibility ◽  
Cultivation Techniques ◽  
Academy Of Sciences

Abstract This work refers to the native species of genus Astragalus L. (A. dasyanthus, A. ponticus), Adonis L. (A. vernalis, A. wolgensis) and Digitalis L. (D. lanata, D. grandiflora). The plants are cultivated in the Botanical Garden of Moldova in the field collection of the medicinal and aromatic plants. Investigation includes propagation aspects, research into cultivation techniques and conservation measures. The biological particularities and the phenologic rhythm are also registered. The obtained data shows the ecological flexibility of species and the possibility of preserving them in culture.

SEED FEATURES OF IMPORTANT TIMBER SPECIES FROM THE FLOODPLAIN VÁRZEA FOREST: IMPLICATIONS FOR EX SITU CONSERVATION PROGRAMS IN THE AMAZON

2013 ◽  
Vol 9 (2) ◽  
pp. 7-19 ◽  
Author(s):  
Auristela Dos Santos Conserva ◽  
Denise Garcia de Santana ◽  
Maria Teresa Fernandez Piedade
Keyword(s):  
Ex Situ Conservation ◽  
Ex Situ ◽  
Timber Species ◽  
Conservation Programs
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