Ultra-low temperature conservation of Brazilian Pine embryogenic cultures

This study aimed to establish a cryopreservation protocol for embryogenic cultures of A. angustifolia, enabling the ex situ conservation of the species. Embryogenic cultures were established from immature seeds and treated with variations of the cryoprotectant solutions SuDG, SoD and PVS2 prior to immersion in liquid nitrogen. Cell viability was evaluated after 30, 60 and 90 days of re-growth. The highest re-growth without morphological alterations and with normal biochemical composition was obtained with the PVS2 solution with 40 min immersion in ethanol (-20 °C). This procedure opens new horizons for the ex situ conservation of the species genetic.

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A cryopreservation protocol for ex situ conservation of terrestrial orchids using asymbiotic primary and secondary (adventitious) protocorms

In Vitro Cellular & Developmental Biology - Plant ◽

10.1007/s11627-015-9732-7 ◽

2015 ◽

Vol 52 (2) ◽

pp. 185-195 ◽

Cited By ~ 4

Author(s):

Betty Mauliya Bustam ◽

Kingsley Dixon ◽

Eric Bunn

Keyword(s):

Ex Situ Conservation ◽

Ex Situ ◽

Terrestrial Orchids ◽

Cryopreservation Protocol

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Designing ex-situ conservation strategies for seeds storage of Piper aduncum and P. hispidinervum through cryopreservation and low-temperature techniques

Journal of Forest Research ◽

10.1080/13416979.2017.1381494 ◽

2017 ◽

Vol 22 (6) ◽

pp. 380-385

Author(s):

Tatiane Loureiro Da Silva ◽

Hugo Teixeira Gomes ◽

Jonny Everson Scherwinski-Pereira

Keyword(s):

Low Temperature ◽

Ex Situ Conservation ◽

Ex Situ ◽

Conservation Strategies ◽

Piper Aduncum

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Development of sperm cryopreservation protocol of endangered spiny eel, Mastacembelus armatus (Lacepede 1800) for ex-situ conservation

Cryobiology ◽

10.1016/j.cryobiol.2016.10.004 ◽

2016 ◽

Vol 73 (3) ◽

pp. 316-323 ◽

Cited By ~ 9

Author(s):

M. Mahfujur Rahman ◽

M. Rahmat Ali ◽

M. Rafiqul Islam Sarder ◽

M. Fazlul Awal Mollah ◽

Najmus Sakib Khan

Keyword(s):

Ex Situ Conservation ◽

Ex Situ ◽

Sperm Cryopreservation ◽

Mastacembelus Armatus ◽

Cryopreservation Protocol

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Cryopreservation of spermatozoa of indigenous stinging catfish, Heteropneustes fossilis (Bloch) for ex-situ conservation

Bangladesh Journal of Fisheries ◽

10.52168/bjf.2020.32.02 ◽

2020 ◽

Vol 32 (1) ◽

pp. 11-22

Author(s):

MD. RAFIQUL ISLAM SARDER ◽

MD. ABUL KALAM AZAD ◽

K.M. SHAHRIAR NAZRUL ◽

MOHAMMAD RASHED

Keyword(s):

Ex Situ Conservation ◽

Egg Yolk ◽

Ringer Solution ◽

The Other ◽

Ex Situ ◽

Nacl Solution ◽

Heteropneustes Fossilis ◽

Significant Difference ◽

Cryopreserved Sperm ◽

Cryopreservation Protocol

An experiment was conducted to develop cryopreservation protocol for spermatozoa of stinging catfish, Heteropneustes fossilis and to use the cryopreserved sperm in its breeding trials. The activation of sperm motility at various concentrations of NaCl solution was tested and complete activation and inhibition of sperm were obtained at 0.4% and 0.9% to 1% NaCl solution respectively. In toxicity test, sperms were incubated with DMSO, methanol and ethanol at 5, 10, and 15% concentrations where DMSO and methanol produced better motility at 5 and 10% concentration with Alsever’s solution and egg-yolk citrate at 5 and- 10 min incubation period. Three extenders- Alsever’s solution, egg-yolk citrate and Ginsburg Fish Ringer solution and three cryoprotectants- DMSO, methanol and ethanol were used for cryopreservation of sperm, and among the diluents, Alsever’s solution with 10% DMSO showed best performance producing 77.50±3.22% post-thaw motility. On the other hand, egg-yolk citrate and Ginsburg Fish Ringer solution along with 10% DMSO produced 63.75±2.39% and 62.50±3.22% post-thaw motility, respectively. Sperm preserved with Alsever’s solution plus 10% DMSO produced 52.5±3.34% and 38.0±2.39%fertilization and hatching, and those preserved with Alsever’s solution plus 10% methanol produced 46.9±3.11% and 32.7±2.70% fertilization and hatching respectively. The fry produced using cryopreserved and fresh sperm grew well and no significant difference (p>0.05) was found between two groups.

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Ex-situ conservation of medicinal plant genetic resources managed by national agrobiodiversity center of Korea

Planta Medica ◽

10.1055/s-0032-1320321 ◽

2012 ◽

Vol 78 (11) ◽

Author(s):

JS Sung ◽

CW Jeong ◽

YY Lee ◽

HS Lee ◽

YA Jeon ◽

...

Keyword(s):

Medicinal Plant ◽

Genetic Resources ◽

Ex Situ Conservation ◽

Plant Genetic Resources ◽

Ex Situ

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The Nagoya Protocol and the Role of Research Resource Centers as Ex-situ Conservation Institutes

Journal of Environmental Policy and Administration ◽

10.15301/jepa.2018.26.2.47 ◽

2018 ◽

Vol 26 (2) ◽

pp. 47-69

Author(s):

Hyeyeon Im ◽

Minkyung Jung ◽

Kyungsook Ahn ◽

Ki Hyun Ryu

Keyword(s):

Ex Situ Conservation ◽

Ex Situ ◽

Nagoya Protocol

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Ex Situ Conservation of Rare Medicinal Plants in the Botanical Garden (Institute) of Academy of Sciences of Moldova

Acta Horti Botanici Bucurestiensis ◽

10.2478/ahbb-2013-0005 ◽

2013 ◽

Vol 40 (1) ◽

pp. 43-48

Author(s):

Nina Ciocârlan

Keyword(s):

Native Species ◽

Ex Situ Conservation ◽

Botanical Garden ◽

Ex Situ ◽

Aromatic Plants ◽

Medicinal And Aromatic Plants ◽

Field Collection ◽

Ecological Flexibility ◽

Cultivation Techniques ◽

Academy Of Sciences

Abstract This work refers to the native species of genus Astragalus L. (A. dasyanthus, A. ponticus), Adonis L. (A. vernalis, A. wolgensis) and Digitalis L. (D. lanata, D. grandiflora). The plants are cultivated in the Botanical Garden of Moldova in the field collection of the medicinal and aromatic plants. Investigation includes propagation aspects, research into cultivation techniques and conservation measures. The biological particularities and the phenologic rhythm are also registered. The obtained data shows the ecological flexibility of species and the possibility of preserving them in culture.

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