Verification of the resistance of a LEA gene from Tamarix expression in Saccharomyces cerevisiae to abiotic stresses

2008 ◽  
Vol 19 (1) ◽  
pp. 58-62 ◽  
Author(s):  
Bing-feng Wang ◽  
Yu-cheng Wang ◽  
Da-wei Zhang ◽  
Hong-yan Li ◽  
Chuan-ping Yang
2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Xiaoli Tang ◽  
Hongyan Wang ◽  
Liye Chu ◽  
Hongbo Shao

The LEA proteins are a kind of hydrophilic proteins, playing main functions in desiccation tolerance. However, their importance as a kind of stress proteins in abiotic stress is being clarified little by little. In this study we isolated, cloned, and identified the firstKvLEAgene inKosteletzkya virginica. Bioinformatic analysis showed that the protein encoded by this gene had common properties of LEA proteins and the multiple sequences alignment and phylogenetic analysis further showed that this protein had high homology with twoArabidopsisLEA proteins. Gene expression analysis revealed that this gene had a higher expression in root and it was induced obviously by salt stress. Moreover, the transcripts ofKvLEAwere also induced by other abiotic stresses including drought, high temperature, chilling, and ABA treatment. Among these abiotic stresses, ABA treatment brought about the biggest changes to this gene. Collectively, our research discovered a novel LEA gene and uncovered its involvement in multiabiotic stresses inK. virginica. This research not only enriched studies on LEA gene in plant but also would accelerate more studies onK. virginicain the future.


Author(s):  
Annu Yadav ◽  
Himanshi . ◽  
Shruti . ◽  
Jitender Singh ◽  
Pankaj Kumar ◽  
...  

Plants during their growth, experience periodic stress conditions both abiotic (adverse environmental conditions) as well as biotic (infection by pathogens). They appear to respond to these adverse conditions by modulating the expression of many genes. One of the pronounced effects of stress on plant is the enhanced synthesis of a set of proteins-termed ' stress proteins'. Lentil contains asset of genes/proteins which helps this crop to overcome abiotic stresses. In the present study, HSP70 (Heat Shock Protein), LEA (Late Embryogenesis Abundant) and Aldolase genes were identified and cloned in pTZ57RT vector followed by sequencing. Expression analysis was done through Q-PCR which was assessed by using cDNA from all the heat, drought and salinity stressed and unstressed lentil cotyledons. The highest level of transcript of HSP70 was realized upon exposure to heat at 45°C for 3 hour followed by at 45°C for 2 hour and lowest at 40°C for 1hour. LEA gene was identified under drought and salinity stress and highest transcript was at 20% PEG for 3 hour (drought stress) and in salinity stress highest transcript was at 150 mm for 6 hour.  For Aldolase gene highest transcript was recorded after 3, 6 and 12 hr at 100 mM, 150 mM, 200 mM of salinity stress respectively.  From these studies it can be concluded that heat shock protein gene, LEA, and aldolase present in lentil which can be exploited in overcoming the abiotic stresses for obtaining the higher productivity in crop plants through genetic engineering.


2010 ◽  
Vol 167 (14) ◽  
pp. 1172-1178 ◽  
Author(s):  
Hongmiao Song ◽  
Pengxiang Fan ◽  
Wuliang Shi ◽  
Rongmin Zhao ◽  
Yinxin Li

2001 ◽  
Vol 36 (2) ◽  
pp. 196-201 ◽  
Author(s):  
F. Seibold ◽  
O. Stich ◽  
R. Hufnagl ◽  
S. Kamil ◽  
M. Scheurlen

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