ABSTRACTObjective: In this study, silver nanoparticles (SNPs) were synthesized using an aqueous extract of Trainthema portulacastrum and silver ions (Ag+)which have been proven against certain pathogenic bacterial strains and hepatocellular carcinoma (HepG2) cell line.Methods: The bio fabricated nanoparticles were confirmed by surface plasmon resonance which were characterized by biophysical measuresutilizing the ultraviolet-visible spectroscopy, scanning electron microscopy (SEM), energy dispersive X-ray, and transmission electron microscope(TEM), Fourier transform infrared spectroscopy, particle size analyzer, and X-ray diffraction. Antibacterial efficacy against Enterobacter aerogens,Proteus mirabilis, Escherichia coli, Staphylococcus epidermis, and Bacillus subtilis. The effect of SNPs tested against HepG2 and NIH/3T3 cell lineexhibits a dose-dependent toxicity.Results and Conclusion: The SEM and TEM images confirmed the presence of spherical and hexagonal shape (0.3-4 μm) of nanocrystalline particleswith the size range of 11.5-29.2 nm. The average particles size of SNPs is 190.3±17.0 nm. Antibacterial activity was carried out by agar well diffusionmethod against different pathogenic bacteria of which B. subtilis showed a significant zone of inhibition 8.66 mm and 12.0 mm for aqueous plantextract and synthesized SNPs. The effect of SNPs tested against HepG2 and NIH/3T3 cell line exhibits a dose-dependent toxicity. In case of HepG2, thecell viability was decreased to 50% (IC50) at the concentration of 173.8±0.84 μg/mL. From the results, it can be concluded that the SNPs fabricatedusing green synthesis method will be a promising candidate in the biomedical field, due to its high bioactive properties.Keywords: Silver nanoparticles, Trainthema portulacastrum, Antibacterial activity, Cytotoxic activity.
SILVER NANOPARTICLES FROM TRIANTHEMA PORTULACASTRUM: GREEN SYNTHESIS, CHARACTERIZATION, ANTIBACTERIAL AND ANTICANCER PROPERTIES