scholarly journals Antibacterial activity of royal jelly-mediated green synthesized silver nanoparticles

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Susanna Gevorgyan ◽  
Robin Schubert ◽  
Mkrtich Yeranosyan ◽  
Lilit Gabrielyan ◽  
Armen Trchounian ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Royal Jelly ◽  
Fluorescence Emission ◽  
Spherical Particles ◽  
Ag Nps ◽  
Gram Negative ◽  
Green Synthesized Silver Nanoparticles ◽  
Higher Sensitivity

AbstractThe application of green synthesis in nanotechnology is growing day by day. It’s a safe and eco-friendly alternative to conventional methods. The current research aimed to study raw royal jelly’s potential in the green synthesis of silver nanoparticles and their antibacterial activity. Royal jelly served as a reducing and oxidizing agent in the green synthesis technology of colloidal silver nanoparticles. The UV–Vis maximum absorption at ~ 430 nm and fluorescence emission peaks at ~ 487 nm confirmed the presence of Ag NPs. Morphology and structural properties of Ag NPs and the effect of ultrasound studies revealed: (i) the formation of polydispersed and spherical particles with different sizes; (ii) size reduction and homogeneity increase by ultrasound treatment. Antibacterial activity of different concentrations of green synthesized Ag NPs has been assessed on Gram-negative S. typhimurium and Gram-positive S. aureus, revealing higher sensitivity on Gram-negative bacteria.

scholarly journals GREEN SYNTHESIS OF SILVER NANOPARTICLES USING MANGROVE FRUIT POLYSACCHARIDE FOR BACTERIAL GROWTH INHIBITION

2019 ◽  
pp. 179-183 ◽  
Author(s):  
GAJENDRA NATH MAITY ◽  
JOY SARKAR ◽  
SOMANJANA KHATUA ◽  
SOUMITRA MONDAL ◽  
KRISHNENDU ACHARYA
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Physicochemical Characterization ◽  
Gas Chromatography Mass Spectrometry ◽  
Ag Nps ◽  
Gram Positive ◽  
Mangrove Plant ◽  
Gram Negative ◽  
Plant Polysaccharide

Objective: The objective of this study was to find out the antibacterial activity of the silver nanoparticles (Ag-NPs) using a low-cost green synthesis approach for the formulation of Ag-NPs applying polysaccharide extracted from the fruits of a mangrove plant of Sundarban. Methods: Fresh and healthy fruits were collected from Ceriops decandra plant. Sufficient amount of carbohydrates was extracted from those fruits and the physicochemical characterization of the polysaccharide was analyzed by gas chromatography–mass spectrometry and Fourier-transform infrared spectrophotometry. The respective polysaccharide was further applied to generate the Ag-NPs which were characterized by UV visible, dynamic light scattering, transmission electron microscopy, EDAX, and X-ray diffraction. The antibacterial efficacy of the Ag-NPs was also determined against some pathogenic Gram-negative and Gram-positive bacteria using the microdilution method. Results: Glucose and galactose are the major monomers among the extracted carbohydrates. Various types of spectral analysis confirmed the formation of Ag-NPs. The green synthesized Ag-NPs have the average diameter of about 28 nm. Furthermore, the green synthesized Ag-NPs exhibited strong antibacterial activity against some pathogenic Gram-positive (L. cytomonogenes, Bacillus Subtilis, and Staphylococcus aureus) and Gram-negative (Salmonella typhimurium and Escherichia coli) bacteria. Conclusion: The green synthesis of Ag-NPs using plant polysaccharide was an environment-friendly and cost-effective method as compared to the conventional physical and chemical synthesis techniques.

scholarly journals Bio fabrication of silver nanoparticles with antibacterial and cytotoxic abilities using lichens

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Mona A. Alqahtani ◽  
Monerah R. Al Othman ◽  
Afrah E. Mohammed
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Synergistic Effect ◽  
Cancer Cell ◽  
Spherical Particles ◽  
Diffusion Method ◽  
Gram Positive ◽  
Gram Negative ◽  
Vis Spectroscopy ◽  
Hct 116

Abstract Recently, increase bacterial resistance to antimicrobial compounds issue constitutes a real threat to human health. One of the useful materials for bacterial control is Silver nanoparticles (AgNPs). Researchers tend to use biogenic agents to synthesize stable and safe AgNPs. The principal aim of this study was to investigate the ability of lichen in AgNPs formation and to find out their suppression ability to MDR bacteria as well as their cytotoxic activity. In the current study, lichens (Xanthoria parietina, Flavopunctelia flaventior) were collected from the south of the Kingdom of Saudi Arabia. Lichens methanolic extracts were used for conversion of Ag ions to AgNPs. Prepared biogenic AgNPs were characterized by Ultraviolet–Visible (UV–Vis) Spectroscopy, Transmission electron microscopy (TEM), Dynamic Light Scattering (DLS) and Zeta potential and Energy-Dispersive X-ray Spectroscopy (EDS). Lichens Secondary metabolites were determined by Fourier-Transform Infrared Spectroscopy (FTIR) and Gas Chromatography–Mass Spectrometry (GC–MS). The antibacterial activity and synergistic effect of AgNPs were evaluated against pathogenic bacteria, including gram-positive; Methicillin-resistant Staphylococcus aureus (MRSA), Vancomycin-resistant Enterococcus (VRE), and gram-negative; (Pseudomonas aeruginosa, Escherichia coli) as well as the reference strains (ATCC) using the agar disk diffusion method. Cytotoxic effect of biogenic AgNPs was tested against HCT 116 (Human Colorectal Cancer cell), MDA-MB-231 (Breast cancer cell), and FaDu (Pharynx cancer cell) by MTT test. TEM imaging showed well-dispersed spherical particles of 1–40 nm size as well as zeta size showed 69–145 nm. Furthermore, FTIR and GC–MS identified various lichen chemical molecules. On the other hand, the highest antibacterial activity of AgNPs was noticed against P. aeruginosa, followed by MRSA, VRE, and E. coli. AgNPs influence on gram-negative bacteria was greater than that on gram-positive bacteria and their synergistic effect with some antibiotics was noted against examined microbes. Moreover, higher cytotoxicity for biogenic AgNPs against FaDu and HCT 116 cell line in relation to MDA-MB-231 was noted. Given the current findings, the biogenic AgNPs mediated by lichens had positive antibacterial, synergistic and cytotoxic powers. Therefore, they might be considered as a promising candidate to combat the multi-drug resistance organisms and some cancer cells.

scholarly journals Green Synthesis of Silver Nanoparticles Using Novel Launaea Procumbens Leaves Extract and Screening Its Antibacterial Activity

2021 ◽  
Author(s):  
Sanjay Ratan Kumavat ◽  
SATYENDRA MISHRA
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Gram Negative Bacteria ◽  
Zone Of Inhibition ◽  
Gram Negative ◽  
Green Synthesized Silver Nanoparticles ◽  
Launaea Procumbens

Abstract Plants are emerging as a cost-effective and ecofriendly method for green synthesis of nanoparticles. The plant extract Launaea procumbens was used as a reduction agent in the green synthesis of silver nanoparticles. UV-Visible spectroscopy, HR-TEM, SAED, FE-SEM, EDAX, DLS, and FT-IR were used to study the green synthesized silver nanoparticles. UV-Vis spectroscopy of a prepared silver solution revealed maximum absorption at 435 nm. The synthesized silver nanoparticles were found to be spherical in shape with a size in the range of 24.28 to 31.54 nm. DLS analysis was used to determine the size of the green synthesized silver nanoparticles, which showed outstanding antibacterial action against Gram-positive bacteria Bacillus subtilis and Staphylococcus aureus, as well as Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa. Gram-positive Bacillus subtilis had a maximum zone of inhibition of 20 mm, Staphylococcus aureus had a zone of inhibition of 19 mm, and Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa had zones of inhibition of 13 mm.

scholarly journals Green synthesis of bio-molecule encapsulated magnetic silver nanoparticles and their antibacterial activity

RSC Advances ◽  
2018 ◽  
Vol 8 (65) ◽  
pp. 37176-37183 ◽  
Author(s):  
A. K. M. Atique Ullah ◽  
M. F. Kabir ◽  
M. Akter ◽  
A. N. Tamanna ◽  
A. Hossain ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Pathogenic Bacteria ◽  
Ag Nps ◽  
Synthesis Technique

Persuaded by the necessity of finding new sources of antibiotics, Ag NPs were synthesized by adopting a newly developed green synthesis technique and subsequently, their antibacterial activity against different pathogenic bacteria was evaluated.

Green synthesis of silver nanoparticles from Psidium guajava leaves and its antibacterial activity

2017 ◽  
Vol 6 (7) ◽  
pp. 5441 ◽  
Author(s):  
Geetha Venugopal
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Plant Extract ◽  
Psidium Guajava ◽  
Zone Of Inhibition ◽  
Guava Leaf ◽  
Green Synthesized Silver Nanoparticles

In the present study, Psidium guajava leaves were taken for synthesizing silver nanoparticles and checked their antibacterial activity against E.coli, Klebsiella, Pseudomonas, Staphylococcus and Acinetobacter. The plant extract was analysed for the detection of the presence of protein, carbohydrate, flavonoids, terpenoids, glycosides, steroids, saponins, phenols and tannins. In this present study, the antibacterial activity of green synthesized silver nanoparticles from guava leaf shows the zone of inhibition against all the five pathogens.

scholarly journals Green Synthesis of Gold and Silver Nanoparticles by Using Amorphophallus paeoniifolius Tuber Extract and Evaluation of Their Antibacterial Activity

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4773
Author(s):  
S. M. Abu Nayem ◽  
Nasrin Sultana ◽  
Md. Aminul Haque ◽  
Billal Miah ◽  
Md. Mahmodul Hasan ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Inhibitory Effect ◽  
Reducing Agent ◽  
Gram Negative Bacteria ◽  
Gold And Silver Nanoparticles ◽  
Gram Negative ◽  
X Ray ◽  
Tuber Extract

In this report, we discussed rapid, facile one-pot green synthesis of gold and silver nanoparticles (AuNPs and AgNPs) by using tuber extract of Amorphophallus paeoniifolius, and evaluated their antibacterial activity. AuNPs and AgNPs were synthesized by mixing their respective precursors (AgNO3 and HAuCl4) with tuber extract of Amorphophallus paeoniifolius as the bio-reducing agent. Characterization of AuNPs and AgNPs were confirmed by applying UV-vis spectroscopy, field-emission scanning electron microscopy (FESEM), X-ray diffraction (XRD) analysis, Fourier transform infrared spectroscopy (FTIR), and energy dispersive X-ray spectroscopy (EDS). From UV-vis characterization, surface plasmon resonance spectra were found at 530 nm for AuNPs and 446 nm for AgNPs. XRD data confirmed that both synthesized nanoparticles were face-centered cubic in crystalline nature, and the average crystallite sizes for the assign peaks were 13.3 nm for AuNPs and 22.48 nm for AgNPs. FTIR data evaluated the characteristic peaks of different phytochemical components of tuber extract, which acted as the reducing agent, and possibly as stabilizing agents. The antibacterial activity of synthesized AuNPs and AgNPs were examined in Muller Hinton agar, against two Gram-positive and four Gram-negative bacteria through the disc diffusion method. AuNPs did not show any inhibitory effect, while AgNPs showed good inhibitory effect against both Gram-positive and Gram-negative bacteria.

scholarly journals Green Sonochemical Synthesis of Silver Nanoparticles Using Adansonia Digitata Leaves Extract and Evaluation of Their Antibacterial Potential

2020 ◽  
Vol 1 (2) ◽  
Author(s):  
Wilson Mbiti Njue ◽  
Jackson Kilonzo Kithokoi ◽  
Jane Mburu ◽  
Henry Mwangi ◽  
Sauda Swaleh
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Metal Nanoparticles ◽  
Pathogenic Bacteria ◽  
Cost Effective ◽  
Adansonia Digitata ◽  
Gram Negative ◽  
Selected Area Electron Diffraction ◽  
Average Size

Metal nanoparticles in the field of nanotechnology are of great interest to modern scientific research due to their size effects, medical uses and, catalytic, electronic and optical properties. Green synthesis of metal nanoparticles is a feasible alternative to chemical methods as it is environmentally friendly and cost effective. In continuation with our research on green synthesis of silver nanoparticles using Kenyan medicinal plants, we here report the synthesis of novel silver nanoparticles (AgNPs) on ultrasonic bath using Adansonia digitata leaves extracts and analysis of their antibacterial activity. The nanoparticles were characterized by UV-Vis, High Resolution Transmission Electron Microscopy (HRTEM), FTIR spectroscopy and Energy Dispersive X-ray (EDX). EDX analysis affirmed the nanoparticles were pure silver. Crystalline nature of the nanoparticles was confirmed by bright circular spots in the Selected Area Electron Diffraction (SAED) in HRTEM image. The AgNPs were spherical with an average size 13 nm. FTIR analysis showed strong –C=C- and –OH stretching bands due to compounds capping the nanoparticles. The synthesized AgNPs showed high inhibition zones of 17.1±0.130 mm towards Gram-negative bacteria E. coli and 12.9±0.082mm towards Gram positive bacteria S. aureus. The aqueous A. digitata extract had no effect on growth inhibition of test bacteria. The study showed that the silver nanoparticles synthesized from the plant’s leaves extract had antibacterial activity against both Gram negative and positive pathogenic bacteria. The nanoparticles can be utilized towards developing novel drugs useful in combating pathogens.

Green Synthesis of Silver Nanoparticles using Datura metel Flower Extract Assisted by Ultrasound Method and Its Antibacterial Activity

2021 ◽  
Vol 15 ◽  
Author(s):  
Is Fatimah ◽  
Habibi Hidayat ◽  
Bambang Nugroho ◽  
Saddam Husein
Keyword(s):  
Particle Size ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Ag Nps ◽  
Ultrasound Method ◽  
Flower Extract ◽  
Datura Metel ◽  
Uv Visible ◽  
Ultrasound Assisted

Background: Green synthesis method of nanoparticles has been developed for several years. Besides providing an environmental-friendly process, the green synthesis of nanoparticles using plant extract provides a synergistic effect of the secondary metabolite. In recent times, the study involving the intensification process in nanoparticle formation also attracts great attention. This research deals with the green synthesis of silver nanoparticles using Datura metel flower extract as an antibacterial agent. The use of an ultrasound-assisted method for the synthesis is also investigated in this study. Methods: Synthesis of silver nanoparticles (AgNPs) using Datura metel flower extract under ultrasound-assisted method has been conducted. Evaluation of the successful synthesis was done using UV-visible spectrophotometry, particle size analyzer, x-ray diffraction, and transmission electron microscopy. The prepared AgNPs were tested as antibacterial against S. aureus, K. pneumoniae, S. pyogenes, and E. coli. Results: The ultrasound-assisted synthesis of AgNPs produces particles ranging from 25-70 nm in size; meanwhile, the reflux method demonstrated the size of 50-170 nm. These particle sizes represent the effect of the antibacterial activity as the ultrasound-assisted synthesized Ag NPs have a higher inhibition zone towards all tested bacteria. Subsequently, these data presented the applicability of Ag NPs synthesis using an ultrasound method as a potential candidate for biomedical applications. Conclusion: The profile of UV-Visible spectra and particle size analyses demonstrated the applicability of the ultrasound technique to produce a smaller size of the nanoparticles with higher antibacterial activity.

scholarly journals Hydrothermal Green Synthesis of Silver Nanoparticles using Azadirachta indica A. Juss. Fruit Juice for Potential Antibacterial Activity

2018 ◽  
Vol 4 (5) ◽  
pp. 519-523
Author(s):  
K. Ramar ◽  
A. Jafar Ahamed
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Azadirachta Indica ◽  
Ag Nps ◽  
Gram Positive ◽  
Uniform Size ◽  
Gram Negative ◽  
Agar Disc ◽  
Mueller Hinton Agar ◽  
Average Size

This study employed a green, eco-friendly and convenient hydrothermal method for the synthesis of stable silver nanoparticles (Ag NPs) using neem (Azadirachta indica A Juss) fruit extract, which also acts as a reducing and capping agents. Since neem fruits has been found to have various medicinal applications, this fruit has been chosen in this paper to obtain Ag NPs. The Ag NPs were characterized by PXRD, UV–Vis diffuse reflectance spectra (DRS), FTIR, FESEM-EDAX, and HRTEM analysis. UV–Vis DRS spectra studies provided sufficient evidences for the formation of Ag NPs. The major role of functional groups present in the synthesis and stabilization of the NPs was obtained from FTIR studies. The PXRD and HRTEM investigations clearly demonstrated the crystalline nature of the NPs. From the HRTEM images, the Ag NPs were found to be spherical and of nearly uniform size with an average size of 13 nm. Further, Ag NPs were subjected to the agar disc diffusion antimicrobial assay (Mueller–Hinton Agar testing) against Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) bacteria. The test results showed the potent antibacterial activity of NPs for Gram-positive bacteria is higher than that the Gram- negative bacteria

scholarly journals Silver nanoparticles: Green Synthesis and Their Antibacterial efficiency

2021 ◽  
Vol 59 (2) ◽  
pp. 214
Author(s):  
Dung Ngo Thanh ◽  
Nguyet Ha Minh ◽  
Tam Le Thi Thanh ◽  
Lu Le Trong
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Silver Nitrate ◽  
Tannic Acid ◽  
Ag Nanoparticles ◽  
Sodium Citrate ◽  
Reducing Agents ◽  
Ag Nps ◽  
Silver Nps

In this study, silver nanoparticles were synthesized from aqueous silver nitrate through a simple and eco-friendly route using a combination of two reducing agents: sodium citrate and tannic acid. By this method, the obtained Ag nanoparticles (NPs) were stable within the studied period of six months. Besides, both TEM images and UV-Vis results showed that the size of silver NPs could be controlled by changing the concentration of tannic acid. The antibacterial ability of Ag NPs with different sizes were also examined. In detail, the smaller the Ag NPs were, the more efficient their antibacterial activity was.

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