Cold-hardening of winter triticale (x Triticosecale Wittm.) results in increased resistance to pink snow mould Microdochium nivale (Fr., Samuels & Hallett) and genotype-dependent chlorophyll fluorescence modulations

2009 ◽  
Vol 31 (6) ◽  
pp. 1219-1227 ◽  
Author(s):  
Gabriela Gołębiowska ◽  
Maria Wędzony
Keyword(s):  
Chlorophyll Fluorescence ◽  
Microdochium Nivale ◽  
Cold Hardening ◽  
Winter Triticale ◽  
X Triticosecale ◽  
Pink Snow Mould ◽  
Snow Mould

scholarly journals Cold-modulated small proteins abundance in winter triticale (x Triticosecale, Wittm.) seedlings tolerant to the pink snow mould (Microdochium nivale, Samuels & Hallett) infection

2019 ◽  
Author(s):  
Gabriela Julia Golebiowska ◽  
Emilia Bonar ◽  
Kaveh Emami ◽  
Maria Wędzony
Keyword(s):  
Small Subunit ◽  
Two Dimensional Gel Electrophoresis ◽  
Winter Triticale ◽  
X Triticosecale ◽  
Small Proteins ◽  
Pink Snow Mould ◽  
Snow Mould ◽  
Quantitative Changes ◽  
Triticosecale Wittmack ◽  
The Impact

Two winter triticale (x Triticosecale Wittmack) model cultivars: Hewo (tolerant to pink snow mould) and Magnat (sensitive) were used to test the effect of cold-hardening (4 weeks at 4°C) on soluble ≤50 kDa protein profiles of the seedling leaves. The presence and abundance of individual proteins were analysed via two-dimensional gel electrophoresis (2-DE) and Surface-Enhanced Laser Desorption/Ionization Time-of-Flight (SELDI-TOF). Up to now, no proteomics analysis of triticale response to hardening has been performed. Thus, the present paper is the first in the series describing the obtained results. In our experiments, the exposure to the low temperature-induced only quantitative changes in the leaves of both cultivars, causing either an increase or decrease of 4–50 kDa protein abundance. Among proteins which were cold-accumulated in cv. Hewo’s leaves, we identified two thioredoxin peroxidases (chloroplastic thiol-specific antioxidant proteins) as well as mitochondrial- β-ATP synthase subunit and ADP-binding resistance protein. On the contrary, in hardened seedlings of this genotype, we observed the decreased level of chloroplastic RuBisCO small subunit PW9 and epidermal peroxidase 10. Simultaneous SELDI-TOF analysis revealed several low mass proteins better represented in cold-hardened plants of tolerant genotype in comparison to the sensitive one and the impact of both genotype and temperature on their level. Based on those results, we suggest that indicated proteins might be potential candidates for molecular markers of cold-induced snow mould resistance of winter triticale and their role is worth to be investigated in the further inoculation experiments.

β-1,3-glucanase and chitinase activities in winter triticales during cold hardening and subsequent infection by Microdochium nivale

Biologia ◽  
2013 ◽  
Vol 68 (2) ◽  
Author(s):  
Iwona Żur ◽  
Gabriela Gołębiowska ◽  
Ewa Dubas ◽  
Elżbieta Golemiec ◽  
Ildikó Matušíková ◽  
...  
Keyword(s):  
Microdochium Nivale ◽  
Fungal Mycelium ◽  
Subsequent Infection ◽  
Winter Triticale ◽  
Pathogenesis Related ◽  
Altered Metabolism ◽  
Snow Mould ◽  
Related Proteins ◽  
Cold Acclimatisation ◽  
Snow Mould Resistance

AbstractThe accumulation of pathogenesis-related proteins such as β-1,3-glucanases and chitinases was studied in cold induced snow mould resistance in two Polish cultivars of winter triticale, cv. Hewo and cv. Magnat that substantially differ in resistance to Microdochium nivale. The plants were pre-hardened at 12°C for 10 days and hardened at 4°C for 28 days. Subsequently, cold hardened plants were inoculated with fungal mycelium (M. nivale) and incubated at 4°C for 7 days in dark. Cold acclimatisation resulted in suppression of the total glucanase and chitinases activities in the resistant Hewo as well as sensitive Magnat cultivars that possibly coincides with altered metabolism. However, upon infection with M. nivale the chitinases were markedly induced in the cv. Hewo. At the same time, total β-1,3 glucanases activities did not seem to be affected by fungus in any of the tested triticale cultivars. The pattern and/or the activity of chitinases in plants might be indicative for the resistance/susceptibility against M. nivale.

scholarly journals Quantitative Trait Loci and Candidate Genes Associated with Cold-Acclimation and Microdochium nivale Tolerance/Susceptibility in Winter Triticale (x Triticosecale)

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2678
Author(s):  
Gabriela Gołębiowska ◽  
Mateusz Dyda ◽  
Katarzyna Wajdzik
Keyword(s):  
Candidate Genes ◽  
Quantitative Trait ◽  
Doubled Haploids ◽  
Damage Index ◽  
Microdochium Nivale ◽  
Plant Tolerance ◽  
Linkage Groups ◽  
F1 Hybrid ◽  
Winter Triticale ◽  
X Triticosecale

Tolerance to pink snow mold caused by Microdochium nivale appears after a cold-hardening period and it is an essential, genotype-dependent, complex quantitative trait for the wintering of triticale (x Triticosecale) and other cereals. Despite long-term studies, a marker for the selection of the tolerant genotypes is still insufficiently recognized. Chlorophyll fluorescence has been reported as a sensitive indicator of stress effects on photosynthesis and can be used to predict plant tolerance. In this study, the genomic regions (QTLs) associated with the level of winter triticale seedlings damage caused by M. nivale infection as well as photosynthesis quantum efficiency and chlorophyll a fluorescence parameters were identified in seedlings of mapping population of 89 doubled haploids lines (DHs) derived from F1 hybrid of cv. ‘Hewo’ and cv. ‘Magnat’ accompanied with the genetic map consisting of 20 linkage groups with a total map length 4997.4 cm. Independent experiments performed in controlled conditions revealed 13 regions identified by a composite interval mapping, located on 7A, 1B, 2B, 6B, 7B, 3R, 5R, and 6R linkage groups and related to the PI, PIABS, TRo/CS, ABS/CS, ABS/CSm, ABS/RC, and Qy values as well as M. nivale tolerance T and susceptibility level P expressed by the seedling damage index. Additionally, candidate genes were in silico identified with the sequence position on wheat (2B and 7B) and rye (5R) chromosomes, where relevant QTL regions were found. The most important candidate genes indicated for M. nivale tolerance of cold-hardened triticale seedlings include those coding: sterol 3-beta-glucosyltransferase UGT80A2-like, transcription factor NAI1-like, and flavonol3-sulfotransferase-like proteins on chromosomes 2B and 5R.

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