Conjugated Linoleic Acid (t-10, c-12) Reduces Fatty Acid Synthesis de Novo, but not Expression of Genes for Lipid Metabolism in Bovine Adipose Tissue ex Vivo

We determined the effects of leukemia inhibitory factor (LIF) and ciliary neurotrophic factor (CNTF) on lipid metabolism in intact rats. Administration of LIF and CNTF increased serum triglycerides in a dose-dependent manner with peak values at 2 h. The effects of LIF and CNTF on serum cholesterol were very small, and serum glucose was unaffected. Both LIF and CNTF stimulated hepatic triglyceride secretion, hepatic de novo fatty acid synthesis, and lipolysis. Pretreatment with phenylisopropyl adenosine, which inhibits lipolysis, partially inhibited LIF- and CNTF-induced hypertriglyceridemia. Interleukin-4, which inhibits cytokine-induced hepatic fatty acid synthesis, also partially inhibited LIF- and CNTF-induced hypertriglyceridemia. These results indicate that both lipolysis and de novo fatty acid synthesis play a role in providing fatty acids for the increase in hepatic triglyceride secretion. Neither indomethacin nor adrenergic receptor antagonists affected the hypertriglyceridemia. The combination of LIF plus CNTF showed no additive effects consistent with the action of both cytokines through the gp130 transduction system. Thus LIF and CNTF have similar effects on lipid metabolism; they join a growing list of cytokines that stimulate hepatic triglyceride secretion and may mediate the changes in lipid metabolism that accompany the acute phase response.

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Alternative pathway of free fatty acid uptake from phospholipids and upregulation of de novo fatty acid synthesis in lipoprotein lipase-deficient murine adipose tissue

Atherosclerosis Supplements ◽

10.1016/s1567-5688(02)80369-5 ◽

2002 ◽

Vol 3 (2) ◽

pp. 145

Keyword(s):

Adipose Tissue ◽

Fatty Acid ◽

Free Fatty Acid ◽

Lipoprotein Lipase ◽

Fatty Acid Synthesis ◽

De Novo ◽

Alternative Pathway ◽

Acid Synthesis ◽

Fatty Acid Uptake ◽

Acid Uptake

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Downregulation of de Novo Fatty Acid Synthesis in Subcutaneous Adipose Tissue of Moderately Obese Women

International Journal of Molecular Sciences ◽

10.3390/ijms161226206 ◽

2015 ◽

Vol 16 (12) ◽

pp. 29911-29922 ◽

Cited By ~ 8

Author(s):

Esther Guiu-Jurado ◽

Teresa Auguet ◽

Alba Berlanga ◽

Gemma Aragonès ◽

Carmen Aguilar ◽

...

Keyword(s):

Adipose Tissue ◽

Fatty Acid ◽

Fatty Acid Synthesis ◽

Subcutaneous Adipose Tissue ◽

De Novo ◽

Acid Synthesis ◽

Obese Women ◽

Subcutaneous Adipose

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Taurine-Mediated IDOL Contributes to Resolution of Streptococcus uberis Infection

Infection and Immunity ◽

10.1128/iai.00788-20 ◽

2021 ◽

Vol 89 (5) ◽

Author(s):

Zhixin Wan ◽

Riguo Lan ◽

Yilin Zhou ◽

Yuanyuan Xu ◽

Zhenglei Wang ◽

...

Keyword(s):

Fatty Acid ◽

Lipid Metabolism ◽

Fatty Acid Synthesis ◽

De Novo ◽

Bacterial Load ◽

Critical Role ◽

Acid Synthesis ◽

Content Type ◽

Streptococcus Uberis

ABSTRACT Metabolic alterations occur in pathogenic infections, but the role of lipid metabolism in the progression of bacterial mastitis is unclear. Cross talk between lipid droplets (LDs) and invading bacteria occurs, and targeting of de novo lipogenesis inhibits pathogen reproduction. In this study, we investigate the role(s) of lipid metabolism in mammary cells during Streptococcus uberis infection. Our results indicate that S. uberis induces the synthesis of fatty acids and production of LDs. Importantly, taurine reduces fatty acid synthesis, the abundance of LDs and the in vitro bacterial load of S. uberis. These changes are mediated, at least partly, by the E3 ubiquitin ligase IDOL, which is associated with the degradation of low-density lipoprotein receptors (LDLRs). We have identified a critical role for IDOL-mediated fatty acid synthesis in bacterial infection, and we suggest that taurine may be an effective prophylactic or therapeutic strategy for preventing S. uberis mastitis.

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Effects of pregnancy and ovarian steroids on fatty acid synthesis and uptake in Syrian hamsters

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.1991.260.1.r153 ◽

1991 ◽

Vol 260 (1) ◽

pp. R153-R158 ◽

Cited By ~ 1

Author(s):

A. J. Bhatia ◽

G. N. Wade

Keyword(s):

Fatty Acids ◽

Adipose Tissue ◽

Fatty Acid ◽

White Adipose Tissue ◽

Fatty Acid Synthesis ◽

De Novo ◽

Acid Synthesis ◽

De Novo Lipogenesis ◽

Ovarian Steroids ◽

Syrian Hamsters

The effects of pregnancy and ovarian steroids on the in vivo distribution of newly synthesized fatty acids (incorporation of tritium from 3H2O into fatty acid) in Syrian hamsters (Mesocricetus auratus) were examined. During late, but not early, gestation hamsters had reduced levels of newly synthesized fatty acids in heart, liver, uterus, and white adipose tissues (parametrial and inguinal fat pads). Treatment of ovariectomized hamsters with estradiol + progesterone significantly decreased fatty acid synthesis-uptake in heart, liver, and inguinal white adipose tissue. Treatment with either estradiol or progesterone alone was without significant effect in any tissue. Pretreatment of hamsters with Triton WR-1339 (tyloxapol), an inhibitor of lipoprotein lipase activity and tissue triglyceride uptake, abolished the effects of estradiol + progesterone in white adipose tissue and heart but not in liver. Thus hamsters lose body fat during pregnancy in part because of decreased de novo lipogenesis. The effect of pregnancy on lipogenesis is mimicked by treatment with estradiol + progesterone but not by either hormone alone. Furthermore, it appears that the liver is the principal site of estradiol + progesterone action on lipogenesis in Syrian hamsters.

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