Genetic and genomic characterization of multidrug resistant Bacillus subtilis M3 isolated from an activated sludge reactor treating wastewater

Biologia ◽  
2022 ◽  
Author(s):  
Hafeez Ul Haq ◽  
Wei Huang ◽  
Ye Li ◽  
Ting Zhang ◽  
Shaocheng Ma ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Activated Sludge ◽  
Multidrug Resistant ◽  
Activated Sludge Reactor ◽  
Genomic Characterization

scholarly journals Genomic Characterization of A Multidrug-resistant Citrobacter freundii Strain ZT01-0079 Co-Producing blaNDM-1 and blaSHV-12 using MiSeq and MinION

2020 ◽  
Author(s):  
Tingyan Zhang ◽  
Yanfeng Lin ◽  
Zhonghong Li ◽  
Xiong Liu ◽  
Jinhui Li ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Southern Blotting ◽  
Multiple Drug Resistance ◽  
Carbapenem Resistance ◽  
Multidrug Resistant ◽  
Multiple Drug ◽  
Citrobacter Freundii ◽  
Rapid Spread ◽  
Genomic Characterization

Abstract Background: The emergence of multi-drug resistant Citrobacter freundii poses daunting challenges to the treatment of clinical infections. The purpose of this study was to characterize the genome of a C. freundii strain with an IncX3 plasmid encoding both the blaNDM-1 and blaSHV-12 genes.Methods: Strain ZT01-0079 was isolated from a clinical urine sample. The Vitek2 system was used for identification and antimicrobial susceptibility testing. The presence of blaNDM-1 was detected by PCR and sequencing. Conjugation experiments and Southern blotting were performed to determine the transferability of the blaNDM-1- carrying plasmid. Nanopore and Illumina sequencing were performed to better understand the genomic characteristics of the strain.Results: Strain ZT01-0079 was identified as C. freundii, and the coexistence of blaNDM-1 and multiple drug resistance genes was confirmed. Electrophoresis and Southern blotting showed that blaNDM-1 was located on a ~53kb IncX3 plasmid. The NDM-1-encoding plasmid was successfully transferred at a frequency of 1.68×10−3. Both blaNDM-1 and blaSHV-12 were located on the self-transferable IncX3 plasmid.Conclusion: The rapid spread of the IncX3 plasmid highlights the importance of continuous monitoring of the prevalence of NDM-1-encoding Enterobacteriaceae. Mutations of existing carbapenem resistance genes will bring formidable challenges to clinical treatment.

scholarly journals First Identification and genomic characterization of multidrug-resistant carbapenemase-producing Enterobacteriaceae clinical isolates in Malawi, Africa

2019 ◽  
Vol 68 (12) ◽  
pp. 1707-1715
Author(s):  
Geoffrey P. Kumwenda ◽  
Yo Sugawara ◽  
Ryuichiro Abe ◽  
Yukihiro Akeda ◽  
Watipaso Kasambara ◽  
...  
Keyword(s):  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Genomic Characterization

scholarly journals Genomic Characterization of Nonclonal mcr-1-Positive Multidrug-Resistant Klebsiella pneumoniae from Clinical Samples in Thailand

2018 ◽  
Vol 24 (4) ◽  
pp. 403-410 ◽  
Author(s):  
Apichai Srijan ◽  
Katie R. Margulieux ◽  
Sirigade Ruekit ◽  
Erik Snesrud ◽  
Rosslyn Maybank ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Multidrug Resistant ◽  
Clinical Samples ◽  
Genomic Characterization

Genomic characterization of multidrug-resistant extraintestinal pathogenic Escherichia coli isolated from grain culture soils

Pedosphere ◽  
2022 ◽  
Vol 32 (3) ◽  
pp. 495-502
Author(s):  
João Pedro Rueda FURLAN ◽  
Inara Fernanda Lage GALLO ◽  
Eliana Guedes STEHLING
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistant ◽  
Pathogenic Escherichia Coli ◽  
Genomic Characterization
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