Purification of a diatom and its identification to Cylindrotheca closterium

2015 ◽  
Vol 14 (2) ◽  
pp. 357-361 ◽  
Author(s):  
Song Wang ◽  
Lin Zhang ◽  
Guanpin Yang ◽  
Baohua Zhu ◽  
Kehou Pan
Keyword(s):  
Cylindrotheca Closterium

The Medium for Intensive Culture of the Diatom Cylindrotheca closterium (Ehrenb.) Reimann et Lewin (Bacillariophyta)

2016 ◽  
Vol 18 (3) ◽  
pp. 279-286 ◽  
Author(s):  
V. I. Ryabushko ◽  
S. N. Zheleznova ◽  
R. G. Gevorgiz ◽  
N. I. Bobko ◽  
A. S. Lelekov
Keyword(s):  
Intensive Culture ◽  
Cylindrotheca Closterium

scholarly journals Production of Surface Active Organic Material and Reduced Sulfur Species During the Growth of Marine Diatom Cylindrotheca closterium

2018 ◽  
Vol 91 (4) ◽  
Author(s):  
Irena Ciglenečki ◽  
Jelena Dautović ◽  
Ana Cvitešić ◽  
Galja Pletikapić
Keyword(s):  
Organic Material ◽  
Marine Diatom ◽  
Sulfur Species ◽  
Cylindrotheca Closterium ◽  
Surface Active ◽  
Reduced Sulfur

scholarly journals Production characteristics of the diatom Cylindrotheca closterium (Ehrenb.) Reimann et Lewin grown in an intensive culture at various nitrogen sources in the medium

2019 ◽  
Vol 4 (1) ◽  
pp. 33-44 ◽  
Author(s):  
S. N. Zheleznova
Keyword(s):  
Stationary Phase ◽  
Nitrogen Source ◽  
Nutrient Medium ◽  
Maximum Yield ◽  
Nitrogen Sources ◽  
Dry Weight ◽  
Sole Source ◽  
Active Growth ◽  
Cylindrotheca Closterium ◽  
Production Characteristics

The diatom Cylindrotheca closterium (Ehrenberg) Reimann et Levin is characterized by high productivity (up to 1.5 g·l-1·day-1) and the ability to accumulate a valuable carotenoid fucoxanthin (up to 2 % of dry weight). In the development of biotechnology based on microalgae, the key issue is the creation of concentrated nutrient medium. Nitrogen is one of the most important components in the nutrient medium that significantly affects the production characteristics of all microalgae. The aim of this study is to compare the production characteristics of C. closterium in an intensive storage culture using different forms of nitrogen in the medium. In the first experiment, nitrate and sodium nitrite, urea, and nitrogen in the form of ammonium were used as a source of nitrogen. The amount of nitrates, nitrites, ammonium, and urea in the medium was calculated from the nitrogen content of the RS nutrient medium, with a nitrogen to phosphorus ratio of 15 : 1. In the second experiment, amino acids were used as a nitrogen source – arginine, asparagine, cysteine. The possibility of using the microalgae C. closterium for the growth of various organic sources of nitrogen (urea, cysteine, asparagine) was shown. Productive characteristics in the intensive storage culture of C. closterium using urea, cysteine, and asparagine as the sole source of nitrogen in the RS nutrient medium were determined. It is shown that when urea was used, the productivity reached its maximum values and amounted to 1.5 g·l-1·day-1. Thus, the expediency of using urea in the medium for obtaining the maximum yield of biomass was shown. The use of cysteine in the stationary phase of growth to achieve a long stationary phase with minimal concentrations of the nitrogen source in the nutrient medium is also advisable. It was found that C. closterium was able to grow and vegetate at sufficiently high concentrations of nitrite, and the addition of nitrogen in ammonium form to the nutrient medium during the active growth of C. closterium led to inhibition of all metabolic processes and to the death of the culture.

scholarly journals The carbon fixation efficiency in biomass of Cylindrotheca closterium (Ehrenberg) Reimann & J. C. Lewin (Bacillariophyceae) under the conditions of cumulative cultivation

2020 ◽  
Vol 5 (1) ◽  
pp. 12-19
Author(s):  
R. G. Gevorgiz ◽  
S. N. Zheleznova
Keyword(s):  
Co2 Fixation ◽  
Carbon Fixation ◽  
Utilization Efficiency ◽  
Chlorella Protothecoides ◽  
Carbonic Anhydrases ◽  
Low Carbon ◽  
Carbon Utilization ◽  
Porphyridium Purpureum ◽  
Cylindrotheca Closterium ◽  
Dry Biomass

The carbon utilization efficiency is an important characteristic of the cultivated object. Diatom Cylindrotheca closterium (Ehrenberg) Reimann & J. C. Lewin is known to use carbon from aquatic environment quite effectively, as it has many unique carbonic anhydrases and carbon transporters. However, the carbon fixation efficiency for many types of diatoms in culture is still unknown. When calculating the carbon fixation efficiency, researchers use different terminology and methods, and it leads to significant difficulties when comparing the carbon fixation efficiency in the biomass of various types of microalgae. The aims of this study are: 1) to update terms and definitions used in literature on the basis of modern concepts of carbon fixation in microalgae biomass, as well as absorption of inorganic carbon by microalgae culture; 2) to evaluate the carbon fixation efficiency in the biomass of C. closterium diatom under conditions of cumulative cultivation. C. closterium was grown at a temperature of +20 °C on a nutrient medium RS. During the cultivation, the culture was bubbled with air (1.1 L of air per 1 L of culture per minute). The air temperature at the outlet of the suspension was of +19 °C; the maximum productivity of the culture was of 1.254 g·L−1·day−1. According to the results of the CHN analysis, the proportion of carbon in C. closterium dry biomass was of 23 %. Under the conditions of cumulative cultivation in C. closterium, the carbon fixation efficiency in biomass was of 90 %. Compared with other algae species, C. closterium is characterized by a rather high CO2 fixation efficiency. For example, in green microalga Chlorella protothecoides and Ch. vulgaris, the CO2 fixation efficiency was of 20 % and 55.3 %, respectively; in cyanobacteria Spirulina sp. – of 38 %; in red microalgae Porphyridium purpureum – of 69 %. It was observed that to ensure an increase of 1 g of C. closterium dry biomass per day at a temperature of +19 °C, a minimum of 0.46 L of CO2, or 1132 L of air, should be consumed. Possibly, it is high carbon fixation efficiency, as well as low carbon fraction in C. closterium biomass, that explains the high production indices of this species. Under equal conditions of cultivation in terms of light and carbon availability, the productivity of C. closterium can exceed the productivity of other types of microalgae by 5–10 times. So, while Spirulina sp. productivity reaches 0.2 g·L−1·day−1, C. closterium productivity is of 1.254 g·L−1·day−1.

scholarly journals FIRST RECORD OF Reimerothrix floridensis (FRAGILARIACEAE: BACILLARIOPHYTA) FOR MÉXICO

2014 ◽  
Vol 29 (1) ◽  
pp. 29
Author(s):  
O. U. Hernández -Almeida ◽  
J. A. Herrera -Silva
Keyword(s):  
First Record ◽  
Cylindrotheca Closterium

Primer registro de Reimerothrix floridensis (Fragilariaceae: Bacillariophyta) para México Reimerothrix floridensis forma parte de un grupo de diatomeas con forma arqueada que generalmente son confundidas con Cylindrotheca closterium, Psammosynedra closterioides y Nitzschia longissima var. reversa. En este trabajo se presenta el primer registro de la diatomea arqueada Reimerothrix floridensis , recolectada en Dzilam de Bravo (costa norte de la Península de Yucatán) en el extremo sur del Golfo de México. Este hallazgo se realizó en el marco de la identificación de las especies de diatomeas que conformaron una proliferación de fitoplancton suscitada en las costas de la Península de Yucatán durante 2011, y en donde C. closterium y N. longissima var. reversa fueron especies dominantes. Las características de Reimerothrix floridensis coinciden con la descripción original de la especie, excepto por las areolas asociadas al sternum. Dado que esta especie solo había sido registrada en la Bahía de Florida, su observación en México en la costa norte de la Península de Yucatán representa su registro más sureño, lo cual extiende el rango geográfico de esta especie. Por otra parte, debido a que la observación de Reimerothrix floridensis se realizó durante la presencia de una proliferación de C. closterium y N. longissima, especies con las que generalmente es confundida, se pone de manifiesto la importancia de realizar determinaciones taxonómicas cuidadosas acompañadas de descripciones detalladas que den certeza a los estudios ecológicos

scholarly journals Going with the Flow: Detection of Drift in Response to Hypo-Saline Stress by the Estuarine Benthic Diatom Cylindrotheca closterium

PLoS ONE ◽  
2013 ◽  
Vol 8 (11) ◽  
pp. e81073 ◽  
Author(s):  
Cristiano V. M. Araújo ◽  
Sonia Romero-Romero ◽  
Lucio F. Lourençato ◽  
Ignacio Moreno-Garrido ◽  
Julián Blasco ◽  
...  
Keyword(s):  
Benthic Diatom ◽  
Saline Stress ◽  
Cylindrotheca Closterium ◽  
Flow Detection

Photosynthetic rate of the marine diatom Cylindrotheca closterium

1967 ◽  
Vol 18 (2) ◽  
pp. 123 ◽  
Author(s):  
GF Humphrey ◽  
DV Subba Rao
Keyword(s):  
Blue Light ◽  
Photosynthetic Rate ◽  
White Light ◽  
Culture Medium ◽  
Spectral Composition ◽  
Compensation Point ◽  
Marine Diatom ◽  
Ph Change ◽  
Cylindrotheca Closterium

Photosynthesis in Cylindrotheca closterium was greatest in 1-day-old cells (2-4 � O2 per 106 cells per hour), declining to about 50% of this at 3 days and to about 10 % at 14 days. Due mainly to a decrease in the intensity of photosynthesis the ratio of photosynthesis to respiration fell from 6-8 at 1 day to 34 at 3 days, and to 0.6-1.6 at 14 days. The decline in photosynthesis started before phosphate or nitrate in the culture medium was exhausted, but was accompanied by a pH change from 7.4 before inoculation to 8.4 at 1 day and 9.2 at 3 days and thereafter. White light of 1000-1500f.c. (6.4-9.6 mW/cm2) saturated photosynthesis, the compensation point varying from 50 to 100 f.c. (0.3-0.6 mW/cm2). In blue light similar in spectral composition to that found at 10m below the surface of the ocean, the compensation point was 0.5-0.6 mW/cm2.

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