Correlation analysis for non-invasive quantitative monitoring of biological activity of recombinant enzyme using green fluorescence protein in Escherichia coli under various culture environments

2007 ◽  
Vol 24 (1) ◽  
pp. 99-101 ◽  
Author(s):  
Hyo Jin Yoo ◽  
Jeong Hyun Seo ◽  
Dong Gyun Kang ◽  
Hyung Joon Cha
Keyword(s):  
Escherichia Coli ◽  
Biological Activity ◽  
Correlation Analysis ◽  
Green Fluorescence Protein ◽  
Recombinant Enzyme ◽  
Green Fluorescence ◽  
Non Invasive ◽  
Quantitative Monitoring ◽  
Fluorescence Protein

scholarly journals Allocation of gene products to daughter cells is determined by the age of the mother in single Escherichia coli cells

2020 ◽  
Vol 287 (1926) ◽  
pp. 20200569 ◽  
Author(s):  
Chao Shi ◽  
Lin Chao ◽  
Audrey Menegaz Proenca ◽  
Andrew Qiu ◽  
Jasper Chao ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Growth Rate ◽  
Green Fluorescence Protein ◽  
Gene Products ◽  
Green Fluorescence ◽  
E Coli ◽  
Daughter Cells ◽  
Escherichia Coli Cells ◽  
Fluorescence Protein ◽  
Asymmetric Partitioning

Gene expression and growth rate are highly stochastic in Escherichia coli . Some of the growth rate variations result from the deterministic and asymmetric partitioning of damage by the mother to its daughters. One daughter, denoted the old daughter, receives more damage, grows more slowly and ages. To determine if expressed gene products are also allocated asymmetrically, we compared the levels of expressed green fluorescence protein in growing daughters descending from the same mother. Our results show that old daughters were less fluorescent than new daughters. Moreover, old mothers, which were born as old daughters, produced daughters that were more asymmetric when compared to new mothers. Thus, variation in gene products in a clonal E. coli population also has a deterministic component. Because fluorescence levels and growth rates were positively correlated, the aging of old daughters appears to result from both the presence of both more damage and fewer expressed gene products.

scholarly journals Expansion of EasyClone-MarkerFree toolkit for Saccharomyces cerevisiae genome with new integration sites

2021 ◽  
Author(s):  
Mahsa Babaei ◽  
Luisa Sartori ◽  
Alexey Karpukhin ◽  
Dmitrii Abashkin ◽  
Elena Matrosova ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Recombinant Dna ◽  
Green Fluorescence Protein ◽  
Cellular Growth ◽  
Green Fluorescence ◽  
Biotechnological Production ◽  
Yeast Saccharomyces Cerevisiae ◽  
Integration Sites ◽  
Fluorescence Protein ◽  
Integration Efficiency

Abstract Biotechnological production requires genetically stable recombinant strains. To ensure genomic stability, recombinant DNA is commonly integrated into the genome of the host strain. Multiple genetic tools have been developed for genomic integration into baker's yeast Saccharomyces cerevisiae. Previously, we had developed a vector toolkit EasyClone-MarkerFree for stable integration into eleven sites on chromosomes X, XI, and XII of S. cerevisiae. The markerless integration was enabled by CRISPR-Cas9 system. In this study, we have expanded the kit with eight additional intergenic integration sites located on different chromosomes. The integration efficiency into the new sites was above 80%. The expression level of green fluorescence protein (gfp) for all eight sites was similar or above XI-2 site from the original EasyClone-MarkerFree toolkit. The cellular growth was not affected by the integration into any of the new eight locations. The eight-vector expansion kit is available from AddGene.

Establishment of an Embryotoxicity Assay with Green Fluorescence Protein-expressing Embryonic Cell-derived Cardiomyocytes

1999 ◽  
Vol 27 (3) ◽  
pp. 471-484 ◽  
Author(s):  
Susanne Bremer ◽  
Maaike Van Dooren ◽  
Martin Paparella ◽  
Eugen Kossolov ◽  
Bernd Fleischmann ◽  
...  
Keyword(s):  
Green Fluorescence Protein ◽  
Embryonic Cell ◽  
Green Fluorescence ◽  
Fluorescence Protein
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