scholarly journals In Situ Bioconversion of Coconut Oil via Coconut Solid State Fermentation by Geotrichum candidum ATCC 34614

2013 ◽  
Vol 7 (3) ◽  
pp. 784-794 ◽  
Author(s):  
Anahita Khoramnia ◽  
Afshin Ebrahimpour ◽  
Boon Kee Beh ◽  
Oi Ming Lai
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Coconut Oil ◽  
Geotrichum Candidum ◽  
In Situ Bioconversion

scholarly journals Isolation and characterization of three distinct forms of lipases from Candida rugosa produced in solid state fermentation

2001 ◽  
Vol 44 (2) ◽  
pp. 213-221 ◽  
Author(s):  
Sailas Benjamin ◽  
Ashok Pandey
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Specific Activity ◽  
Candida Rugosa ◽  
Gel Filtration ◽  
Coconut Oil ◽  
Apparent Molecular Weight ◽  
Ammonium Sulphate Precipitation ◽  
Isolation And Characterization ◽  
Ultra Filtration

Three distinct forms (Lip A, Lip B and Lip C) of extra-cellular lipases (EC- 3.1.1.3), produced by Candida rugosa in solid state fermentation (SSF) were purified and characterised. SSF was carried out in glass columns using coconut oil cake and wheat bran. The enzyme was purified from the aqueous extract of fermented matter by ammonium sulphate precipitation, dialysis, ultra-filtration and gel filtration using Sephadex-200 to a 43-fold purification and 64.35-mg/ml specific activity. SDS-PAGE of purified enzyme revealed three distinct bands, indicating the existence of three iso-forms, Lip A, Lip B and Lip C with apparent molecular weight about 64,000, 62,000 and 60,000 Da, respectively. All the three iso-forms were optimally active at 35-40°C and pH 7-8. They showed marked differences in their Km values with different saturated and unsaturated triacyl glycerols. Ag++ and Hg++ strongly inhibited enzyme activity of all the iso-forms, Mn++ has no effect and Ca++ and Mg++ enhanced the activity. EDTA also strongly inhibited the enzyme activities of iso-forms. However, activities of all the three lipases were completely inhibited by serine protease inhibitors such as 3,4-dichloroisocoumarin, pefabloc and partially by phenylmethanesulphonyl fluoride. To the best of our knowledge, this is the first report describing the purification and characterisation of C. rugosa lipase iso-forms from solid cultures. These lipase iso-forms with diverse characteristics produced in solid cultures may find potential application in biomedical field.

scholarly journals Improvement of Medium Chain Fatty Acid Content and Antimicrobial Activity of Coconut Oil via Solid-State Fermentation Using a MalaysianGeotrichum candidum

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Anahita Khoramnia ◽  
Afshin Ebrahimpour ◽  
Raheleh Ghanbari ◽  
Zahra Ajdari ◽  
Oi-Ming Lai
Keyword(s):  
Solid State ◽  
Lauric Acid ◽  
Fatty Acid Content ◽  
Coconut Oil ◽  
Value Added ◽  
Geotrichum Candidum ◽  
Antibacterial Activities ◽  
Medium Chain ◽  
Solid Media ◽  
Pharmaceutical Industries

Coconut oil is a rich source of beneficial medium chain fatty acids (MCFAs) particularly lauric acid. In this study, the oil was modified into a value-added product using direct modification of substrate through fermentation (DIMOSFER) method. A coconut-based and coconut-oil-added solid-state cultivation using a Malaysian lipolyticGeotrichum candidumwas used to convert the coconut oil into MCFAs-rich oil. Chemical characteristics of the modified coconut oils (MCOs) considering total medium chain glyceride esters were compared to those of the normal coconut oil using ELSD-RP-HPLC. Optimum amount of coconut oil hydrolysis was achieved at 29% moisture content and 10.14% oil content after 9 days of incubation, where the quantitative amounts of the modified coconut oil and MCFA were 0.330 mL/g of solid media (76.5% bioconversion) and 0.175 mL/g of solid media (53% of the MCO), respectively. MCOs demonstrated improved antibacterial activity mostly due to the presence of free lauric acid. The highest MCFAs-rich coconut oil revealed as much as 90% and 80% antibacterial activities againstStaphylococcus aureusandEscherichia coli, respectively. The results of the study showed that DIMOSFER by a local lipolyticG. candidumcan be used to produce MCFAs as natural, effective, and safe antimicrobial agent. The produced MCOs and MCFAs could be further applied in food and pharmaceutical industries.

In situ bioremediation using biosurfactant produced by solid state fermentation

2009 ◽  
Vol 25 (5) ◽  
pp. 843-851 ◽  
Author(s):  
Vilásia Guimarães Martins ◽  
Susana Juliano Kalil ◽  
Jorge Alberto Vieira Costa
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
In Situ Bioremediation

Aroma compounds production by solid state fermentation, importance of in situ gas-phase recovery systems

2018 ◽  
Vol 102 (17) ◽  
pp. 7239-7255 ◽  
Author(s):  
Sophal Try ◽  
Andrée Voilley ◽  
Thavarith Chunhieng ◽  
Joëlle De-Coninck ◽  
Yves Waché
Keyword(s):  
Solid State ◽  
Gas Phase ◽  
Solid State Fermentation ◽  
Aroma Compounds ◽  
Phase Recovery

scholarly journals Isolation and characterization of three distinct forms of lipases from Candida rugosa produced in solid state fermentation

2000 ◽  
Vol 43 (5) ◽  
pp. 453-460 ◽  
Author(s):  
Sailas Benjamin ◽  
Ashok Pandey
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Specific Activity ◽  
Candida Rugosa ◽  
Gel Filtration ◽  
Coconut Oil ◽  
Apparent Molecular Weight ◽  
Ammonium Sulphate Precipitation ◽  
Isolation And Characterization ◽  
Ultra Filtration

Three distinct forms (Lip A, Lip B and Lip C) of extra-cellular lipases (EC- 3.1.1.3), produced by Candida rugosa in solid state fermentation (SSF) were purified and characterised. SSF was carried out in glass columns using coconut oil cake and wheat bran. The enzyme was purified from the aqueous extract of fermented matter by ammonium sulphate precipitation, dialysis, ultra-filtration and gel filtration using Sephadex-200 to a 43-fold purification and 64.35-mg/ml specific activity. SDS-PAGE of purified enzyme revealed three distinct bands, indicating the existence of three iso-forms, Lip A, Lip B and Lip C with apparent molecular weight about 64,000, 62,000 and 60,000 Da, respectively. All the three iso-forms were optimally active at 35-40ºC and pH 7-8. They showed marked differences in their Km values with different saturated and unsaturated triacyl glycerols. Ag++ and Hg++ strongly inhibited enzyme activity of all the iso-forms, Mn++ has no effect and Ca++ and Mg++ enhanced the activity. EDTA also strongly inhibited the enzyme activities of iso-forms. However, activities of all the three lipases were completely inhibited by serine protease inhibitors such as 3,4-dichloroisocoumarin, pefabloc and partially by phenylmethanesulphonyl fluoride. To the best of our knowledge, this is the first report describing the purification and characterisation of C. rugosa lipase iso-forms from solid cultures. These lipase iso-forms with diverse characteristics produced in solid cultures may find potential application in biomedical field.

scholarly journals Solid State Fermentation of Aspergillus oryzae for Glucoamylase Production on Agro residues

2010 ◽  
Vol 4 ◽  
pp. 16-25 ◽  
Author(s):  
Vasudeo Zambare
Keyword(s):  
Solid State ◽  
Aspergillus Oryzae ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Coconut Oil ◽  
Groundnut Oil ◽  
Amylolytic Enzyme ◽  
Coconut Oil Cake ◽  
Agro Residues ◽  
Glucoamylase Production

Glucoamylase is a well recognized amylolytic enzyme used in food industry, which is generally produced by Aspergillus genus under solid-state fermentation (SSF). This study presents production of glucoamylase by Aspergillus oryzae on the solid surface of rice husk, wheat bran, rice bran, cotton seed powder, corn steep solids, bagasse powder, coconut oil cake, and groundnut oil cake as substrates. Optimization of the SSF media and parameters resulted in a 24% increase in the glucoamylase activity. Optimum glucoamylase production (1986 μmoles of glucose produced per minute per gram of dry fermented substrate) was observed on wheat bran supplemented with 1%, (w/w) starch, 0.25%, (w/w) urea at pH 6, 100%, (v/w) initial moisture and 30°C after incubation 120 hrs. Therefore, A. oryzae can be useful in bioprocessing application for saccharification of agro-residues. Keywords: Glucoamylase, Aspergillus oryzae, solid state fermentation, agro residues DOI: 10.3126/ijls.v4i0.2892 International Journal of Life Sciences Vol.4 2010 pp.16-25

scholarly journals Lovastatin production by Aspergillus fischeri under solid state fermentation from coconut oil cake

1970 ◽  
Vol 2 (1) ◽  
pp. 26-36 ◽  
Author(s):  
Parvatham Madhu Latha ◽  
Pallem Chanakya ◽  
Manipati Srikanth
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Maximum Yield ◽  
Initial Moisture Content ◽  
Coconut Oil ◽  
Malt Extract ◽  
Fermentation Time ◽  
Aspergillus Fischeri ◽  
Fermentation Parameters ◽  
Coconut Oil Cake

The main aim of the present investigation was to optimize the fermentation parameters that enhance the maximum production of lovastatin by Aspergillus fischeri using coconut oil cake as the solid substrate under solid state fermentation. The maximum yield of lovastatin (14.77 mg/g dry substrate) using coconut oil cake as the substrate was achieved with the following optimized process parameters: fermentation time (7 days), initial moisture content (60% v/w), inoculum volume (2ml of five day old culture), initial pH (5.0), incubation temperature (30ºC), lactose (1% w/v) and malt extract (1% w/v).Keywords: Lovastatin; Aspergillus fischeri; Coconut oil cake; Fermentation parameters; OptimizationDOI: http://dx.doi.org/10.3126/njb.v2i1.5641 Nepal Journal of Biotechnology Jan.2012, Vol.2(1): 26-36 

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