Isolation and Identification of Two New Fungal Strains for Xylanase Production

2010 ◽  
Vol 162 (6) ◽  
pp. 1626-1634 ◽  
Author(s):  
Yasser Bakri ◽  
Magali Masson ◽  
Philippe Thonart
2005 ◽  
Vol 69 (1) ◽  
pp. 71-78 ◽  
Author(s):  
Zawadi A. Chipeta ◽  
James C. du Preez ◽  
George Szakacs ◽  
Lew Christopher

2012 ◽  
Vol 19 ◽  
pp. 7-14
Author(s):  
SCD Sharma ◽  
MS Shovon ◽  
AKM Asaduzzaman ◽  
MG Sarowar Jahan ◽  
T Yeasmin ◽  
...  

Context: To analyze the nutritional and physicochemical parameters for the production of alkali-thermostable and cellulase free xylanase from bacteria. Objectives: The aim of this study was to isolation and identification and of alkali-thermostable and cellulase free xylanase producing bacteria from soil as well as optimization of process parameters for xylanase production. Materials and Methods: The bacterium Bacillus sp. was isolated from soil by serial dilution technique on xylan agar medium and identified by morphological and biochemical studies. The production of xylanase was carried out on xylan broth medium and xylanase activity was assayed by dinitrosalicylic acid (DNS) method. The effect of cultural parameters on the production of xylanase was determined by measuring the activity of xylanase. The effect of temperature and pH on the activity of partially purified xylanase as well as substrate specificity of xylanase were examined. Results: The maximum xylanase production (4000 U/L) by a Bacillus sp. was attained when the medium containing 0.5% wheat bran xylan and peptone at pH 8.0 and 50-55°C within 48-60 h. The partially purified xylanase was optimally active at pH 9.0 and 55°C. The xylanase showed high substrate activity towards wheat bran xylan but no activity towards cellulose, carboxymethyl cellulose and starch. Thus the enzyme was alkali-thermostable and cellulase free xylanase. Conclusion: The results obtained in this study suggest that the Bacillus sp. used is highly potential and useful for the production of cellulase free xylanase. DOI: http://dx.doi.org/10.3329/jbs.v19i0.12994 J. bio-sci. 19: 7-14, 2011


2018 ◽  
Vol 15 (4) ◽  
pp. 763-770
Author(s):  
Pham Thi Thu Hang ◽  
Le Thi Quynh Tram ◽  
Tran Phuong Anh ◽  
Ho To Thi Khai Mui ◽  
Dang Nguyen Thao Vi ◽  
...  

Organic waste is gradually degraded during composting process, producing carbon dioxide, water, heat, and humus, the relatively stable end product. The degradation process is carried out by living organisms, of which fungi appear to have the most important role since they break down tough debris (cellulose, lignin, and other resistant materials), enabling other microorganisms to continue the decomposition process. The objective of this study was to isolate and identify the fungi associated with large scale municipal biosolid waste composting process in Vietnam. In this study, we have isolated 10 morphologically different fungal strains from the composting materials, and classified based on morphological characteristics and 18S rDNA sequences. The results showed that these fungal strains belonged to four different genera, including Aspergillus, Penicillium, Monascus, and Trichoderma. The results would be a useful reference for further studies of diversity, and functions of fungi that involved in municipal biosolid waste composting process in Vietnam environmental conditions.


1994 ◽  
Vol 49 (1) ◽  
pp. 13-16 ◽  
Author(s):  
Anna Wia̧cek-Żychlińska ◽  
Joanna Czakaj ◽  
Regina Sawicka-Żukowska

2017 ◽  
Vol 21 (1) ◽  
pp. 1-6
Author(s):  
Vellaichamy Mageshwaran ◽  
Sharmila Majee ◽  
Kuppusamy Pandiyan

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