<i>Mineral and nitrogen source amendment for the cellulase and xylanase production by different fungal strains using DDGS as the feedstock</i>

2021 ◽  
Author(s):  
Attia Iram ◽  
Deniz Cekmecelioglu ◽  
Ali Demirci
Keyword(s):  
Nitrogen Source ◽  
Xylanase Production ◽  
Fungal Strains

Isolation and Identification of Two New Fungal Strains for Xylanase Production

2010 ◽  
Vol 162 (6) ◽  
pp. 1626-1634 ◽  
Author(s):  
Yasser Bakri ◽  
Magali Masson ◽  
Philippe Thonart
Keyword(s):  
Isolation And Identification ◽  
Xylanase Production ◽  
Fungal Strains

scholarly journals Production and Partial Characterization of an Alkaline Xylanase from a Novel FungusCladosporium oxysporum

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Guo-Qiang Guan ◽  
Peng-Xiang Zhao ◽  
Jin Zhao ◽  
Mei-Juan Wang ◽  
Shu-Hao Huo ◽  
...  
Keyword(s):  
Nitrogen Source ◽  
Wheat Bran ◽  
Internal Transcribed Spacer ◽  
Gene Sequence ◽  
Agricultural Waste ◽  
Xylanase Activity ◽  
Maximum Activity ◽  
Alkaline Proteases ◽  
Xylanase Production

A new fungusCladosporium oxysporumGQ-3 producing extracellular xylanase was isolated from decaying agricultural waste and identified based on the morphology and comparison of internal transcribed spacer (ITS) rDNA gene sequence.C. oxysporumproduced maximum xylanase activity of 55.92 U/mL with wheat bran as a substrate and NH4Cl as a nitrogen source. Mg2+improvedC. oxysporumxylanase production.Partially purified xylanase exhibited maximum activity at 50°C and pH 8.0, respectively, and showed the stable activity after 2-h treatment in pH 7.0–8.5 or below 55°C. Mg2+enhanced the xylanase activity by 2% while Cu2+had the highest inhibition ratio of 57.9%. Furthermore,C. oxysporumxylanase was resistant to most of tested neutral and alkaline proteases. Our findings indicated thatCladosporium oxysporumGQ-3 was a novel xylanase producer, which could be used in the textile processes or paper/feed industries.

Xylanase production by fungal strains on spent sulphite liquor

2005 ◽  
Vol 69 (1) ◽  
pp. 71-78 ◽  
Author(s):  
Zawadi A. Chipeta ◽  
James C. du Preez ◽  
George Szakacs ◽  
Lew Christopher
Keyword(s):  
Xylanase Production ◽  
Fungal Strains ◽  
Spent Sulphite Liquor

Optimization of Xylanase Production by Streptomyces costaricanus 45I-3 Using Various Substrates through Submerged Fermentation

2020 ◽  
Vol 14 (1) ◽  
pp. 5
Author(s):  
SIPRIYADI SIPRIYADI ◽  
ARIS TRI WAHYUDI ◽  
MAGGY THENAWIDJAYA SUHARTONO ◽  
ANJA MERYANDINI
Keyword(s):  
Nitrogen Source ◽  
Yeast Extract ◽  
Xylanase Activity ◽  
Hydrolytic Enzymes ◽  
Tween 80 ◽  
Medium Composition ◽  
Production Costs ◽  
Xylanase Production ◽  
Beechwood Xylan

Xylanase is an important hydrolytic enzymes with many application in several industries, but to obtain enzyme derived products is not easy. Thus, the optimization of efficient xylanases production is a great interest for biotechnological application. This study aims to determine the type of substrate, medium composition, and optimum conditions of xylanase production by S. costaricanus 45I-3. Determination of substrate type was done by growing the tested bacteria on birchwood xylan, beechwood xylan, oat spelled xylan, corn cobs xylan, and tobacco xylan substrate, meanwhile the determination of medium composition and enzyme production were done by measuring xylanase activity at various substrate concentration and replacing the carbon, nitrogen, phosphate and surfactants source. The results showed that the highest enzymatic index (EI) produced from corn cob xylan substrate at 3.60 meanwhile the second highest was beechwood xylan substrate at 2.87 EI, however this substrate is purer, thus this substrate was selected and used as xylan sources for further optimization measurement. The best xylanase activity (2.29 U/mL) obtained on eighth day after inoculation on rotary incubator at 120 rpm in 28 ºC. Arabinose as the source of carbon generate the highest activity at 3.161 U/mL meanwhile the most preferred source of phosphate is Na2HPO4 (2.37 U/mL). Both source of nitrogen i.e. nitrogen ammonium sulphate (NH4)2SO4 and yeast extract were able to produce xylanase at 2.57 and 2.36 U/mL. The addition of surfactant in production medium showed addition of SDS surfactant (0.146 U/mL) and Tween 80 (0.438 U/mL) showed a negative response by decreasing the activity. The conclusion showed that the xylanase activity was increased after optimization at various C, N, and P sources, and the use of nitrogen source (NH4)2SO4), become a more economical alternative to replacing a nitrogen source yeast extract so it can lower the production costs of xylanase enzyme.

Xylanase production by fungal strains in solid-state fermentations

1994 ◽  
Vol 49 (1) ◽  
pp. 13-16 ◽  
Author(s):  
Anna Wia̧cek-Żychlińska ◽  
Joanna Czakaj ◽  
Regina Sawicka-Żukowska
Keyword(s):  
Solid State ◽  
Xylanase Production ◽  
Fungal Strains

scholarly journals Screening of Culture Conditions for Production of Xylanase from Landfill Soil Bacteria

2019 ◽  
Vol 19 (2) ◽  
pp. 470 ◽  
Author(s):  
Siti Nor Amira Rosli ◽  
Rohaida Che Man ◽  
Nasratun Masngut
Keyword(s):  
Carbon Source ◽  
Moisture Content ◽  
Nitrogen Source ◽  
Incubation Period ◽  
Xylanase Activity ◽  
Nitrogen Sources ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Xylanase Production ◽  
Initial Ph

Culture conditions including initial pH media, incubation period, inoculum size, type of carbon source, type of nitrogen source and its concentration, which affect xylanase production were screened via the one-factor-at-a-time approach. The bacteria used in the production of xylanase was isolated from the landfill site at Sg. Ikan, Kuala Terengganu, Malaysia. Three characterizations of the landfill soil were investigated for their moisture content, ash content, and pH. The culture conditions range used in the experimental work were between 6–30 h for the incubation period, with initial pH between 5–9, inoculum size between 1–20% v/v, carbon, nitrogen sources, and nitrogen source concentration between 1–5% w/v. Xylanase activity was estimated using dinitrosalicylic acid (DNS) based on the release of xylose under standard assay conditions. The landfill soil was observed to have pH between pH 3.4–7.2 with a moisture content between 12.4–33.7% and ash ranged between 3.5–4.3%. Results showed that the highest xylanase activity within studied ranges was recorded at 25.91±0.0641 U/mL with 10% (v/v) inoculum size, 1% (w/v) xylose as sole carbon source, mixture of 1% (w/v) peptone and 0.25% (w/v) ammonium sulphate as nitrogen sources, which was carried out at initial pH of 8.0 for 24 h incubation.

In vitro Evaluation of Ammonium Base Sulfite Liquor as an Energy and Nitrogen Source for Ruminants

1976 ◽  
Vol 42 (4) ◽  
pp. 993-1001 ◽  
Author(s):  
R. P. Kromann ◽  
T. R. Wilson ◽  
G. S. Cantwell
Keyword(s):  
Nitrogen Source ◽  
In Vitro Evaluation
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