Microbial Community Analysis of Digested Liquids Exhibiting Different Methane Production Potential in Methane Fermentation of Swine Feces

2020 ◽  
Vol 191 (3) ◽  
pp. 1140-1154 ◽  
Author(s):  
Yoshitaka Nakamura ◽  
Matsujiro Ishibashi ◽  
Yoshinori Kamitani ◽  
Hirohito Tsurumaru
Keyword(s):  
Microbial Community ◽  
Methane Production ◽  
Community Analysis ◽  
Microbial Community Analysis ◽  
Production Potential ◽  
Methane Fermentation ◽  
Swine Feces ◽  
Methane Production Potential

scholarly journals Microbial Community Analysis Using MiSeq Sequencing and Pathway of Methane Production in Tehran WWTP: A Full-Scale Anaerobic Digester

2020 ◽  
Author(s):  
Reza Barati Rashvanlou ◽  
Mahdi Farzadkia ◽  
Abbas Ali Moserzadeh ◽  
Asghar Riazati ◽  
Chiang Wei ◽  
...  
Keyword(s):  
Microbial Community ◽  
Methane Production ◽  
Community Analysis ◽  
Full Scale ◽  
Microbial Community Analysis ◽  
Miseq Sequencing ◽  
Sequencing Method ◽  
Community Data ◽  
Rank Abundance ◽  
Made In

Introduction: One of biological wastewater treatment methods that utilizes to both digesting waste activated sludge and methane production is anaerobic digestion (AD). It is believed to be most effective solution in terms of energy crisis and environmental pollution issues. Materials and Methods: In this study the sludge was digested anaerobically sampled from a full-scale WWTP, located at south of Tehran, Iran for evaluation. To study the microbial community within the sludge the MiSeq Sequencing method utilized. Based on our field data (data not shown) and microbial community data, a schematic diagram of probable leading pathways was made in the studied digester. Results: At first, the community variety in the bulk sludge and richness were enhanced followed by loading increasing. Meanwhile, the loading change enhanced the community richness and variety of the sludge. By comparing the rank-abundance distributions, a shallow gradient would show high evenness since the abundances of diverse species are alike. The results showed all the communities were extremely diverse and 15 phyla were distinguished in the sludge sample. The dominant phyla of the community were Bacteroidetes and Firmicutes and quantity of the two phyla were 21% and 11%, respectively. Anaerobaculum, Acinetobacter, Syntrophomonas, and Coprothermobacter were the chief genera for the microbial communities and the sum of four genera were 7%, 3%, 3%, and 2%, respectively. Conclusion: It was shown that syntrophic acetate oxidizing bacterias (SAOBs) metabolized acetate through hydrogen trophic methanogenesis in the digester. Generally, the findings may be useful to help the wastewater operators to utilize an effective method that able to treat waste sludge plus methane production, simultaneously.

Methane production and microbial community analysis in the goethite facilitated anaerobic reactors using algal biomass

Fuel ◽  
2015 ◽  
Vol 145 ◽  
pp. 196-201 ◽  
Author(s):  
Juan Tan ◽  
Jin Wang ◽  
Jun Xue ◽  
Shao-Yang Liu ◽  
Shu-Chuan Peng ◽  
...  
Keyword(s):  
Microbial Community ◽  
Methane Production ◽  
Algal Biomass ◽  
Community Analysis ◽  
Microbial Community Analysis ◽  
Anaerobic Reactors

scholarly journals Co-digestion of hybrid Pennisetum and peanut shell after adding TiO2 nanoparticles: Focusing on the synergistic effects on methane production

2021 ◽  
Author(s):  
HongMei Zhao ◽  
Meng Jiang
Keyword(s):  
Microbial Community ◽  
Anaerobic Digestion ◽  
Methane Production ◽  
Synergistic Effects ◽  
Community Analysis ◽  
Gas Production ◽  
Microbial Community Analysis ◽  
Peanut Shell ◽  
Hybrid Pennisetum ◽  
Mesophilic Conditions

Abstract Anaerobic digestion is a widely accepted method to treat wastes such as peanut shell. The energy and nutrients are simultaneously recovered by this method. The objective of this study was to elucidate the effect of TiO 2 nanoparticles in co-digestion of hybrid Pennisetum and peanut shell under mesophilic conditions. The results demonstrated the methane (CH 4 ) production was improved by adding the TiO 2 nanoparticles. The cumulative gas production is best (up to 11,133.3 mL) by adding 0.15% nano-TiO 2 particles. The microbial community analysis showed that Methanobacterium and Methanosarcina were enriched in the presence of TiO 2 nanoparticles indicating that TiO 2 can improve CH 4 production by stimulating the growth of methanogens.

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