Antimicrobial agents and microbial ecology

<abstract> <p>Antimicrobials are therapeutic substances used to prevent or treat infections. Disinfectants are antimicrobial agents applied to non-living surfaces. Every year, several thousand tonnes of antimicrobials and their by-products are released into the environment and in particular into the aquatic environment. This type of xenobiotic has ecological consequences in the natural environment but also in technological environments such as wastewater treatment plants and methane fermentation sewage sludge treatment plants. The constant exposure of microbial communities not only to high concentrations but also to sub-inhibitory concentrations of antibiotics is a key element in the development of antibiotic resistance in aquatic environments and in soils. The future of antimicrobials lies in the development of biosourced or bioinspired molecules. The observation and deciphering of interactions between living organisms is the key to this development.</p> </abstract>

Conversion of Carbohydrates in Lignocellulosic Biomass after Chemical Pretreatment

The aim of the study was to determine the quantitative and qualitative changes taking place in biomass components actively participating in methane fermentation, i.e., in carbohydrates, as a result of chemical pretreatment. Analyses were conducted on agricultural waste (corn stover, also called corn straw, and corncobs) as materials most commonly used in methane fermentation, as well as poplar wood, a material relatively rarely used in biogas production. Pretreatment with the aim of increasing efficiency of methane fermentation was carried out with the use of acid and alkaline solutions of different concentrations. The effect of pretreatment on carbohydrates was analyzed based on the quantitative and qualitative changes in this component. Due to the structural heterogeneity of carbohydrates, their varied reactivity and fermentability were determined in terms of holocellulose, cellulose, and pentosans. The chemical structure of cellulose was also analyzed. It is shown in this study that chemical pretreatment causes transformations of carbohydrate components, which differ quantitatively and qualitatively in the compared raw materials. It was found that the alkaline treatment caused smaller changes in the percentage shares of the carbohydrate biomass components as compared to the acid treatment. Moreover, it was observed that the compared materials differ in terms of quantitative changes in their chemical composition depending on the composition of the raw material prior to pretreatment. In the case of corn waste subjected to the action of 1 and 3% NaOH, the share of pentosans in the biomass increased. It was established that this is a change with a positive effect on fermentation efficiency. The action of acids and alkalis on the biomass led to similar structural changes in cellulose, which are adverse for the fermentation process.

The Effect of Electromagnetic Microwave Radiation on Methane Fermentation of Selected Energy Crop Species

The aim of the present study was to determine how thermal stimulation via electromagnetic microwave radiation impacts the yields of biogas and methane produced by methane fermentation of five selected energy crop species in anaerobic reactors. The resultant performance was compared with that of reactors with conventional temperature control. The highest biogas production capacity was achieved for maize silage and Virginia mallow silage (i.e., 680 ± 28 dm3N/kgVS and 506 ± 16 dm3N/kgVS, respectively). Microwave radiation as a method of heating anaerobic reactors provided a statistically-significantly boost in methane production from maize silage (18% increase). Biomethane production from maize silage rose from 361 ± 12 dm3N/kgVS to 426 ± 14 dm3N/kgVS. In the other experimental variants, the differences between methane concentrations in the biogas were non-significant.

Increasing the Biogas Potential of Rapeseed Straw Using Pulsed Electric Field Pre-Treatment

Due to the high availability of lignocellulosic biomass, which can be obtained from terrestrial plants, agricultural waste biomass, and the agro-food, paper or wood industries, its use for energy production by methane fermentation is economically and environmentally justified. However, due to their complex structures, lignocellulosic substrates have a low conversion factor to biogas. Therefore, scientists are still working on the development of new methods of the pre-treatment of lignocellulosic materials that will increase the biogas productivity from lignocellulosic biomass. The presented research focuses on the use of a pulsed electric field (PEF) to disintegrate rapeseed straw prior to the methane fermentation process. Scanning electron microscopy observation showed that, in the disintegrated sample, the extent of damage to the plant tissue was more severe than in the control sample. In the sample disintegrated for 7 min, the chemical oxygen demand increased from 4146 ± 75 mg/L to 4920 ± 60 mg/L. The best result was achieved with a 5-min PEF pre-treatment. The methane production reached 290.8 ± 12.1 NmL CH4/g VS, and the biogas production was 478.0 ± 27.5 NmL/g VS; it was 14% and 15% higher, respectively, compared to the control sample.

Methane Fermentation Residue Compost Derived from Food Waste to Aid Komatsuna (Brassica rapa) Growth

One suitable solution to achieve sustainable development goals (SDGs) is to utilize methane fermentation residue obtained from food waste. However, methane fermentation residue compost is often difficult to use due to its inhibitory properties and pungent smell. To evaluate chemical parameters and plant growth, we examined the use of methane-fermented food waste residue compost (FWM), three types of animal manure, namely, horse manure (HM), cow manure (CWM), and chicken manure (CKM). Our results revealed that mixing food waste compost with cow and horse manure reduced the inhibition of Komatsuna germination, however, chicken manure blending limited inhibition reduction. The improvement of GI in the combination of FWM and animal manure was obtained at a ratio 1:4 with an improvement in GI of 20.8%, 16.8%, and 3.8% for combination FWM + HM, FWM + CWM, and FWM + CKM, respectively. The ratio of the combination of FWM + HM could be increased to a ratio of 2:3 with an improvement of 14.7%. Additionally, for Komatsuna growth, FWM with cow and horse manure mixer considerably enhanced plant growth and yield. Furthermore, the combination of FWM + HM and FWM + CWM could improve the ratio of NUE compost/NUE FWM with the values being 12.0 and 11.1, respectively. Therefore, combining FWM with CWM and HM increases the utility value of methane fermentation residues obtained from food waste as compost for maintaining soil fertility, while reducing the use of chemical fertilizer.

Progress in the Production of Biogas from Maize Silage after Acid-Heat Pretreatment

One of the most effective technologies involving the use of lignocellulosic biomass is the production of biofuels, including methane-rich biogas. In order to increase the amount of gas produced, it is necessary to optimize the fermentation process, for example, by substrate pretreatment. The present study aimed to analyze the coupled effects of microwave radiation and the following acids: phosphoric(V) acid (H3PO4), hydrochloric acid (HCl), and sulfuric(VI) acid (H2SO4), on the destruction of a lignocellulosic complex of maize silage biomass and its susceptibility to anaerobic degradation in the methane fermentation process. The study compared the effects of plant biomass (maize silage) disintegration using microwave and conventional heating; the criterion differentiating experimental variants was the dose of acid used, i.e., 10% H3PO4, 10% HCl, and 10% H2SO4 in doses of 0.02, 0.05, 0.10, 0.20, and 0.40 g/gTS. Microwave heating caused a higher biogas production in the case of all acids tested (HCl, H2SO4, H3PO4). The highest biogas volume, exceeding 1800 L/kgVS, was produced in the variant with HCl used at a dose of 0.4 g/gTS.

Study of the significance of the ph of the substrate of the biogas installation during treating it with a low-frequency electromagnetic field

The article considers the issue of studying the value of pH, substrate in the process of methane fermentation in the mesophilic regime and the influence of the electromagnetic field of industrial frequency. The aim is to investigate the influence of electromagnetic fields on the pH value of the substrate during fermentation. Different types of microorganisms are involved in the process of methanogenesis, and the decisive role in it is played by methane-forming archaea, which are most sensitive to pH and should be in the range of 6.5 - 8. Therefore, it is necessary to check the effect of low frequency electromagnetic field on substrate pH. The study was performed for 25 days on two substrates, one of which was exposed to a low-frequency electromagnetic field with an electromagnetic induction of 3.5 mT. The research results show that the pH value of the substrate exposed to the electromagnetic field during the methane fermentation process was within acceptable limits, and the second substrate decreased, that is, it was acidified. Key words: methane fermentation, substrate, pH value, electromagnetic field

Odour Nuisance at Municipal Waste Biogas Plants and the Effect of Feedstock Modification on the Circular Economy—A Review

The increase in the amount of municipal solid waste (MSW) generated, among other places, in households is a result of the growing population, economic development, as well as the urbanisation of areas with accompanying insufficiently effective measures to minimise waste generation. There are many methods for treating municipal waste, with the common goal of minimising environmental degradation and maximising resource recovery. Biodegradable waste, including selectively collected biowaste (BW), also plays an essential role in the concept of the circular economy (CE), which maximises the proportion of waste that can be returned to the system through organic recycling and energy recovery. Methane fermentation is a waste treatment process that is an excellent fit for the CE, both technically, economically, and environmentally. This study aims to analyse and evaluate the problem of odour nuisance in municipal waste biogas plants (MWBPs) and the impact of the feedstock (organic fraction of MSW-OFMSW and BW) on this nuisance in the context of CE assumptions. A literature review on the subject was carried out, including the results of our own studies, showing the odour nuisance and emissions from MWBPs processing both mixed MSW and selectively collected BW. The odour nuisance of MWBPs varies greatly. Odour problems should be considered regarding particular stages of the technological line. They are especially seen at the stages of waste storage, fermentation preparation, and digestate dewatering. At examined Polish MWBPs cod ranged from 4 to 78 ou/m3 for fermentation preparation and from 8 to 448 ou/m3 for digestate dewatering. The conclusions drawn from the literature review indicate both the difficulties and benefits that can be expected with the change in the operation of MWBPs because of the implementation of CE principles.