Cadmium Concentration in Human Autopsy Tissues

2017 ◽  
Vol 179 (2) ◽  
pp. 172-177 ◽  
Author(s):  
Teresa Lech ◽  
Józefa K. Sadlik
Keyword(s):  
Cadmium Concentration ◽  
Autopsy Tissues ◽  
Human Autopsy

SI08 Protein Misfolding Cyclic Amplification of Disease-Associated Prion Protein Using Human Autopsy Tissues

2006 ◽  
Vol 16 (s1) ◽  
pp. 7-7
Author(s):  
M. Jones ◽  
A. Peden ◽  
J. Ironside ◽  
C. Prowse ◽  
I. MacGregor ◽  
...  
Keyword(s):  
Prion Protein ◽  
Protein Misfolding ◽  
Protein Misfolding Cyclic Amplification ◽  
Autopsy Tissues ◽  
Human Autopsy

Detection of Lipid Peroxidation-Induced DNA Adducts Caused by 4-Oxo-2(E)-nonenal and 4-Oxo-2(E)-hexenal in Human Autopsy Tissues

2010 ◽  
Vol 23 (9) ◽  
pp. 1442-1448 ◽  
Author(s):  
Pei-Hsin Chou ◽  
Shinji Kageyama ◽  
Shun Matsuda ◽  
Keishi Kanemoto ◽  
Yoshiaki Sasada ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Dna Adducts ◽  
Autopsy Tissues ◽  
Human Autopsy

scholarly journals Assessing and improving the validity of COVID-19 autopsy studies - a multi center approach to establish essential standards for immunohistochemical and ultrastructural analyses

2022 ◽  
Author(s):  
Susanne Krasemann ◽  
Carsten Dittmayer ◽  
Saskia v. Stillfried ◽  
Jenny Meinhardt ◽  
Fabian Heinrich ◽  
...  
Keyword(s):  
Comprehensive Evaluation ◽  
Organ Damage ◽  
P Value ◽  
Structural Protein ◽  
Secondary Effects ◽  
Virus Identification ◽  
Qualitative And Quantitative ◽  
Inter Observer Variability ◽  
Autopsy Tissues ◽  
Human Autopsy

Background Autopsy studies have provided valuable insights into the pathophysiology of COVID-19. Controversies remain whether the clinical presentation is due to direct organ damage by SARS-CoV-2 or secondary effects, e.g. by an overshooting immune response. SARS-CoV-2 detection in tissues by RT-qPCR and immunohistochemistry (IHC) or electron microscopy (EM) can help answer these questions, but a comprehensive evaluation of these applications is missing. Methods We assessed publications using IHC and EM for SARS-CoV-2 detection in autopsy tissues. We systematically evaluated commercially available antibodies against the SARS-CoV-2 spike protein and nucleocapsid, dsRNA, and non-structural protein Nsp3 in cultured cell lines and COVID-19 autopsy tissues. In a multicenter study, we evaluated specificity, reproducibility, and inter-observer variability of SARS-CoV-2 nucleocapsid staining. We correlated RT-qPCR viral tissue loads with semiquantitative IHC scoring. We used qualitative and quantitative EM analyses to refine criteria for ultrastructural identification of SARS-CoV-2. Findings Publications show high variability in the detection and interpretation of SARS-CoV-2 abundance in autopsy tissues by IHC or EM. In our study, we show that IHC using antibodies against SARS-CoV-2 nucleocapsid yields the highest sensitivity and specificity. We found a positive correlation between presence of viral proteins by IHC and RT-qPCR-determined SARS-CoV-2 viral RNA load (r=-0.83, p-value <0.0001). For EM, we refined criteria for virus identification and also provide recommendations for optimized sampling and analysis. 116 of 122 publications misinterpret cellular structures as virus using EM or show only insufficient data. We provide publicly accessible digitized EM and IHC sections as a reference and for training purposes. Interpretation Since detection of SARS-CoV-2 in human autopsy tissues by IHC and EM is difficult and frequently incorrect, we propose criteria for a re-evaluation of available data and guidance for further investigations of direct organ effects by SARS-CoV-2.

Proton-Induced X-ray Emission Analysis of Human Autopsy Tissues

1977 ◽  
pp. 403-410 ◽  
Author(s):  
R. D. Lear ◽  
H. A. Van Rinsvelt ◽  
W. R. Adams
Keyword(s):  
Emission Analysis ◽  
X Ray ◽  
Autopsy Tissues ◽  
Human Autopsy

scholarly journals Environmental Cadmium Exposure and Dental Indices in Orthodontic Patients

Healthcare ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 413
Author(s):  
Hui-Ling Chen ◽  
Jason Chen-Chieh Fang ◽  
Chia-Jung Chang ◽  
Ti-Feng Wu ◽  
I-Kuan Wang ◽  
...  
Keyword(s):  
Cadmium Concentration ◽  
Class Ii ◽  
Cadmium Exposure ◽  
Class I ◽  
P Value ◽  
Tooth Decay ◽  
Class Iii ◽  
Orthodontic Patients ◽  
Urine Cadmium ◽  
The Mean

Background. Previous studies have shown that environmental cadmium exposure could disrupt salivary gland function and is associated with dental caries and reduced bone density. Therefore, this cross-sectional study attempted to determine whether tooth decay with tooth loss following cadmium exposure is associated with some dental or skeletal traits such as malocclusions, sagittal skeletal pattern, and tooth decay. Methods. Between August 2019 and June 2020, 60 orthodontic patients with no history of previous orthodontics, functional appliances, or surgical treatment were examined. The patients were stratified into two groups according to their urine cadmium concentrations: high (>1.06 µg/g creatinine, n = 28) or low (<1.06 µg/g creatinine, n = 32). Results. The patients were 25.07 ± 4.33 years old, and most were female (female/male: 51/9 or 85%). The skeletal relationship was mainly Class I (48.3%), followed by Class II (35.0%) and Class III (16.7%). Class I molar relationships were found in 46.7% of these patients, Class II molar relationships were found in 15%, and Class III molar relationships were found in 38.3%. The mean decayed, missing, and filled surface (DMFS) score was 8.05 ± 5.54, including 2.03 ± 3.11 for the decayed index, 0.58 ± 1.17 for the missing index, and 5.52 ± 3.92 for the filled index. The mean index of complexity outcome and need (ICON) score was 53.35 ± 9.01. The facial patterns of these patients were within the average low margin (26.65 ± 5.53 for Frankfort–mandibular plane angle (FMA)). There were no significant differences in the above-mentioned dental indices between patients with high urine cadmium concentrations and those with low urine cadmium concentrations. Patients were further stratified into low (<27, n = 34), average (27–34, n = 23), and high (>34, n = 3) FMA groups. There were no statistically significant differences in the urine cadmium concentration among the three groups. Nevertheless, a marginally significant p-value of 0.05 for urine cadmium concentration was noted between patients with low FMA and patients with high FMA. Conclusion. This analysis found no association between environmental cadmium exposure and dental indices in our orthodontic patients.

scholarly journals Genetic loci regulating cadmium content in rice grains

Euphytica ◽  
2021 ◽  
Vol 217 (3) ◽  
Author(s):  
Gareth J. Norton ◽  
Anthony Travis ◽  
Panthita Ruang-areerate ◽  
Graeme W. Nicol ◽  
Ayotunde A. Adeosun ◽  
...  
Keyword(s):  
Water Treatment ◽  
Water Regime ◽  
Field Experiments ◽  
Cadmium Concentration ◽  
Chromosome 9 ◽  
Chromosome 7 ◽  
Cadmium Content ◽  
Rice Grains ◽  
Water Treatments ◽  
Grain Cadmium

AbstractIt has been estimated that up to 90% of human exposure to cadmium is through food, and that cadmium within rice grains can be a major contributor to that dietary source. In this study genome wide association mapping was conducted on the Bengal and Assam Aus Panel (BAAP) of rice to identify quantitative trait loci and candidate genes for lowering grain cadmium. Field experiments were conducted over two years under two different irrigation systems: continually flooded and alternate wetting and drying (AWD). There was significant effects of water treatment, genotype, and genotype by water treatment interaction. Importantly, AWD increased grain cadmium, on average, by 49.6% and 108.8% in year 1 and 2 respectively. There was between 4.6 and 28 fold variation in cadmium concentration. A total of 58 QTLs were detected but no loci are clearly specific to one water regime despite approximately 20% of variation attributable to genotype by water regime interaction. A number of QTLs were consistent across most water treatments and years. These included QTLs on chromosome 7 (7.23–7.61, 8.93–9.04, and 29.12–29.14 Mbp), chromosome 5 (8.66–8.72 Mbp), and chromosome 9 (11.46–11.64 Mbp). Further analysis of the loci on chromosome 7 (8.93–9.04 Mbp), identified the candidate gene OsNRAMP1, where cultivars with a deletion upstream of the gene had higher concentrations of cadmium compared to the cultivars that did not have the deletion. The distribution of alleles within the BAAP suggest this QTL is easily detected in this population because it is composed of aus cultivars. Local genome cluster analysis suggest high Cd alleles are uncommon, but should be avoided in breeding.

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