Microbiology in minimally invasive autopsy: best techniques to detect infection. ESGFOR (ESCMID study group of forensic and post-mortem microbiology) guidelines

Our study aimed to determine pain levels and the state of welfare connected to laser-based procedures in the treatment of patients diagnosed with uncontrolled glaucoma. The study group included 100 eyes of 100 patients diagnosed with glucoma, 50 of them being treated with micropulse transscleral laser cyclophotocoagulation, and the other 50 eyes being treated with continuous transscleral laser cyclophotocoagulation. We used visual analog scale to gather information from each patient. After analysing the individual information the following results were obtained: the pain level for the micropulse transscleral laser cyclophotocoagulation was 60.23 mm, signifying moderate pain; and the pain score for the continuous transscleral laser cyclophotocoagulation was 76.34 mm, corresponding to moderate-intense pain. Pain level generated by minimally invasive laser procedures is discussed.

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SARS-CoV-2 Pneumonia Phenotyping on Imaging: Correlation with Minimally Invasive Autopsy

SSRN Electronic Journal ◽

10.2139/ssrn.3734288 ◽

2020 ◽

Author(s):

Marcel Koenigkam Santos ◽

Danilo Wada ◽

Maira Benatti ◽

Li Siyuan ◽

Sabrina Batah ◽

...

Keyword(s):

Minimally Invasive ◽

Minimally Invasive Autopsy

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Minimally Invasive Autopsy Practice in COVID-19 Cases: Biosafety and Findings

Pathogens ◽

10.3390/pathogens10040412 ◽

2021 ◽

Vol 10 (4) ◽

pp. 412

Author(s):

Natalia Rakislova ◽

Lorena Marimon ◽

Mamudo R. Ismail ◽

Carla Carrilho ◽

Fabiola Fernandes ◽

...

Keyword(s):

Minimally Invasive ◽

Cause Of Death ◽

Diffuse Alveolar Damage ◽

The Body ◽

Protective Equipment ◽

Safe Alternative ◽

Minimally Invasive Autopsy ◽

Postmortem Studies ◽

Death Investigation ◽

Insight Into

Postmortem studies are crucial for providing insight into emergent diseases. However, a complete autopsy is frequently not feasible in highly transmissible diseases due to biohazard challenges. Minimally invasive autopsy (MIA) is a needle-based approach aimed at collecting samples of key organs without opening the body, which may be a valid alternative in these cases. We aimed to: a) provide biosafety guidelines for conducting MIAs in COVID-19 cases, b) compare the performance of MIA versus complete autopsy, and c) evaluate the safety of the procedure. Between October and December 2020, MIAs were conducted in six deceased patients with PCR-confirmed COVID-19, in a basic autopsy room, with reinforced personal protective equipment. Samples from the lungs and key organs were successfully obtained in all cases. A complete autopsy was performed on the same body immediately after the MIA. The diagnoses of the MIA matched those of the complete autopsy. In four patients, COVID-19 was the main cause of death, being responsible for the different stages of diffuse alveolar damage. No COVID-19 infection was detected in the personnel performing the MIAs or complete autopsies. In conclusion, MIA might be a feasible, adequate and safe alternative for cause of death investigation in COVID-19 cases.

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Assessing viability of a minimally invasive autopsy technique in ascertaining the probable cause of death in patients who were SARS CoV19 positive at the time of their demise

Surgical and Experimental Pathology ◽

10.1186/s42047-021-00094-3 ◽

2021 ◽

Vol 4 (1) ◽

Author(s):

Ajay H. Bhandarwar ◽

Girish D. Bakhshi ◽

Eham Arora ◽

Nikhil Dhimole ◽

Sanjay R. Bijwe ◽

...

Keyword(s):

Minimally Invasive ◽

Cause Of Death ◽

Histopathological Examination ◽

Health Hazard ◽

World Health ◽

Invasive Technique ◽

Tissue Samples ◽

Conventional Autopsy ◽

Minimally Invasive Autopsy ◽

Health Organization

Abstract Background SARS CoV-19 was declared as a pandemic by the World Health Organization (WHO), raising up challenges on various levels ranging from therapeutics to diagnostics. The conventional autopsy technique may pose a health hazard to health care workers. A minimally invasive autopsy technique can diminish this hazard. Materials and methods Between August and November 2020, 51 patients who were suffering from Covid-19 at the time of their demise were included. A novel minimally invasive ultrasound-guided technique for procuring tissue samples of major organs was employed which were thereafter subject to histopathological examination. A detailed review of the course in hospital was noted. An analysis was performed to correlate the cause of death ascertained from our minimally invasive technique with the cause of death ascertained clinically. Results There was adequate tissue sampling in 45 cases, where the minimally invasive autopsy technique confirmed the cause of death in all 45 cases (100%) and made it more specific in 5 cases (11.11%). Conclusion Minimally Invasive Autopsy is an easily reproducible technique which has the potential to strengthen the probable the cause of death with reasonable certainty while ensuring safety and ethics.

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