Study of Intra-Varietal Genetic Variability in Grapevine Cultivars by PCR-Derived Molecular Markers and Correlations with the Geographic Origins

The behavior of six citrus rootstocks, Volkameriana, Citrumelo ‘Swingle’, Citrange ‘Carrizo’, Poncirus trifoliata ‘Serra’, Poncirus trifoliata ‘Rubidoux’ and Poncirus trifoliata ‘Flying Dragon’, in in vitro propagation was studied and compared for shoot proliferation and rooting. In addition, the genetic relationships among the rootstocks studied and other Citrus species, using the Inter-Simple Sequence Repeats (ISSR) molecular markers, were investigated. Nodal explants of three months old shoots were used in Murashige and Skoog medium supplemented with N6-benzyladenine (BA) for shoot proliferation and with naphthaleneacetic acid (NAA) for rooting. The rootstock Volkameriana showed a statistically significant higher number of shoots (1.81), shoot length (15.14 mm) and number of leaves per explant (5.81), while all three Poncirus trifoliata rootstocks showed the lowest numbers. The number of roots and root length per explant were evaluated at the end of the rooting phase. The rootstock ‘Swingle’ showed a higher number of roots per explant (4.2) followed by ‘Flying Dragon’ (3.93) and ‘Carrizo’ (3.23) rootstocks. The rootstocks ‘Swingle’ (140.8 mm), Volkameriana (148 mm) and ‘Flying Dragon’ (131.12 mm) had significantly higher root length per explant compared to ‘Carrizo’ (31 mm) and ‘Rubidoux’ (34.5 mm). The ISSR molecular marker technique used in the present study grouped successfully the different species, varieties and rootstocks studied, revealing their genetic variability. The genetic variability observed among the rootstocks ranged between 0.29 (Poncirus trifoliata ‘Serra’ and Citrumelo ‘Swingle’) and 0.60 (Volkameriana and Citrumelo ‘Swingle’). The response of the rootstocks studied in in vitro propagation however is not related to their genetic affinity.

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Genetic variability in physic nuts cultivated in Northeastern Brazil

Ciência Rural ◽

10.1590/s0103-84782013005000060 ◽

2013 ◽

Vol 43 (6) ◽

pp. 978-984 ◽

Cited By ~ 3

Author(s):

Vanice Dias Oliveira ◽

Allivia Rouse Carregosa Rabbani ◽

Ana Veruska Cruz da Silva ◽

Ana da Silva Lédo

Keyword(s):

Molecular Markers ◽

Genetic Structure ◽

Genetic Variability ◽

Genetic Similarity ◽

Rapd Markers ◽

Dna Amplification ◽

Northeastern Brazil ◽

Jaccard Coefficient ◽

Physic Nut ◽

Genetic Structure And Diversity

This research had as objective to characterize genetically individuals of physic nut cultivated in experimental areas in Sergipe, Brazil by means of RAPD molecular markers. Leaves of 40 individuals were collected and DNA was isolated using CTAB 2% method. Were used 30 primers RAPD for DNA amplification, and this data was used to estimate the genetic similarity among the pairs of individuals, using Jaccard coefficient, and group them out for the UPGMA method. Also, the genetic structure and diversity of the populations were assessed using AMOVA. Of the 100 fragments generated, 29 of were polymorphic. A similarity average of 0.54 among the individuals was found and the amplitude similarities varied from 0.18 to 1.00. One of them (U5) was unit clusters and formed by the most divergent individuals. AMOVA indicated that there is more variation within (63%) the population. In conclusion, it was possible verify genetic variability in physic nut using RAPD markers at these experimental areas.

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Genetic diversity analysis of the Greek lentil (Lens culinaris) landrace ‘Eglouvis’ using morphological and molecular markers

Plant Genetic Resources ◽

10.1017/s1479262118000096 ◽

2018 ◽

Vol 16 (5) ◽

pp. 469-477 ◽

Cited By ~ 5

Author(s):

Georgios F. Tsanakas ◽

Photini V. Mylona ◽

Katerina Koura ◽

Anthoula Gleridou ◽

Alexios N. Polidoros

Keyword(s):

Genetic Diversity ◽

Molecular Markers ◽

Genetic Variability ◽

Morphological Analysis ◽

Phenotypic Variability ◽

Market Price ◽

Genetic Distances ◽

Reaction Products ◽

Principal Coordinates Analysis ◽

Principal Coordinates

AbstractThe Greek lentil landrace ‘Eglouvis’ is cultivated continuously at the Lefkada island for more than 400 years. It has great taste, high nutritional value and high market price. In the present study, we used morphological and molecular markers to estimate genetic diversity within the landrace. Morphological analysis was based on characteristics of the seed. Molecular analysis was performed using simple sequence repeat (SSR) molecular markers in a high-resolution melting (HRM) approach. ‘Samos’ and ‘Demetra’, two of the most widely cultivated commercial lentil varieties in Greece, were used for comparisons. Morphological analysis was performed with 584 seeds randomly selected from a lot. Analysis of seed dimensions and colour distributed the samples in different categories and highlighted the phenotypic variability in ‘Eglouvis’ lentil seeds. Genetic variability was estimated from 91 individual DNA samples with 11 SSR markers using HRM analysis. Genotyping was based upon the shape of the melting curves and the difference plots; all polymerase chain reaction products were also run on agarose gels. Genetic distances of individuals and principal coordinates analysis suggested that ‘Eglouvis’ landrace has a unique genetic background that significantly differs from ‘Samos’ and ‘Demetra’ and no overlapping could be detected. Genetic variability within the ‘Eglouvis’ landrace can be considered in targeted breeding programs as a significant phytogenetic resource of lentils in Greece.

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High Levels of Genetic Variability in an Isolated Colony of Rock-wallabies (Petrogale assimilis): Evidence from Three Classes of Molecular Markers

Australian Journal of Zoology ◽

10.1071/zo96058 ◽

1997 ◽

Vol 45 (2) ◽

pp. 199 ◽

Cited By ~ 12

Author(s):

Peter B. S. Spencer ◽

Mark Adams ◽

Helene Marsh ◽

David J. Miller ◽

Mark D. B. Eldridge

Keyword(s):

Genetic Variation ◽

Molecular Markers ◽

Genetic Variability ◽

Mating Systems ◽

Selective Pressure ◽

Multiple Mating ◽

Dna Fingerprints ◽

Average Heterozygosity ◽

Low Levels ◽

High Level

Estimates of genetic variation for a small (Ne = 39) colony of allied rock-wallabies (Petrogale assimilis) were calculated with three different categories of molecular marker. Average heterozygosity was estimated at 3·8% for allozymes, 47·3% for multilocus ‘DNA fingerprints’ and 85·5% for microsatellite markers. Overall these values indicate that this small isolated colony of rock-wallabies maintains a high level of genetic variation despite its relative isolation and the apparently low levels of migration between colonies. It is likely that mechanisms exist (such as kin avoidance, multiple mating systems, high and variable selective pressure in extreme and fluctuating environmental conditions) that promote the maintenance of high levels of genetic variation in isolated colonies of P. assimilis. These mechanisms are discussed in the context of the results obtained from the molecular markers.

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Population genetic study in Juglans regia L. (Persian walnut) and its taxonomic status within the genus Juglans L.

Phytotaxa ◽

10.11646/phytotaxa.376.4.1 ◽

2018 ◽

Vol 376 (4) ◽

pp. 154 ◽

Cited By ~ 1

Author(s):

MEGHDAD MAGHSOODI ◽

MASOUD SHEIDAI ◽

FAHIMEH KOOHDAR

Keyword(s):

Molecular Markers ◽

Genetic Structure ◽

Genetic Variability ◽

Genetic Study ◽

Population Genetic ◽

Juglans Regia ◽

Its Sequences ◽

Population Genetic Study ◽

Nrdna Its ◽

Persian Walnut

Juglans regia commonly known as Persian walnut of the genus Juglans (Juglandaceae) is cultivated throughout the temperate regions of the world for its high quality wood and edible nuts. Though Persian walnut grows on 70,000 ha in Iran, we have no detailed information on genetic structure of walnut cultivars in the country. A detailed knowledge of genetic diversity and spatial genetic structure is essential for conservation and management of tree species. The species like Persian walnut, which has wide range of geographical distribution, should harbor extensive genetic variability to adapt to environmental fluctuations they face. Therefore, detailed population genetic study of local populations become important for conservation and breeding studies. The main aim of the present study was to investigate the population genetic structure of seven Persian walnut populations including 3 wild and 4 cultivated populations by using ISSR and SRAP molecular markers. We also aimed to compare the genetic variability revealed by ISSR neutral multilocus marker and nrDNA ITS sequences. Finally, we tried to investigate the species relationship within the genus Juglans L. by using molecular phylogeny methods based on nrDNA ITS sequences. The results showed that both multilocus molecular markers and ITS sequences can differentiate Persian walnut populations. The studied populations differed genetically and showed isolation by distance (IBD).

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