Analytical Methods Applied to the Chemical Characterization and Antioxidant Properties of Three Wild Edible Mushroom Species from Northeastern Portugal

Macrolepiota africana is a fungus used as food in Gabon for its culinary properties. However, its nutritional value, mycochemical contents, antioxidant properties and health potential still unknown. The present study investigated the phytochemicals and antioxidant properties of this mushroom species. This chemical screening was followed by a study of the antioxidant activity and a prediction of additional pharmacological activities of M. africana. Using standard methodology, the mycochemical analyses were carried out on aqueous, hydro-ethanolic and ethanolic fungi extracts. The antioxidant activity of the mushroom extracts was determined using DPPH radical scavenging assay. Apart from Digitoxigenine, flavonol and gitoxigenine that were not found in any of the extracts, all other tested mycochemical were found in atleast one of the extracts. Alkaloids, flavonoids, polyphenols, oses and holosides, proanthocyanidins and coumarins were found in all extracts at different intensity. Saponosids, sterols and triterpenes, tannins gallics, reducing sugar, anthracenosides and digitoxine were found in two extracts while tannins catechics, flavone and gitoxine were detected only in one extract. The dosage of phenolic compounds confirmed the richness of this fungus in total polyphenols, its moderate richness in proanthocyanidins, the lack of flavonoids in the aqueous extract and the moderate richness of the hydro-ethanolic and ethanolic extracts in flavonoids. Regarding the antioxidant activities, the results obtained for the DPPH trapping test showed that the different extracts had low to moderate antioxidant activity with antioxidant activity index (IAA) ranged 0.29 to 0.97 respectively in aqueous and ethanolic extracts. The presence of these mycochemical compounds along with the identified antioxidant activities shows that this M. africana have some pharmacological potential.

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Fatty Acid Compositions of Five Wild Edible Mushroom Species Collected from Turkey

International Journal of Pharmacology ◽

10.3923/ijp.2012.463.466 ◽

2012 ◽

Vol 8 (5) ◽

pp. 463-466 ◽

Cited By ~ 4

Author(s):

Pelin Gunc Ergon ◽

Bulent Ergonul ◽

Fatih Kalyoncu ◽

Ilgaz Akata

Keyword(s):

Fatty Acid ◽

Edible Mushroom ◽

Mushroom Species ◽

Wild Edible Mushroom ◽

Fatty Acid Compositions

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Chemical Characterization, Antioxidant, Enzyme Inhibition and Antimutagenic Properties of Eight Mushroom Species: A Comparative Study

Journal of Fungi ◽

10.3390/jof6030166 ◽

2020 ◽

Vol 6 (3) ◽

pp. 166

Author(s):

Sinan Alkan ◽

Ahmet Uysal ◽

Giyasettin Kasik ◽

Sanja Vlaisavljevic ◽

Sanja Berežni ◽

...

Keyword(s):

Chemical Composition ◽

Antioxidant Enzyme ◽

Antioxidant Properties ◽

Reducing Power ◽

Chemical Characterization ◽

Metabolic Activation ◽

Cholinesterase Inhibition ◽

Mushroom Species ◽

Bioactive Agents ◽

Inhibition Ability

This study aimed to determine the chemical composition and biologic activities of eight mushroom species (Amanita crocea, Hemileccinum depilatum, Cyclocybe cylindracea, Lactarius deliciosus, Hygrocybe acutoconica, Neoboletus erythropus, Russula aurea and Russula sanguinea). The antioxidant, enzyme inhibitory and mutagenic/antimutagenic activities were evaluated to provide data on the biologic activities. With respect to the chemical composition, LC–MS/MS technique was used to determine individual phenolic compounds present in the extracts. Antioxidant properties were investigated by different chemical methods including radical quenching (DPPH and ABTS), reducing power (CUPRAC and FRAP), phosphomolybdenum and metal chelating. In the enzyme inhibitory assays, cholinesterases, tyrosinase, amylase and glucosidase were used. Mutagenic and antimutagenic properties were evaluated by the Ames assay. In general, the best antioxidant abilities were observed from H. depilatum and N. erythropus, which also showed highest level of phenolics. The best cholinesterase inhibition ability was found from C. cylindracea (1.02 mg GALAE/g for AChE; 0.99 mg GALAE/g for BChE). Tyrosinase inhibition ability varied from 48.83 to 54.18 mg KAE/g. The extracts exhibited no mutagenic effects and showed significant antimutagenic potential. H. acutoconica, in particular depicted excellent antimutagenicity with a ratio of 97% for TA100 and with a rate of 96% for TA98 strain against mutagens in the presence of metabolic activation system. Results presented in this study tend to show that the mushroom species could be exploited as potential sources of therapeutic bioactive agents, geared towards the management of oxidative stress, global health problems and cancer.

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Antioxidant properties of different edible mushroom species and increased bioconversion efficiency of Pleurotus eryngii using locally available casing materials

Food Chemistry ◽

10.1016/j.foodchem.2012.12.001 ◽

2013 ◽

Vol 138 (2-3) ◽

pp. 1557-1563 ◽

Cited By ~ 46

Author(s):

K.K. Mishra ◽

R.S. Pal ◽

R. ArunKumar ◽

C. Chandrashekara ◽

S.K. Jain ◽

...

Keyword(s):

Antioxidant Properties ◽

Edible Mushroom ◽

Pleurotus Eryngii ◽

Mushroom Species

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Antioxidant Properties of the Wild Edible Mushroom Lactarius salmonicolor

Journal of Medicinal Food ◽

10.1089/jmf.2012.0297 ◽

2013 ◽

Vol 16 (8) ◽

pp. 760-764 ◽

Cited By ~ 13

Author(s):

Georgios Athanasakis ◽

Nektarios Aligiannis ◽

Zacharoula Gonou-Zagou ◽

Alexios-Leandros Skaltsounis ◽

Nikolas Fokialakis

Keyword(s):

Antioxidant Properties ◽

Edible Mushroom ◽

Wild Edible Mushroom

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AMINO ACID COMPOSITION AND ANTIOXIDANT PROPERTIES OF FIVE EDIBLE MUSHROOM SPECIES FROM KASTAMONU, TURKEY

African Journal of Traditional Complementary and Alternative Medicines ◽

10.21010/ajtcamv15i2.10 ◽

2018 ◽

Vol 15 (2) ◽

pp. 80

Author(s):

Temel kan Bakır ◽

Mansor Boufars ◽

Mertcan Karadeniz ◽

Sabri Ünal

Keyword(s):

Antioxidant Activity ◽

Amino Acid ◽

Free Amino ◽

Phenolic Content ◽

Antioxidant Properties ◽

Edible Mushroom ◽

Total Phenolic ◽

Mushroom Species ◽

Boletus Edulis ◽

Ic50 Values

Background: Hydnum repandum, Cantharellus cibarius, Ramaria fennica, Boletus edulis, and Craterellus cornucopioides are all wild edible mushroom species from the Kastamonu province. The aim of this study was to investigate antioxidant properties and amino acid contents of these mushrooms. Materials and Methods: The mushrooms were analyzed for their free amino acid compositions using a high performance Amino Acid analyzer (Biochrom 30). Also, antioxidant activity and total phenolic concentrations of five different mushroom species were studied by using spectrophotometric methods. Results: The mushrooms contained 17 amino acids (g/100 g): Glutamic acid (2.56–1.11), Alanine (1.49–0.54), Arginine (1.62–6.77), Aspartic acid (1.45–0.81), Leucine (1.08–0.64), Methionine (1.05–0.06), Valine (1.05–0.66), Lysine (1.01–0.57), Serine (0.68–0.38), Cysteine (0.88–0.11), Isoleucine (0.61–0.39), Glycine (0.82–0.35), Threonine (0.82–0.44), Phenylalanine (0.66–0.42), Proline (0.60–0.47), Tyrosine (0.58–0.38), and Histidine (0.48–0.22). The total free amino acid (TAA) contents ranged from 169.2 mg/g in Boletus edulis to 89.1 mg/g in Hydnum repandum. These five different mushroom species contain eight essential amino acid species except tryptophan. The antioxidant activity of mushroom extracts was expressed as the percentage of DPPH radical inhibition and IC50 values (mg/mL). The percentage of inhibition ranged from 2.38% to 88.05% and IC50 values ranged from 0.03 to 13.98 mg/mL. The total phenolic content ranged from 0.66 to 7.74 mg/g of dry mushroom extract, expressed as gallic acid equivalents. Conclusion: Methanolic extract of Boletus edulis showed the highest phenolic content and strong antioxidant activity. As a result, the significant linear correlation between the values for the total phenolic content and antioxidant activity of mushroom extracts was corroborated in all of the investigated mushroom species, with the exception of Craterellus cornucopioides.

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