A Novel Staphylococcal Enterotoxin Q Immunosensor Prepared with Self-Assembly Method Based on Horseradish Peroxidase and Double-Layer Gold Nanoparticles

2016 ◽  
Vol 10 (4) ◽  
pp. 892-899 ◽  
Author(s):  
Lihui Wei ◽  
Xingya Wang ◽  
Dingqiang Lu ◽  
Yang Li ◽  
Guangchang Pang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Horseradish Peroxidase ◽  
Double Layer ◽  
Self Assembly ◽  
Staphylococcal Enterotoxin ◽  
Assembly Method

scholarly journals Selective Detection of Human Lung Adenocarcinoma Cells Based on the Aptamer-Conjugated Self-Assembled Monolayer of Gold Nanoparticles

Micromachines ◽  
2019 ◽  
Vol 10 (3) ◽  
pp. 195 ◽  
Author(s):  
Ngoc-Viet Nguyen ◽  
Chun-Ping Jen
Keyword(s):  
Gold Nanoparticles ◽  
Self Assembly ◽  
Human Lung ◽  
Specific Binding ◽  
Physical And Mechanical Properties ◽  
Target Cells ◽  
Self Assembled Monolayer ◽  
Assembly Method ◽  
Human Lung Adenocarcinoma Cells ◽  
Self Assembled

This study established a microfluidic chip for the capture of A549 human lung circulating tumor cells via the aptamer-conjugated self-assembled monolayer (SAM) of gold nanoparticles (AuNPs) in the channel. AuNPs are among the most attractive nanomaterials for the signal enhancement of biosensors owing to their unique chemical, physical, and mechanical properties. The microchip was fabricated using soft photolithography and casting and molding techniques. A self-assembly method was designed to attach AuNPs, cell-specific aptamers, and target cells onto the desired area (i.e., SAM area). In this study, the gold microelectrode configuration was characterized by fluorescence microscopy and impedance measurements to confirm the important modification steps. Subsequently, several investigations with the proposed assay were conducted with different cell samples to determine the specific binding ability of the device for A549 adenocarcinoma cancer cells. This work has ensured a simple, convenient, selective, and sensitive approach for the development of biosensors for lung cancer detection during the early stages.

Large mesoporous carbons decorated with silver and gold nanoparticles by a self-assembly method: enhanced electrocatalytic activity for H2O2 electroreduction and sodium nitrite electrooxidation

RSC Advances ◽  
2015 ◽  
Vol 5 (4) ◽  
pp. 2956-2962 ◽  
Author(s):  
X. J. Yang ◽  
Y. H. Wang ◽  
J. Bai ◽  
X. Y. He ◽  
X. E. Jiang
Keyword(s):  
Gold Nanoparticles ◽  
Sodium Nitrite ◽  
Self Assembly ◽  
Electrocatalytic Activity ◽  
Mesoporous Carbon ◽  
Mesoporous Carbons ◽  
Silver And Gold Nanoparticles ◽  
Assembly Method

The silver, gold nanoparticles were grown onto poly (diallyldimethyl ammoniumchloride, PDDA)-functionalized large mesoporous carbon (LMC) by simple self-assembly method. AuNPs or AgNPs/PDDA–LMC show superior electrocatalytic activity.

scholarly journals Layer by Layer Assembled Chitosan-Coated Gold Nanoparticles for Enhanced siRNA Delivery and Silencing

2021 ◽  
Vol 22 (2) ◽  
pp. 831
Author(s):  
Elnaz Shaabani ◽  
Maryam Sharifiaghdam ◽  
Herlinde De Keersmaecker ◽  
Riet De Rycke ◽  
Stefaan De Smedt ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Self Assembly ◽  
Fluorescent Protein ◽  
Transfection Efficiency ◽  
Direct Reduction ◽  
Sirna Delivery ◽  
Lung Epithelial Cells ◽  
Endosomal Escape ◽  
Layer By Layer ◽  
Assembly Method

Delivery of small interfering RNA (siRNA) provides one of the most powerful strategies for downregulation of therapeutic targets. Despite the widely explored capabilities of this strategy, intracellular delivery is hindered by a lack of carriers that have high stability, low toxicity and high transfection efficiency. Here we propose a layer by layer (LBL) self-assembly method to fabricate chitosan-coated gold nanoparticles (CS-AuNPs) as a more stable and efficient siRNA delivery system. Direct reduction of HAuCl4 in the presence of chitosan led to the formation of positively charged CS-AuNPs, which were subsequently modified with a layer of siRNA cargo molecules and a final chitosan layer to protect the siRNA and to have a net positive charge for good interaction with cells. Cytotoxicity, uptake, and downregulation of enhanced Green Fluorescent Protein (eGFP) in H1299-eGFP lung epithelial cells indicated that LBL-CS-AuNPs provided excellent protection of siRNA against enzymatic degradation, ensured good uptake in cells by endocytosis, facilitated endosomal escape of siRNA, and improved the overall silencing effect in comparison with commercial transfection reagents Lipofectamine and jetPEI®. Therefore, this work shows that LBL assembled CS-AuNPs are promising nanocarriers for enhanced intracellular siRNA delivery and silencing.

Preparation of Gold Nanoparticles Deposited Silicon Thin Film Electrode by Self-Assembly Method for the Employment of an Anode Material for Lithium Secondary Batteries

2015 ◽  
Vol 15 (10) ◽  
pp. 8222-8227 ◽  
Author(s):  
Martin Halim ◽  
Jung Sub Kim ◽  
Si Hieu Nguyen ◽  
Bup Ju Jeon ◽  
Joong Kee Lee
Keyword(s):  
Thin Films ◽  
Gold Nanoparticles ◽  
Anode Material ◽  
Self Assembly ◽  
Silicon Surface ◽  
Sodium Citrate ◽  
Reversible Capacity ◽  
Lithium Secondary Batteries ◽  
Silicon Thin Films ◽  
Assembly Method

This work describes a self-assembly method of gold nanoparticles coating on the surface of silicon thin films for the anode material of lithium secondary batteries. The preparation of the silicon thin films was carried out by electron cyclotron resonance metal organic chemical vapor deposition (ECR-MOCVD) process. The obtained films were further coated with (3-aminopropyl)-trimethoxysilane (APTMS) which has a role to bind the oxygen functional groups on Si surface and the gold nanoparticles. The dispersed gold nanoparticles on the surface of silicon thin films could be prepared due to self-assembly phenomena which interact between attraction and repulsion in gold nanoparticles colloidal solution (GNCS). The use of reducing agent of sodium citrate and tannic acid in GNCS significantly affected the size of gold nanoparticle in our experimental range. Based on our experimental results, the higher reversible capacity was exhibited for the silicon that was immersed in the GNCS consisted of only sodium citrate. The GNCS consisted of both sodium citrate and tannic acid produced severe coagulated nanoparticles when deposited on the silicon surface and thus inhibited the lithium movement from electrolyte to silicon surface. Consequently, the reversible capacity of silicon anode material with coagulated gold nanoparticles coating showed the reduced performance.

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