scholarly journals Layer by Layer Assembled Chitosan-Coated Gold Nanoparticles for Enhanced siRNA Delivery and Silencing

2021 ◽  
Vol 22 (2) ◽  
pp. 831
Author(s):  
Elnaz Shaabani ◽  
Maryam Sharifiaghdam ◽  
Herlinde De Keersmaecker ◽  
Riet De Rycke ◽  
Stefaan De Smedt ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Self Assembly ◽  
Fluorescent Protein ◽  
Transfection Efficiency ◽  
Direct Reduction ◽  
Sirna Delivery ◽  
Lung Epithelial Cells ◽  
Endosomal Escape ◽  
Layer By Layer ◽  
Assembly Method

Delivery of small interfering RNA (siRNA) provides one of the most powerful strategies for downregulation of therapeutic targets. Despite the widely explored capabilities of this strategy, intracellular delivery is hindered by a lack of carriers that have high stability, low toxicity and high transfection efficiency. Here we propose a layer by layer (LBL) self-assembly method to fabricate chitosan-coated gold nanoparticles (CS-AuNPs) as a more stable and efficient siRNA delivery system. Direct reduction of HAuCl4 in the presence of chitosan led to the formation of positively charged CS-AuNPs, which were subsequently modified with a layer of siRNA cargo molecules and a final chitosan layer to protect the siRNA and to have a net positive charge for good interaction with cells. Cytotoxicity, uptake, and downregulation of enhanced Green Fluorescent Protein (eGFP) in H1299-eGFP lung epithelial cells indicated that LBL-CS-AuNPs provided excellent protection of siRNA against enzymatic degradation, ensured good uptake in cells by endocytosis, facilitated endosomal escape of siRNA, and improved the overall silencing effect in comparison with commercial transfection reagents Lipofectamine and jetPEI®. Therefore, this work shows that LBL assembled CS-AuNPs are promising nanocarriers for enhanced intracellular siRNA delivery and silencing.

scholarly journals Surface-Initiated Atom Transfer Polymerized Anionic Corona on Gold Nanoparticles for Anti-Cancer Therapy

Pharmaceutics ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 261
Author(s):  
Wei Mao ◽  
Sol Lee ◽  
Ji Un Shin ◽  
Hyuk Sang Yoo
Keyword(s):  
Gold Nanoparticles ◽  
Transfection Efficiency ◽  
Cancer Therapeutics ◽  
Atom Transfer ◽  
Layer By Layer ◽  
Dependent Manner ◽  
Metallic Particles ◽  
Anti Cancer

Surface initiated atom transfer radical polymerization (SI-ATRP) documented a simple but efficient technique to grow a dense polymer layer on any surface. Gold nanoparticles (AuNPs) give a broad surface to immobilize sulfhyryl group-containing initiators for SI-ATRP; in addition, AuNPs are the major nanoparticulate carriers for delivery of anti-cancer therapeutics, since they are biocompatible and bioinert. In this work, AuNPs with a disulfide initiator were polymerized with sulfoethyl methacrylate by SI-ATRP to decorate the particles with anionic corona, and branched polyethyeleneimine (PEI) and siRNA were sequentially layered onto the anionic corona of AuNP by electrostatic interaction. The in vitro anti-cancer effect confirmed that AuNP with anionic corona showed higher degrees of apoptosis as well as suppression of the oncogene expression in a siRNA dose-dependent manner. The in vivo study of tumor-bearing nude mice revealed that mice treated with c-Myc siRNA-incorporated AuNPs showed dramatically decreased tumor size in comparison to those with free siRNA for 4 weeks. Furthermore, histological examination and gene expression study revealed that the decorated AuNP significantly suppressed c-Myc expression. Thus, we envision that the layer-by-layer assembly on the anionic brushes can be potentially used to incorporate nucleic acids onto metallic particles with high transfection efficiency.

Fabrication of microelectrodes using flow layer-by-layer self assembly of gold nanoparticles

2012 ◽  
Vol 52 (5) ◽  
pp. 1043-1051 ◽  
Author(s):  
Panittamat Kumlangdudsana ◽  
Adisorn Tuantranont ◽  
Stephan Thierry Dubas ◽  
Luxsana Dubas
Keyword(s):  
Gold Nanoparticles ◽  
Self Assembly ◽  
Layer By Layer

Hydrogen-Bonding Layer Protecting Polyelectrolyte Capsules and Its Mechanical Property Study with Atomic Force Microscope

2008 ◽  
Vol 8 (6) ◽  
pp. 2996-3002 ◽  
Author(s):  
Liqin Ge ◽  
Xing Wang ◽  
Long Ba ◽  
Zhongze Gu
Keyword(s):  
Hydrogen Bonding ◽  
Atomic Force Microscope ◽  
Elastic Deformation ◽  
Self Assembly ◽  
Laser Scanning ◽  
Confocal Laser ◽  
Bonding Layer ◽  
Layer By Layer ◽  
Assembly Method ◽  
Atomic Force

The hydrogen-bonding multilayered polyelectrolyte capsules with sizes around 6 μm were fabricated by layer-by-layer self-assembly method. The morphology of the obtained capsules was observed with Scanning Electron Microscope (SEM), Confocal Laser Scanning Microscope (CLSM) and Atomic Force Microscope (AFM), respectively. The elastic properties of the capsules were studied with AFM. The capsule was pressed by cantilever with different lengths, a glass bead glued at the end of the cantilever. The force curves were measured on the capsule in air. The Young's modulus of the capsule was obtained (E = 170 MPa for the loading). Results show that this model can predict the elastic deformation of the microcapsule. The accuracy of the elastic deformation of polymer capsule can be ensured using a cantilever of mediate stiffness. Our results show that the existence of the hydrogen-bonding layer makes the multilayered polyelectrolyte harder in comparison with the pure multilayered polyelectrolyte capsules.

Layer-by-layer self-assembly of 2D graphene nanosheets, 3D copper oxide nanoflowers and 0D gold nanoparticles for ultrasensitive electrochemical detection of alpha fetoprotein

RSC Advances ◽  
2015 ◽  
Vol 5 (70) ◽  
pp. 56583-56589 ◽  
Author(s):  
Yulan Wang ◽  
Dan Wu ◽  
Yong Zhang ◽  
Xiang Ren ◽  
Yaoguang Wang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Copper Oxide ◽  
Electrochemical Detection ◽  
Self Assembly ◽  
Graphene Nanosheets ◽  
Electrochemical Immunosensor ◽  
Alpha Fetoprotein ◽  
Quantitative Detection ◽  
Layer By Layer ◽  
Label Free

In this work, a novel and ultrasensitive label-free electrochemical immunosensor was developed for the quantitative detection of alpha fetoprotein (AFP).

Fabrication of montmorillonite and titanate nanotube based coatings via layer-by-layer self-assembly method to enhance the thermal stability, flame retardancy and ultraviolet protection of polyethylene terephthalate (PET) fabric

RSC Advances ◽  
2016 ◽  
Vol 6 (59) ◽  
pp. 53625-53634 ◽  
Author(s):  
Ying Pan ◽  
Wei Wang ◽  
Haifeng Pan ◽  
Jing Zhan ◽  
Yuan Hu
Keyword(s):  
Thermal Stability ◽  
Polyethylene Terephthalate ◽  
Flame Retardancy ◽  
Self Assembly ◽  
Layer By Layer ◽  
Pet Fabric ◽  
Thermal Oxidative Stability ◽  
Titanate Nanotube ◽  
Assembly Method ◽  
Ultraviolet Protection

Montmorillonite and titanate nanotube based coatings have been prepared through LbL self-assembly method, in order to enhance the thermal and thermal-oxidative stability, flame retardancy and UV protection of polyethylene terephthalate fabric.

Spray-assisted layer-by-layer self-assembly of tertiary-amine-stabilized gold nanoparticles and graphene oxide for efficient CO2 capture

2020 ◽  
Vol 601 ◽  
pp. 117905 ◽  
Author(s):  
Jiwoong Heo ◽  
Moonhyun Choi ◽  
Daheui Choi ◽  
Hyejoong Jeong ◽  
Hyun Young Kim ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Graphene Oxide ◽  
Co2 Capture ◽  
Tertiary Amine ◽  
Self Assembly ◽  
Layer By Layer

A wearable and highly sensitive strain sensor based on a polyethylenimine–rGO layered nanocomposite thin film

2017 ◽  
Vol 5 (31) ◽  
pp. 7746-7752 ◽  
Author(s):  
Xueliang Ye ◽  
Zhen Yuan ◽  
Huiling Tai ◽  
Weizhi Li ◽  
Xiaosong Du ◽  
...  
Keyword(s):  
Thin Film ◽  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Self Assembly ◽  
Strain Sensor ◽  
Layer By Layer ◽  
Nanocomposite Thin Film ◽  
Assembly Method ◽  
Reduced Graphene ◽  
Highly Sensitive

A novel strain sensor based on reduced graphene oxide with ultra-sensitive and ultra-durable performance was fabricated by the chemical layer-by-layer self-assembly method.

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