An Ultrasensitive Fluorescence Immunoassay Based on Magnetic Separation and Upconversion Nanoparticles as Labels for the Detection of Chloramphenicol in Animal-Derived Foods

2020 ◽  
Vol 13 (11) ◽  
pp. 2039-2049
Author(s):  
Wei Sheng ◽  
Na Huang ◽  
Yue Liu ◽  
Biao Zhang ◽  
Wanli Zhang ◽  
...  
2018 ◽  
Vol 10 (44) ◽  
pp. 5313-5320 ◽  
Author(s):  
Wei Sheng ◽  
Wenxia Duan ◽  
Yingjie Shi ◽  
Qing Chang ◽  
Yan Zhang ◽  
...  

NaYF4:Yb/Tm upconversion nanoparticles-based fluorescence immunoassay in combination with magnetic separation for detecting bisphenol A in drinking and river water samples.


RSC Advances ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 517-524
Author(s):  
Chuanyong Li ◽  
Wanlin Sun ◽  
Lianrun Huang ◽  
Nana Sun ◽  
Xiude Hua ◽  
...  

The anti-thiamethoxam and anti-dextran monoclonal antibodies were prepared to develop a multicolor upconversion fluorescence immunoassay for the simultaneous determination of thiamethoxam (544 nm) and dextran (477 nm).


2015 ◽  
Vol 15 (10) ◽  
pp. 7950-7954 ◽  
Author(s):  
Phuong-Diem Nguyen ◽  
Vu Thanh Cong ◽  
Sang Jun Son ◽  
Junhong Min

This study introduces the facile fabrication of a bimodal nanohybrid for the luminescent ON/OFF detection of glutathione. The proposed nanosensor consists of magnetic (Fe3O4) and upconversion nanoparticles (UCP) co-encapsulated in a silica matrix, and decorated with gold nanoparticle (AuNP) as a luminescent quencher. The detection mechanism is based on the Luminescent Resonance Energy Transfer (LRET) between the donor (UCP) and the acceptor (AuNP) with the help of a disulfide bond as a bridging element. In the presence of glutathione, the disulfide bridges between AuNPs and Fe3O4/UCP@SiO2 was cleaved and the amount of glutathione could be traced by the restored luminescence (ON state) of the nanohybrids after magnetic separation.


Sensors ◽  
2021 ◽  
Vol 22 (1) ◽  
pp. 8
Author(s):  
Xufang Huang ◽  
Wei Sheng ◽  
Haonan Chen ◽  
Biao Zhang ◽  
Na Huang ◽  
...  

A competitive fluorescence immunoassay for the quantitative detection of 2-amino-3-methylimidazo [4, 5-f] quinoline (IQ) in pan-fried meat patties was developed, using magnetic nanoparticles coupled with coating antigen as the capture probe and anti-IQ antibody coupled with NaYF4: Yb, Er upconversion nanoparticles as the signal probe. Under optimal conditionals, the wide detection range for IQ in phosphate buffer saline is from 0.01 to 100 μg·L−1 (R2 = 0.991) with a detection limit of 0.007 μg·L−1. This proposed method has been applied to detect IQ in two different types of pan-fried meat patties at varying frying times, and the IQ content in chicken patties and fish patties are 2.11–3.47 μg·kg−1 and 1.35–2.85 μg·kg−1, respectively. These results are consistent with that of the ultraperformance liquid chromatography-tandem mass spectrometry. In summary, this method can serve as a sensitive and specific test tool for the determination of IQ in processed meat.


Nanoscale ◽  
2020 ◽  
Vol 12 (14) ◽  
pp. 7875-7887 ◽  
Author(s):  
Ying Lan ◽  
Xiaohui Zhu ◽  
Ming Tang ◽  
Yihan Wu ◽  
Jing Zhang ◽  
...  

A near-infrared (NIR) activated theranostic nanoplatform based on upconversion nanoparticles (UCNPs) is developed in order to overcome the hypoxia-associated resistance in photodynamic therapy by photo-release of NO upon NIR illumination.


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