Recovery of Biomolecules from Agroindustry by Solid-Liquid Enzyme-Assisted Extraction: a Review

2021 ◽  
Author(s):  
Elisa Hernández Becerra ◽  
Eduardo De Jesús Pérez López ◽  
Jhon Wilder Zartha Sossa
Keyword(s):  
Assisted Extraction ◽  
Solid Liquid ◽  
Enzyme Assisted Extraction

scholarly journals Valorization of Bilberry (Vaccinium myrtillus L.) Pomace by Enzyme-Assisted Extraction: Process Optimization and Comparison with Conventional Solid-Liquid Extraction

Antioxidants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 773
Author(s):  
Michail Syrpas ◽  
Egle Valanciene ◽  
Ernesta Augustiniene ◽  
Naglis Malys
Keyword(s):  
Antioxidant Capacity ◽  
Liquid Extraction ◽  
Vaccinium Myrtillus ◽  
Water Soluble ◽  
Total Phenolic ◽  
Assisted Extraction ◽  
Solid Liquid Extraction ◽  
Solid Liquid ◽  
Vaccinium Myrtillus L ◽  
Enzyme Assisted Extraction

Bilberry (Vaccinium myrtillus L.) pomace contains a significant amount of polyphenols and can serve as a basis for food additives, nutraceuticals, and functional foods. Although various techniques can be employed to recover bioactive fractions from berry pomaces, data on enzyme-assisted extraction (EAE) of bilberry pomace are rather scarce. This study aimed to optimize critical EAE parameters using Viscozyme L to obtain a high-yield extract with enhanced antioxidant capacity. Central composite design and response surface methodology evaluating the effect of four independent variables, namely, pH, temperature, extraction time, and enzyme concentration on three responses, were employed to define optimal EAE conditions. Under the optimal conditions (pH: 4.5, temperature 46 °C, 1 h of extraction, and 2 active units (AU) of Viscozyme L/g of pomace), EAE yielded 56.15 g/ 100 g DW of the water-soluble fraction. Comparison with conventional maceration indicated that EAE, besides the yield, significantly increased the in vitro antioxidant capacity measured by the total phenolic content, ABTS, ORAC, and CUPRAC assays. Moreover, an increase was observed for the measured mono- and disaccharide as well as anthocyanin content. Overall, this study demonstrates the improved efficiency of EAE over conventional solid–liquid extraction to recover fractions with a higher yield and enhanced functional properties in a fast and sustainable manner.

Enzyme-Assisted Extraction of Moniliformin from Extruded Corn Grits

2005 ◽  
Vol 53 (13) ◽  
pp. 5074-5078 ◽  
Author(s):  
Soo-Hyun Chung ◽  
Dojin Ryu ◽  
Eun-Kyung Kim ◽  
Lloyd B. Bullerman
Keyword(s):  
Assisted Extraction ◽  
Enzyme Assisted Extraction

Detection of aflatoxin M1in milk, cheese and sour cream samples from Costa Rica using enzyme-assisted extraction and HPLC

2015 ◽  
Vol 8 (2) ◽  
pp. 128-135 ◽  
Author(s):  
Guadalupe Chavarría ◽  
Fabio Granados-Chinchilla ◽  
Margarita Alfaro-Cascante ◽  
Andrea Molina
Keyword(s):  
Costa Rica ◽  
Assisted Extraction ◽  
Sour Cream ◽  
Enzyme Assisted Extraction

scholarly journals Equilibrium Modeling of Astaxanthin Extraction from Haematococcus pluvialis

2021 ◽  
Vol 21 (3) ◽  
pp. 554
Author(s):  
Putri Restu Dewati ◽  
Rochmadi Rochmadi ◽  
Abdul Rohman ◽  
Avido Yuliestyan ◽  
Arief Budiman
Keyword(s):  
Experimental Data ◽  
High Performance ◽  
Haematococcus Pluvialis ◽  
Equilibrium Constants ◽  
Extraction Methods ◽  
Single Step ◽  
Equilibrium Models ◽  
Extraction Equilibrium ◽  
Assisted Extraction ◽  
Solid Liquid

Astaxanthin is a natural antioxidant, and the highest content of this compound is found in Haematococcus pluvialis microalgae. Microwave-assisted extraction (MAE) is one of the environmentally friendly extraction methods and has many advantages. This study aims to investigate the extraction of astaxanthin through the MAE method using various solvents. Several equilibrium models were proposed to describe this solid-liquid equilibrium. The solid-liquid extraction equilibrium parameters were determined by minimizing the sum of squares of errors (SSE), in which equilibrium constants were needed for scaling up purposes. Previously, the microalgae were pretreated with HCl to soften their cell walls in order to improve the extraction recovery. In this study, dichloromethane, acetone, methanol, and ethanol were used as the solvents for extraction. The astaxanthin concentration was determined by high-performance liquid chromatography (HPLC) and spectrophotometry. Astaxanthin was found to attain equilibrium at 57.42% recovery in a single-step extraction. Thus, several steps were required in sequence to obtain an optimum recovery. The experimental data were fitted to three equilibrium models, namely, Henry, Freundlich, and Langmuir models. The experimental data were well fitted to all the models for the extraction in dichloromethane, methanol, ethanol and acetone, as evident from the almost same SSE value for each model.

scholarly journals RSM based optimized enzyme-assisted extraction of antioxidant phenolics from underutilized watermelon (Citrullus lanatus Thunb.) rind

2014 ◽  
Vol 52 (8) ◽  
pp. 5048-5056 ◽  
Author(s):  
Muhammad Mushtaq ◽  
Bushra Sultana ◽  
Haq Nawaz Bhatti ◽  
Muhammad Asghar
Keyword(s):  
Citrullus Lanatus ◽  
Assisted Extraction ◽  
Enzyme Assisted Extraction
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