Selective degradation of biphenyl and methylbiphenyls in crude oil by two strains of marine bacteria

1983 ◽  
Vol 29 (5) ◽  
pp. 497-503 ◽  
Author(s):  
P. M. Fedorak ◽  
D. W. S. Westlake
Keyword(s):  
Carbon Source ◽  
Benzoic Acid ◽  
Crude Oil ◽  
Marine Sediment ◽  
Marine Bacteria ◽  
Sole Carbon Source ◽  
Bacterial Isolates ◽  
Aromatic Fraction ◽  
Selective Degradation ◽  
Prudhoe Bay

Bacterial isolates were obtained from marine sediment and water enrichment cultures which had been maintained for 3 years by monthly transfers on artificial seawater with Prudhoe Bay crude oil as the sole carbon source. Capillary gas chromatographic analyses showed that two isolates selectively degraded only biphenyl, 3-methylbiphenyl, 4-methylbiphenyl, and three other minor, unidentified compounds in the aromatic fraction. No degradation was detected in the saturate fraction, nor in the sulfur heterocyclic component of the oil. When grown on any of the pure biphenyls, these isolates produced a transient, yellow intermediate which had the spectrophotometric characteristics of α-hydroxymuconic semialdehydes. Growth on either methylbiphenyl compound produced a methylbenzoic acid, indicating that the nonmethylated ring was the first to be cleaved. The isolates, identified as Alcaligenes sp. and Acinetobacter sp., were also able to grow on benzoic acid, 3-, and 4-methylbenzoic acids, indicating that they could further metabolize the aromatic acid intermediates.

scholarly journals Study of a plugging microbial consortium using crude oil as sole carbon source

2008 ◽  
Vol 5 (4) ◽  
pp. 367-374 ◽  
Author(s):  
Jing Wang ◽  
Guiwen Yan ◽  
Mingquan An ◽  
Jieli Liu ◽  
Houming Zhang ◽  
...  
Keyword(s):  
Carbon Source ◽  
Crude Oil ◽  
Sole Carbon Source ◽  
Microbial Consortium

scholarly journals Effect of Bacterial Isolates From Soil Samples on Bisphenol A

2020 ◽  
Vol 31 (1) ◽  
pp. 15
Author(s):  
Dr.Neihaya Heikmat Zaki
Keyword(s):  
Carbon Source ◽  
Bisphenol A ◽  
Sole Carbon Source ◽  
Soil Samples ◽  
Triazine Ring ◽  
Bacterial Isolates ◽  
Optimal Temperature ◽  
Tigris River ◽  
End Products ◽  
Proteus Penneri

Twenty five samples were collected from the soil around the Tigris River from different locations in Iraqi cities, and 45 bacterial isolates were obtained. Three of these isolates were further tested for their degrading capacity of Bisphenol A (BPA) in Basal Mineral Medium, included: Pseudomonas orizohibtanis, Escherishia coli and Proteus penneri. The optimal temperature for the removal of BPA was determined at 20˚C, 37˚ and 45˚C for 1, 5, and 15 days, and the degradation increased up to a temperature of 37°C. Growth test was performed on isolated bacteria with BisPhenol A as the sole carbon source, and with increasing incubation time, the culture grew almost linearly to 24 hours. BPA decreased after 1days after incubating with tested bacterial isolates, and almost broken after 5 days, while it disappeared after 15 days at 37C, and Pseudomonas orizohibtanis exhibited the best degradation of BPA. The absorbance peaks in the UV region appeared at 222 and 276 nm and attributed to the benzene ring and triazine ring respectively. The end products of BPA degradation were analyzed by GCMS after 15 days of incubation. The chromatogram for Pseudomanas orizohibtanis showed three peaks at retention times of 70, 210 and 280 min, and referred to hexasiloxane, heptasiloxane, and Octasiloxane respectively. The present study was aimed to isolate bacteria from the soil of the Tigris River, and determined the ability to degrade Bisphenol-A, and characterized the environmental conditions of bacterial growth, and then analysis the products of the degradation by GC-MS.

UTILIZATION OF AROMATIC HYDROCARBONS BY ARTHROBACTER SPP.

1967 ◽  
Vol 13 (2) ◽  
pp. 205-211 ◽  
Author(s):  
I. L. Stevenson
Keyword(s):  
Carbon Source ◽  
Aromatic Hydrocarbons ◽  
Sole Carbon Source ◽  
Total Population ◽  
Residual Soil ◽  
Bacterial Isolates ◽  
Aromatic Substrates ◽  
Wide Range ◽  
Nutritional Studies ◽  
Different Soils

Bacterial isolates from a number of different soils were screened by growth observation and microscopic examination for Arthrobacter spp. Incidence of arthrobacter in the total population varied, but averaged around 15% in the soils investigated. One hundred and thirty arthrobacter isolates were tested for their ability to utilize aromatic hydrocarbons as their sole carbon source. Seventy-seven percent of these organisms were able to grow on at least two aromatic substrates and many were capable of growth on a wide range of these compounds. Nutritional studies indicated that arthrobacter with simple requirements were able to utilize the greatest number of aromatic hydrocarbons as their sole carbon source. The ability of the arthrobacter to metabolize aromatic compounds is discussed in terms of their possible role in the formation and turnover of residual soil organic matter.

scholarly journals Extra-Heavy Crude Oil Degradation by Alternaria sp. Isolated from Deep-Sea Sediments of the Gulf of Mexico

2021 ◽  
Vol 11 (13) ◽  
pp. 6090
Author(s):  
Lucia Romero-Hernández ◽  
Patricia Velez ◽  
Itandehui Betanzo-Gutiérrez ◽  
María Dolores Camacho-López ◽  
Rafael Vázquez-Duhalt ◽  
...  
Keyword(s):  
Carbon Source ◽  
Gulf Of Mexico ◽  
Crude Oil ◽  
Deep Sea ◽  
Sole Carbon Source ◽  
The United States ◽  
Heavy Crude Oil ◽  
Deep Sea Sediments ◽  
Alternaria Sp ◽  
Heavy Crude

The Gulf of Mexico (GoM) is an important source of oil for the United States and Mexico. There has been growing interest, particularly after the Deepwater Horizon oil spill, in characterizing the fungal diversity of the GoM and identifying isolates for use in the bioremediation of petroleum in the event of another spill. Most studies have focused on light crude oil bioremediation processes, while heavy crude oil (HCO) and extra-heavy crude oil (EHCO) have been largely ignored. In this work, we evaluated the ability of fungal isolates obtained from deep-sea sediments of the Mexican economic exclusive zone (EEZ) of the GoM to degrade HCO (16–20° API) and EHCO (7–10° API). Alternaria sp., Penicillium spp., and Stemphylium sp. grew with HCO as the sole carbon source. Remarkably, Alternaria sp. was the only isolate able to grow with EHCO as the sole carbon source, degrading up to 25.6% of the total EHCO and 91.3% of the aromatic fraction, as demonstrated by gas chromatography analysis of the saturate, aromatic, and polar fractions. These findings proved to be significant, identifying Alternaria sp. as one of the few fungi reported so far capable of degrading untreated EHCO and as a suitable candidate for bioremediation of EHCO in future studies.

scholarly journals Isolation and Potential Testing of North Sumatera Berastagi Agricultural Soil In Degrading Marshal Insecticide With Carbosulfan Active Material

2020 ◽  
Vol 2 (02) ◽  
pp. 147-156
Author(s):  
Nunuk Priyani
Keyword(s):  
Carbon Source ◽  
Agricultural Soil ◽  
Sole Carbon Source ◽  
Active Material ◽  
Bacterial Isolates ◽  
Selective Media

The isolation of bacteria from Berastagi agricultural soil North Sumatera has been done. The aim is to evaluate their ability in degrading carbosulfan. Sixteen bacterial isolates were obtained using selective media Bushnel Hass Agar (BHA) containing 12 ppm of carbosulfan. The parameters observed were the growth of isolates, biosurfactant activity, biosurfactant concentration, and the residue of carbosulfan after 21 days of incubation. The result showed that all isolates were able to degrade carbosulfan as the sole carbon source. Two isolates namely JBM 3 (isolate from citrus agricultural soil Berastagi) and KBM 1 (isolate from cabbage agricultural soil Berastagi) were selected for further test to determine their ability to degrade carbosulfan. The results showed that both of the isolates were able to degrade carbosulfan. Compare to control, isolate JBM 3 was able to decrease the concentration of carbosulfan by 33.33%, while isolate KBM 1 was able to reduce carbosulfan concentration up to 40.47%.

scholarly journals Glyphosate Utilization as the Source of Carbon: Isolation and Identification of new Bacteria

2011 ◽  
Vol 8 (4) ◽  
pp. 1582-1587 ◽  
Author(s):  
M. Mohsen Nourouzi ◽  
T. G. Chuah ◽  
Thomas S. Y. Choong ◽  
C. J. Lim
Keyword(s):  
Carbon Source ◽  
Stenotrophomonas Maltophilia ◽  
Sole Carbon Source ◽  
Enrichment Culture ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Providencia Alcalifaciens ◽  
Mixed Bacteria ◽  
Aminomethyl Phosphonic Acid

Mixed bacteria from oil palm plantation soil (OPS) were isolated to investigate their ability to utilize glyphosate as carbon source. Results showed that approximately all of the glyphosate was converted to aminomethyl-phosphonic acid (AMPA) (99.5%). It is worthy to note that mixed bacteria were able to degrade only 2% of AMPA to further metabolites. Two bacterial strainsi.e. Stenotrophomonas maltophiliaandProvidencia alcalifacienswere obtained from enrichment culture. Bacterial isolates were cultured individually on glyphosate as a sole carbon source. It was observed that both isolates were able to convert glyphosate to AMPA.

Effect of the dispersant Corexit 9527 on the microbial degradation of Prudhoe Bay oil

1982 ◽  
Vol 28 (1) ◽  
pp. 117-122 ◽  
Author(s):  
J. M. Foght ◽  
D. W. S. Westlake
Keyword(s):  
Crude Oil ◽  
Microbial Degradation ◽  
Incubation Time ◽  
Phosphate Solution ◽  
Alkane Degradation ◽  
Marine Oil ◽  
Aromatic Fraction ◽  
Substrate Degradation ◽  
Selective Sensitivity ◽  
Prudhoe Bay

A marine oil-degrading population grown at 8 °C showed a selective sensitivity regarding utilization of compounds in Prudhoe Bay oil in the presence of the dispersant Corexit 9527. The response was dependent on the nitrogen and phosphate levels of the medium and on the concentration of dispersant used. In the presence of a nitrogen–phosphate solution and a Corexit 9527 – crude oil substrate, degradation of the n-alkanes of the saturate fraction was temporarily retarded in proportion to the concentration of Corexit 9527 present. This retardation was overcome with extended incubation time. In the absence of nitrogen–phosphate supplementation, the effect of Corexit 9527 was pronounced, retarding n-alkane degradation even with extended incubation time. Corexit 9527 had less effect on the degradation of the aromatic fraction and may indeed be stimulatory in the case of select compounds. The development and testing of dispersants containing nitrogen and phosphate is recommended.

Mineralization of [14C]hexadecane and [14C]phenanthrene in crude oil: specificity among bacterial isolates

1990 ◽  
Vol 36 (3) ◽  
pp. 169-175 ◽  
Author(s):  
J. M. Foght ◽  
P. M. Fedorak ◽  
D. W. S. Westlake
Keyword(s):  
Crude Oil ◽  
Aliphatic Compound ◽  
Bacterial Isolates ◽  
Model Compounds ◽  
Degrading Bacteria ◽  
Apparent Correlation ◽  
Oil Components ◽  
The Individual ◽  
Prudhoe Bay ◽  
Degradative Ability

Bacteria isolated from freshwater, marine, and estuarine samples were tested for the ability to produce 14CO2 from n-[1-14C]hexadecane or [9-14C]phenanthrene added to Prudhoe Bay crude oil. Of 138 isolates tested, 54 (39%) mineralized the model aliphatic compound hexadecane and 6 (4%) mineralized the model aromatic compound phenanthrene. None mineralized both compounds. There was no apparent correlation between degradative ability and genus or source. Additional hydrocarbon-degrading bacteria from diverse sources were tested and found to mineralize either hexadecane or phenanthrene. Of 61 hexadecane- and 21 phenanthrene-mineralizing bacteria tested, none mineralized both model compounds. Selected isolates and commercially available cultures were tested for mineralization of specific 14C-labelled mono-, di-, and tri-cyclic aromatics. An apparent hierarchy of degradation was observed: strains mineralizing the mono- and di-cyclic aromatics toluene and naphthalene did not mineralize biphenyl or the tricyclic aromatics anthracene and phenanthrene, whereas those strains that mineralized the tricyclic aromatics also mineralized the smaller substrates. Similarly, not all n-alkane-mineralizing isolates tested mineralized the isoprenoid pristane. A combined culture consisting of one aliphatic- and one aromatic-degrading isolate was tested for mineralization of the model compounds and for degradation of other crude oil components by gas chromatography. No synergism or antagonism was observed compared with degradation by the individual isolates. Key words: aromatic, aliphatic, bioegradation, petroleum.

scholarly journals Biofilm-Mediated Enhanced Crude Oil Degradation by Newly Isolated Pseudomonas Species

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Debdeep Dasgupta ◽  
Ritabrata Ghosh ◽  
Tapas K. Sengupta
Keyword(s):  
Carbon Source ◽  
Crude Oil ◽  
Sole Carbon Source ◽  
River Estuary ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Oil Degradation ◽  
Topographic Analysis ◽  
Degrading Bacteria ◽  
Crude Oil Degradation

The bioavailability of organic contaminants to the degrading bacteria is a major limitation to efficient bioremediation of sites contaminated with hydrophobic pollutants. Such limitation of bioavailability can be overcome by steady-state biofilm-based reactor. The aim of this study was to examine the effect of such multicellular aggregation by naturally existing oil-degrading bacteria on crude oil degradation. Microorganisms, capable of utilizing crude oil as sole carbon source, were isolated from river, estuary and sea-water samples. Biochemical and 16S rDNA analysis of the best degraders of the three sources was found to belong to the Pseudomonas species. Interestingly, one of the isolates was found to be close to Pseudomonas otitidis family which is not reported yet as a degrader of crude oil. Biodegradation of crude oil was estimated by gas chromatography, and biofilm formation near oil-water interface was quantified by confocal laser scanning microscopy. Biofilm supported batches of the isolated Pseudomonas species were able to degrade crude oil much readily and extensively than the planktonic counterparts. Volumetric and topographic analysis revealed that biofilms formed in presence of crude oil accumulate higher biomass with greater thickness compared to the biofilms produced in presence of glucose as sole carbon source.

Characterization of crude oil degrading microbial cultures isolated in Qingdao China

RSC Advances ◽  
2015 ◽  
Vol 5 (118) ◽  
pp. 97665-97674 ◽  
Author(s):  
Yongrui Pi ◽  
Mutai Bao ◽  
Yiming Li ◽  
Guangmei Li ◽  
Jinren Lu ◽  
...  
Keyword(s):  
Carbon Source ◽  
Crude Oil ◽  
Sole Carbon Source ◽  
Sediment Samples ◽  
Microbial Cultures ◽  
Water And Sediment ◽  
Water And Sediment Samples

9 hydrocarbon-degrading strains were isolated based on their ability to grow with crude oil as the sole carbon source from the water and sediment samples of Qingdao offshore.

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