Two vegetable dyes of indigo naturalis and turmeric were combined on dyeing tussah silk fabric with two steps. The first step is dyeing the silk fabric with indigo naturalis by reduction process, and then dyed with turmeric by direct dyeing process. The results of single factor experiment on the indigo naturalis dyed tussah silk fabric dyeing with turmeric indicated that the relative concentration, pH value, dyeing temperature, and dyeing time had effect to a certain extent on the over dyeing. The dyeing conditions were determined by the above single factor experiment. The rubbing fastness and washing resistance of the dyed silk fabric could achieve to grade 3 more than grade 3.