Gene expression profiles in human HepG2 cells treated with extracts of the Tamarindus indica fruit pulp

Traditional Chinese medicine (TCM) has been used for thousands of years. Most Chinese herbal formulae consist of several herbal components and have been used to treat various diseases. However, the mechanisms of most formulae and the relationship between formulae and their components remain to be elucidated. Here we analyzed the putative mechanism of San-Huang-Xie-Xin-Tang (SHXXT) and defined the relationship between SHXXT and its herbal components by microarray technique. HepG2 cells were treated with SHXXT or its components and the gene expression profiles were analyzed by DNA microarray. Gene set enrichment analysis indicated that SHXXT and its components displayed a unique anti-proliferation pattern via p53 signaling, p53 activated, and DNA damage signaling pathways in HepG2 cells. Network analysis showed that most genes were regulated by one molecule, p53. In addition, hierarchical clustering analysis showed that Rhizoma Coptis shared a similar gene expression profile with SHXXT. These findings may explain why Rhizoma Coptis is the principle herb that exerts the major effect in the herbal formula, SHXXT. Moreover, this is the first report to reveal the relationship between formulae and their herbal components in TCM by microarray and bioinformatics tools.

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Comparison of basal gene expression profiles and effects of hepatocarcinogens on gene expression in cultured primary human hepatocytes and HepG2 cells

Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis ◽

10.1016/j.mrfmmm.2003.11.014 ◽

2004 ◽

Vol 549 (1-2) ◽

pp. 79-99 ◽

Cited By ~ 81

Author(s):

Angela J Harris ◽

Stacey L Dial ◽

Daniel A Casciano

Keyword(s):

Gene Expression ◽

Hepg2 Cells ◽

Expression Profiles ◽

Gene Expression Profiles ◽

Human Hepatocytes ◽

Primary Human Hepatocytes

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Different statins produce highly divergent changes in gene expression profiles of human hepatoma cells: a pilot study.

Acta Biochimica Polonica ◽

10.18388/abp.2011_2235 ◽

2011 ◽

Vol 58 (4) ◽

Cited By ~ 9

Author(s):

Agata Leszczynska ◽

Monika Gora ◽

Danuta Plochocka ◽

Grazyna Hoser ◽

Anna Szkopinska ◽

...

Keyword(s):

Gene Expression ◽

Statin Therapy ◽

Hepg2 Cells ◽

Expression Profiles ◽

Gene Expression Profiles ◽

High Dose ◽

Human Hepatoma ◽

Human Hepatoma Cells ◽

Key Enzyme ◽

Coa Reductase

Statins are inhibitors of 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR), the key enzyme of the sterol biosynthesis pathway. Statin therapy is commonly regarded as well tolerated. However, serious adverse effects have also been reported, especially during high-dose statin therapy. The aim of our study was to investigate the effect of statins on gene expression profiles in human hepatoma HepG2 cells using Affymetrix Human Genome U133 Plus 2.0 arrays. Expression of 102, 857 and 1091 genes was changed substantially in HepG2 cells treated with simvastatin, fluvastatin and atorvastatin, respectively. Pathway and gene ontology analysis showed that many of the genes with changed expression levels were involved in a broad range of metabolic processes. The presented data clearly indicate substantial differences between the tested statins.

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Comparison of gene expression profiles of HepG2 cells exposed to Crambescins C1 and A1

Frontiers in Marine Science ◽

10.3389/conf.fmars.2014.02.00061 ◽

2014 ◽

Vol 1 ◽

Author(s):

Sánchez María ◽

Rubiolo Juan ◽

Ternon Eva ◽

Thomas Olivier ◽

Vega Félix ◽

...

Keyword(s):

Gene Expression ◽

Hepg2 Cells ◽

Expression Profiles ◽

Gene Expression Profiles

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Influence of Iron on Cytotoxicity and Gene Expression Profiles Induced by Arsenic in HepG2 Cells

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph16224484 ◽

2019 ◽

Vol 16 (22) ◽

pp. 4484

Author(s):

Yonghua Wang ◽

Yuxuan Liu ◽

Su Liu ◽

Bing Wu

Keyword(s):

Oxidative Stress ◽

Gene Expression ◽

Hepg2 Cells ◽

Expression Profiles ◽

Gene Expression Profiles ◽

Interactive Effects ◽

Lipid Peroxidation Product ◽

Biological Processes ◽

Mitochondrial Depolarization ◽

Peroxidation Product

The toxicity of arsenic (As) could be influenced by many environmental factors and elements. Iron (Fe) is one of the elements that could be involved in As-induced toxicity. In this study, the interactive effects of Fe and As in HepG2 cells were analyzed based on cytotoxicity and transcriptomic analyses. The results showed that Fe could decrease cell viability and increase mitochondrial depolarization induced by As exposure. Oxidative stress and damage have been proven to be one of the main mechanisms of As toxicity. Our results showed that Fe increased the generation of reactive oxygen species (ROS) and lipid peroxidation product malondialdehyde (MDA) induced by As exposure. Microarray analysis further verified that Fe increased the alteration of gene expression and biological processes related to oxidative stress, cell proliferation, and the apoptotic signaling pathway caused by As exposure. Both results of cytotoxicity and transcriptomic analyses suggest that an increase of Fe in the human body could increase the As-induced toxicity, which should be considered during the health risk assessment of As.

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