scholarly journals Kaskadennutzung von Lignocellulose: LX-Verfahren trifft auf B. coagulans

BIOspektrum ◽  
2020 ◽  
Vol 26 (7) ◽  
pp. 803-805
Author(s):  
Linda Schroedter ◽  
Friedrich Streffer ◽  
Katrin Streffer ◽  
Peter Unger ◽  
Joachim Venus
Keyword(s):  
Lactic Acid ◽  
Energy Saving ◽  
Optical Purity ◽  
Bacillus Coagulans ◽  
The Novel ◽  
Corn Straw

AbstractInvestigating alternatives for petrobased substrates, lignocellulose is an interesting yet complex feedstock that offers various possibilities for the design of new and sustainable chemical routes. The novel energy-saving LX-pretreatment was combined with thermophilic Bacillus coagulans. By this, corn straw was used in an innovative cascade obtaining biogas, lignin as well as polymerisable L-(+)-lactic acid of over 99 percents optical purity.

scholarly journals Production and Purification of l-lactic Acid in Lab and Pilot Scales Using Sweet Sorghum Juice

Fermentation ◽  
2019 ◽  
Vol 5 (2) ◽  
pp. 36 ◽  
Author(s):  
Agata Olszewska-Widdrat ◽  
Maria Alexandri ◽  
José Pablo López-Gómez ◽  
Roland Schneider ◽  
Michael Mandl ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Sweet Sorghum ◽  
Lactic Acid Production ◽  
Optical Purity ◽  
Bacillus Coagulans ◽  
Pilot Scale ◽  
Environmentally Benign ◽  
Downstream Process ◽  
Sweet Sorghum Juice ◽  
Ultra Filtration

Sweet sorghum juice (SSJ) was evaluated as fermentation substrate for the production of l-lactic acid. A thermophilic Bacillus coagulans isolate was selected for batch fermentations without the use of additional nutrients. The first batch of SSJ (Batch A) resulted on higher lactic acid concentration, yield and productivity with values of 78.75 g∙L−1, 0.78 g∙g−1 and 1.77 g∙L−1 h−1, respectively. Similar results were obtained when the process was transferred into the pilot scale (50 L), with corresponding values of 73 g∙L−1, 0.70 g∙g−1 and 1.47 g∙L−1 h−1. A complete downstream process scheme was developed in order to separate lactic acid from the fermentation components. Coarse and ultra-filtration were employed as preliminary separation steps. Mono- and bipolar electrodialysis, followed by chromatography and vacuum evaporation were subsequently carried out leading to a solution containing 905.8 g∙L−1 lactic acid, with an optical purity of 98.9%. The results of this study highlight the importance of the downstream process with respect to using SSJ for lactic acid production. The proposed downstream process constitutes a more environmentally benign approach to conventional precipitation methods.

scholarly journals Major Role of NAD-Dependent Lactate Dehydrogenases in the Production of l-Lactic Acid with High Optical Purity by the Thermophile Bacillus coagulans

2014 ◽  
Vol 80 (23) ◽  
pp. 7134-7141 ◽  
Author(s):  
Limin Wang ◽  
Yumeng Cai ◽  
Lingfeng Zhu ◽  
Honglian Guo ◽  
Bo Yu
Keyword(s):  
Lactic Acid ◽  
Lactate Dehydrogenase ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Optical Purity ◽  
Bacillus Coagulans ◽  
Lactobacillus Delbrueckii ◽  
Content Type ◽  
High Optical Purity ◽  
Optically Pure

ABSTRACTBacillus coagulans2-6 is an excellent producer of optically purel-lactic acid. However, little is known about the mechanism of synthesis of the highly optically purel-lactic acid produced by this strain. Three enzymes responsible for lactic acid production—NAD-dependentl-lactate dehydrogenase (l-nLDH; encoded byldhL), NAD-dependentd-lactate dehydrogenase (d-nLDH; encoded byldhD), and glycolate oxidase (GOX)—were systematically investigated in order to study the relationship between these enzymes and the optical purity of lactic acid.Lactobacillus delbrueckiisubsp.bulgaricusDSM 20081 (ad-lactic acid producer) andLactobacillus plantarumsubsp.plantarumDSM 20174 (adl-lactic acid producer) were also examined in this study as comparative strains, in addition toB. coagulans. The specific activities of key enzymes for lactic acid production in the three strains were characterizedin vivoandin vitro, and the levels of transcription of theldhL,ldhD, and GOX genes during fermentation were also analyzed. The catalytic activities ofl-nLDH andd-nLDH were different inl-,d-, anddl-lactic acid producers. Onlyl-nLDH activity was detected inB. coagulans2-6 under native conditions, and the level of transcription ofldhLinB. coagulans2-6 was much higher than that ofldhDor the GOX gene at all growth phases. However, for the twoLactobacillusstrains used in this study,ldhDtranscription levels were higher than those ofldhL. The high catalytic efficiency ofl-nLDH toward pyruvate and the high transcription ratios ofldhLtoldhDandldhLto the GOX gene provide the key explanations for the high optical purity ofl-lactic acid produced byB. coagulans2-6.

scholarly journals L-(+)-Lactic Acid from Reed: Comparing Various Resources for the Nutrient Provision of B. coagulans

Resources ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 89 ◽  
Author(s):  
Linda Schroedter ◽  
Roland Schneider ◽  
Lisa Remus ◽  
Joachim Venus
Keyword(s):  
Lactic Acid ◽  
First Generation ◽  
Low Cost ◽  
Optical Purity ◽  
Value Added ◽  
Bacillus Coagulans ◽  
Common Reed ◽  
Biotechnological Production ◽  
Food And Feed ◽  
Pentose Sugars

Biotechnological production of lactic acid (LA) is based on the so-called first generation feedstocks, meaning sugars derived from food and feed crops such as corn, sugarcane and cassava. The aim of this study was to exploit the potential of a second generation resource: Common reed (Phragmites australis) is a powerfully reproducing sweet grass which grows in wetlands and creates vast monocultural populations. This lignocellulose biomass bears the possibility to be refined to value-added products, without competing with agro industrial land. Besides utilizing reed as a renewable and inexpensive substrate, low-cost nutritional supplementation was analyzed for the fermentation of thermophilic Bacillus coagulans. Various nutritional sources such as baker’s and brewer’s yeast, lucerne green juice and tryptone were investigated for the replacement of yeast extract. The structure of the lignocellulosic material was tackled by chemical treatment (1% NaOH) and enzymatic hydrolysis (Cellic® CTec2). B. coagulans DSM ID 14-300 was employed for the homofermentative conversion of the released hexose and pentose sugars to polymerizable L-(+)-LA of over 99.5% optical purity. The addition of autolyzed baker’s yeast led to the best results of fermentation, enabling an LA titer of 28.3 g L−1 and a yield of 91.6%.

scholarly journals Biorefinery Concept Employing Bacillus coagulans: LX-Lignin and L-(+)-Lactic Acid from Lignocellulose

2021 ◽  
Vol 9 (9) ◽  
pp. 1810
Author(s):  
Linda Schroedter ◽  
Friedrich Streffer ◽  
Katrin Streffer ◽  
Peter Unger ◽  
Joachim Venus
Keyword(s):  
Lactic Acid ◽  
Aromatic Compounds ◽  
Simultaneous Saccharification And Fermentation ◽  
Bacillus Coagulans ◽  
Ash Content ◽  
The Novel ◽  
Fermentative Production ◽  
Low Carbohydrate ◽  
Rye Straw ◽  
Simultaneous Saccharification

A new biorefinery concept is proposed that integrates the novel LX-Pretreatment with the fermentative production of L-(+)-lactic acid. Lignocellulose was chosen as a substrate that does not compete with the provision of food or feed. Furthermore, it contains lignin, a promising new chemical building material which is the largest renewable source for aromatic compounds. Two substrates were investigated: rye straw (RS) as a residue from agriculture, as well as the fibrous digestate of an anaerobic biogas plant operated with energy corn (DCS). Besides the prior production of biogas from energy corn, chemically exploitable LX-Lignin was produced from both sources, creating a product with a low carbohydrate and ash content (90.3% and 88.2% of acid insoluble lignin). Regarding the cellulose fraction of the biomass, enzymatic hydrolysis and fermentation experiments were conducted, comparing a separate (SHF), simultaneous (SSF) and prehydrolyzed simultaneous saccharification and fermentation (PSSF) approach. For this purpose, thermophilic B. coagulans 14-300 was utilized, reaching 38.0 g L−1 LA in 32 h SSF from pretreated RS and 18.3 g L−1 LA in 30 h PSSF from pretreated DCS with optical purities of 99%.

scholarly journals Plant-Based Alternatives to Yogurt: State-of-the-Art and Perspectives of New Biotechnological Challenges

Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 316
Author(s):  
Marco Montemurro ◽  
Erica Pontonio ◽  
Rossana Coda ◽  
Carlo Giuseppe Rizzello
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
State Of The Art ◽  
Protein Digestibility ◽  
The Novel ◽  
Sensory Features ◽  
Long Time ◽  
Biotechnological Processes ◽  
Nutritional Compounds ◽  
Increasing Demand

Due to the increasing demand for milk alternatives, related to both health and ethical needs, plant-based yogurt-like products have been widely explored in recent years. With the main goal to obtain snacks similar to the conventional yogurt in terms of textural and sensory properties and ability to host viable lactic acid bacteria for a long-time storage, several plant-derived ingredients (e.g., cereals, pseudocereals, legumes, and fruits) as well as technological solutions (e.g., enzymatic and thermal treatments) have been investigated. The central role of fermentation in yogurt-like production led to specific selections of lactic acid bacteria strains to be used as starters to guarantee optimal textural (e.g., through the synthesis of exo-polysaccharydes), nutritional (high protein digestibility and low content of anti-nutritional compounds), and functional (synthesis of bioactive compounds) features of the products. This review provides an overview of the novel insights on fermented yogurt-like products. The state-of-the-art on the use of unconventional ingredients, traditional and innovative biotechnological processes, and the effects of fermentation on the textural, nutritional, functional, and sensory features, and the shelf life are described. The supplementation of prebiotics and probiotics and the related health effects are also reviewed.

scholarly journals Draft Genome Sequence of Bacillus coagulans NL01, a Wonderful l-Lactic Acid Producer

2015 ◽  
Vol 3 (3) ◽  
Author(s):  
Zhaojuan Zheng ◽  
Ting Jiang ◽  
Xi Lin ◽  
Jie Zhou ◽  
Jia Ouyang
Keyword(s):  
Lactic Acid ◽  
Genome Sequence ◽  
Draft Genome ◽  
Bacillus Coagulans ◽  
Draft Genome Sequence

scholarly journals A Process Study of Lactic Acid Production from Phragmites australis Straw by a Thermophilic Bacillus coagulans Strain under Non-Sterilized Conditions

Processes ◽  
2018 ◽  
Vol 6 (10) ◽  
pp. 175 ◽  
Author(s):  
Yuming Zhang ◽  
Mengran Li ◽  
Tian Nie ◽  
Zhihua Ni
Keyword(s):  
Lactic Acid ◽  
Phragmites Australis ◽  
Lactic Acid Production ◽  
Scale Up ◽  
Bacillus Coagulans ◽  
Dilute Acid Pretreatment ◽  
Integrated Process ◽  
Dilute Acid ◽  
Acid Pretreatment ◽  
Process Study

Phragmites australis straw (PAS) is an abundant and renewable wetland lignocellulose. Bacillus coagulans IPE22 is a robust thermophilic strain with pentose-utilizing capability and excellent resistance to growth inhibitors. This work is focused on the process study of lactic acid (LA) production from P. australis lignocellulose which has not been attempted previously. By virtue of thermophilic feature of strain IPE22, two fermentation processes (i.e., separated process and integrated process), were developed and compared under non-sterilized conditions. The integrated process combined dilute-acid pretreatment, hemicellulosic hydrolysates fermentation, and cellulose utilization. Sugars derived from hemicellulosic hydrolysates and cellulose enzymatic hydrolysis were efficiently fermented to LA in a single vessel. Using the integrated process, 41.06 g LA was produced from 100 g dry PAS. The established integrated process results in great savings in terms of time and labor, and the fermentation process under non-sterilized conditions is easy to scale up for economical production of lactic acid from PAS.

Energy Saving Preparation and Evaluation of a Novel Polycarboxylate Superplasticizer

2021 ◽  
Vol 1035 ◽  
pp. 1006-1012
Author(s):  
Xiao Liu ◽  
Xiao Fei Song ◽  
Qi Feng Luo ◽  
Chun Lei Xia ◽  
Yun Sheng Zheng ◽  
...  
Keyword(s):  
Energy Saving ◽  
Building Materials ◽  
Equation Model ◽  
Order Kinetic ◽  
The Novel ◽  
Cement Pastes ◽  
Polycarboxylate Superplasticizer ◽  
Low Emission ◽  
Potential Applications ◽  
Preparation And Evaluation

A novel polycarboxylate superplasticizer (PCE) with energy saving preparation was elaborately designed and synthesized by using acrylic acid (AA), hydroxypropyl acrylate (HPA) and isopentenyl polyethylene glycol (IPEG) as monomers. To investigate the effects of the preparation method on the effectiveness of PCE, the PCEs were prepared from energy-saving method and common method respectively, and the hydration heat evolutions of the cement pastes containing these PCEs were comparatively probed. Furthermore, the working mechanisms of the PCEs by different preparations were identified via adsorption behavior, adsorption kinetic and Zeta potential of the PCE on cement surfaces. The results showed that, this novel PCE prepared in an energy saving manner can significantly prolong the hydration process and present a stronger adsorption capacity. In addition, the adsorption of this PCE on cement surface exhibited a characteristic of pseudo first order kinetic equation model. The evaluation in energy conservation showed that, this energy saving preparation can save 1.548×104 kJ per 10 ton production. The aim of this study is to provide a new avenue to synthesize a PCE with economical method which achieves energy-saving preparation. Due to the indispensable application in construction industry, the innovations from this study contribute to the low energy-consumption production and high eco-effectiveness of the novel PCE, which has potential applications in low-emission building materials.

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