scholarly journals Biorefinery Concept Employing Bacillus coagulans: LX-Lignin and L-(+)-Lactic Acid from Lignocellulose

2021 ◽  
Vol 9 (9) ◽  
pp. 1810
Author(s):  
Linda Schroedter ◽  
Friedrich Streffer ◽  
Katrin Streffer ◽  
Peter Unger ◽  
Joachim Venus
Keyword(s):  
Lactic Acid ◽  
Aromatic Compounds ◽  
Simultaneous Saccharification And Fermentation ◽  
Bacillus Coagulans ◽  
Ash Content ◽  
The Novel ◽  
Fermentative Production ◽  
Low Carbohydrate ◽  
Rye Straw ◽  
Simultaneous Saccharification

A new biorefinery concept is proposed that integrates the novel LX-Pretreatment with the fermentative production of L-(+)-lactic acid. Lignocellulose was chosen as a substrate that does not compete with the provision of food or feed. Furthermore, it contains lignin, a promising new chemical building material which is the largest renewable source for aromatic compounds. Two substrates were investigated: rye straw (RS) as a residue from agriculture, as well as the fibrous digestate of an anaerobic biogas plant operated with energy corn (DCS). Besides the prior production of biogas from energy corn, chemically exploitable LX-Lignin was produced from both sources, creating a product with a low carbohydrate and ash content (90.3% and 88.2% of acid insoluble lignin). Regarding the cellulose fraction of the biomass, enzymatic hydrolysis and fermentation experiments were conducted, comparing a separate (SHF), simultaneous (SSF) and prehydrolyzed simultaneous saccharification and fermentation (PSSF) approach. For this purpose, thermophilic B. coagulans 14-300 was utilized, reaching 38.0 g L−1 LA in 32 h SSF from pretreated RS and 18.3 g L−1 LA in 30 h PSSF from pretreated DCS with optical purities of 99%.

scholarly journals Draft Genome Sequence of Bacillus coagulans MA-13, a Thermophilic Lactic Acid Producer from Lignocellulose

2019 ◽  
Vol 8 (23) ◽  
Author(s):  
Martina Aulitto ◽  
Salvatore Fusco ◽  
Carl Johan Franzén ◽  
Andrea Strazzulli ◽  
Marco Moracci ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Genome Sequence ◽  
Simultaneous Saccharification And Fermentation ◽  
Draft Genome ◽  
Bacillus Coagulans ◽  
Draft Genome Sequence ◽  
Content Type ◽  
Lignocellulosic Feedstock ◽  
High Concentrations ◽  
Simultaneous Saccharification

Bacillus coagulans MA-13 is an efficient lactic acid producer which withstands high concentrations of the growth inhibitors formed during the pretreatment of lignocellulosic feedstock. This draft genome sequence is expected to pave the way toward the understanding of mechanisms responsible for the robustness of MA-13 during simultaneous saccharification and fermentation.

scholarly journals Biodetoxification of Phenolic Inhibitors from Lignocellulose Pretreatment using Kurthia huakuii LAM0618T and Subsequent Lactic Acid Fermentation

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2626 ◽  
Author(s):  
Yuejiao Xie ◽  
Qing Hu ◽  
Guodong Feng ◽  
Xu Jiang ◽  
Jinlong Hu ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Corn Stover ◽  
Simultaneous Saccharification And Fermentation ◽  
High Titer ◽  
Bacillus Coagulans ◽  
High Yield ◽  
Acid Fermentation ◽  
Biochemical Production ◽  
Water Rinsing ◽  
Simultaneous Saccharification

Phenolic inhibitors generated during alkaline pretreatment of lignocellulosic biomasses significantly hinder bacterial growth and subsequent biofuel and biochemical production. Water rinsing is an efficient method for removing these compounds. Nevertheless, this method often generates a great amount of wastewater, and leads to the loss of solid fiber particles and fermentable sugars. Kurthia huakuii LAM0618T, a recently identified microorganism, was herein shown to be able to efficiently transform phenolic compounds (syringaldehyde, hydroxybenzaldehyde, and vanillin) into less toxic acids. Taking advantage of these properties, a biodetoxification method was established by inoculating K. huakuii LAM0618T into the NH3/H2O2-pretreated unwashed corn stover to degrade phenolic inhibitors and weak acids generated during the pretreatment. Subsequently, 33.47 and 17.91 g/L lactic acid was produced by Bacillus coagulans LA204 at 50 °C through simultaneous saccharification and fermentation (SSF) from 8% (w/w) of NH3/H2O2-pretreated corn stover with or without K. huakuii LAM0618T-biodetoxification, indicating biodetoxification significantly increased lactic acid titer and yield. Importantly, using 15% (w/w) of the NH3/H2O2-pretreated K. huakuii LAM0618T-biodetoxified corn stover as a substrate through fed-batch simultaneous saccharification and fermentation, high titer and high yield of lactic acid (84.49 g/L and 0.56 g/g corn stover, respectively, with a productivity of 0.88 g/L/h) were produced by Bacillus coagulans LA204. Therefore, this study reported the first study on biodetoxification of alkaline-pretreated lignocellulosic material, and this biodetoxification method could replace water rinsing for removal of phenolic inhibitors and applied in biofuel and biochemical production using the alkaline-pretreated lignocellulosic bioresources.

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